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beadplexr: reproducible and automated analysis of multiplex bead assays
Multiplex bead assays are an extension of the commonly used sandwich ELISA. The advantage over ELISA is that they make simultaneous evaluation of several analytes possible. Several commercial assay systems, where the beads are acquired on a standard flow cytometer, exist. These assay systems come wi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241392/ https://www.ncbi.nlm.nih.gov/pubmed/30479885 http://dx.doi.org/10.7717/peerj.5794 |
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author | Stervbo, Ulrik Westhoff, Timm H. Babel, Nina |
author_facet | Stervbo, Ulrik Westhoff, Timm H. Babel, Nina |
author_sort | Stervbo, Ulrik |
collection | PubMed |
description | Multiplex bead assays are an extension of the commonly used sandwich ELISA. The advantage over ELISA is that they make simultaneous evaluation of several analytes possible. Several commercial assay systems, where the beads are acquired on a standard flow cytometer, exist. These assay systems come with their own software tool for analysis and evaluation of the concentration of the analyzed analytes. However, these tools are either tied to particular commercial software or impose other limitations to their licenses, such as the number of events which can be analyzed. In addition, all these solutions are ‘point and click’ which potentially obscures the steps taken in the analysis. Here we present beadplexer, an open-source R-package for the reproducible analysis of multiplex bead assay data. The package makes it possible to automatically identify bead clusters, and provides functionality to easily fit a standard curve and calculate the concentrations of the analyzed analytes. beadplexer is available from CRAN and from https://gitlab.com/ustervbo/beadplexr. |
format | Online Article Text |
id | pubmed-6241392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62413922018-11-26 beadplexr: reproducible and automated analysis of multiplex bead assays Stervbo, Ulrik Westhoff, Timm H. Babel, Nina PeerJ Immunology Multiplex bead assays are an extension of the commonly used sandwich ELISA. The advantage over ELISA is that they make simultaneous evaluation of several analytes possible. Several commercial assay systems, where the beads are acquired on a standard flow cytometer, exist. These assay systems come with their own software tool for analysis and evaluation of the concentration of the analyzed analytes. However, these tools are either tied to particular commercial software or impose other limitations to their licenses, such as the number of events which can be analyzed. In addition, all these solutions are ‘point and click’ which potentially obscures the steps taken in the analysis. Here we present beadplexer, an open-source R-package for the reproducible analysis of multiplex bead assay data. The package makes it possible to automatically identify bead clusters, and provides functionality to easily fit a standard curve and calculate the concentrations of the analyzed analytes. beadplexer is available from CRAN and from https://gitlab.com/ustervbo/beadplexr. PeerJ Inc. 2018-11-16 /pmc/articles/PMC6241392/ /pubmed/30479885 http://dx.doi.org/10.7717/peerj.5794 Text en ©2018 Stervbo et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Immunology Stervbo, Ulrik Westhoff, Timm H. Babel, Nina beadplexr: reproducible and automated analysis of multiplex bead assays |
title | beadplexr: reproducible and automated analysis of multiplex bead assays |
title_full | beadplexr: reproducible and automated analysis of multiplex bead assays |
title_fullStr | beadplexr: reproducible and automated analysis of multiplex bead assays |
title_full_unstemmed | beadplexr: reproducible and automated analysis of multiplex bead assays |
title_short | beadplexr: reproducible and automated analysis of multiplex bead assays |
title_sort | beadplexr: reproducible and automated analysis of multiplex bead assays |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6241392/ https://www.ncbi.nlm.nih.gov/pubmed/30479885 http://dx.doi.org/10.7717/peerj.5794 |
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