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In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells

In current in vitro study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence...

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Autores principales: Zlatska, Alona, Gordiienko, Inna, Vasyliev, Roman, Zubov, Dmitriy, Gubar, Olga, Rodnichenko, Anzhela, Syroeshkin, Anton, Zlatskiy, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241234/
https://www.ncbi.nlm.nih.gov/pubmed/30519147
http://dx.doi.org/10.1155/2018/5454367
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author Zlatska, Alona
Gordiienko, Inna
Vasyliev, Roman
Zubov, Dmitriy
Gubar, Olga
Rodnichenko, Anzhela
Syroeshkin, Anton
Zlatskiy, Igor
author_facet Zlatska, Alona
Gordiienko, Inna
Vasyliev, Roman
Zubov, Dmitriy
Gubar, Olga
Rodnichenko, Anzhela
Syroeshkin, Anton
Zlatskiy, Igor
author_sort Zlatska, Alona
collection PubMed
description In current in vitro study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence but acquired polygonal morphology with pronounced stress fibers. At high deuterium concentrations the ADSCs population doubling time increased which indicated the cell cycle retardation and decrease of cell proliferation rate. The deuterated and deuterium-depleted growth media demonstrated acute and chronic cytotoxicity, respectively. The minimal migration ability was observed in deuterated medium whereas the highest migration activity was observed in the medium with the deuterium content close to natural. The cells in deuterated growth medium demonstrated decrease in metabolic activity after three days in culture. In contrast, in deuterium-depleted medium there was an increase in ADSC metabolic activity.
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spelling pubmed-62412342018-12-05 In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells Zlatska, Alona Gordiienko, Inna Vasyliev, Roman Zubov, Dmitriy Gubar, Olga Rodnichenko, Anzhela Syroeshkin, Anton Zlatskiy, Igor ScientificWorldJournal Research Article In current in vitro study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence but acquired polygonal morphology with pronounced stress fibers. At high deuterium concentrations the ADSCs population doubling time increased which indicated the cell cycle retardation and decrease of cell proliferation rate. The deuterated and deuterium-depleted growth media demonstrated acute and chronic cytotoxicity, respectively. The minimal migration ability was observed in deuterated medium whereas the highest migration activity was observed in the medium with the deuterium content close to natural. The cells in deuterated growth medium demonstrated decrease in metabolic activity after three days in culture. In contrast, in deuterium-depleted medium there was an increase in ADSC metabolic activity. Hindawi 2018-11-04 /pmc/articles/PMC6241234/ /pubmed/30519147 http://dx.doi.org/10.1155/2018/5454367 Text en Copyright © 2018 Alona Zlatska et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zlatska, Alona
Gordiienko, Inna
Vasyliev, Roman
Zubov, Dmitriy
Gubar, Olga
Rodnichenko, Anzhela
Syroeshkin, Anton
Zlatskiy, Igor
In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells
title In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells
title_full In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells
title_fullStr In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells
title_full_unstemmed In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells
title_short In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells
title_sort in vitro study of deuterium effect on biological properties of human cultured adipose-derived stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241234/
https://www.ncbi.nlm.nih.gov/pubmed/30519147
http://dx.doi.org/10.1155/2018/5454367
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