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A Catalytic and Selective Scissoring Molecular Tool for Quadruplex Nucleic Acids
[Image: see text] A copper complex embedded in the structure of a water-soluble naphthalene diimide has been designed to bind and cleave G-quadruplex DNA. We describe the properties of this ligand, including its catalytic activity in the generation of ROS. FRET melting, CD, NMR, gel sequencing, and...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical
Society
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242190/ https://www.ncbi.nlm.nih.gov/pubmed/30351011 http://dx.doi.org/10.1021/jacs.8b05337 |
| _version_ | 1783371810641805312 |
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| author | Nadai, Matteo Doria, Filippo Scalabrin, Matteo Pirota, Valentina Grande, Vincenzo Bergamaschi, Greta Amendola, Valeria Winnerdy, Fernaldo Richtia Phan, Anh Tuân Richter, Sara N. Freccero, Mauro |
| author_facet | Nadai, Matteo Doria, Filippo Scalabrin, Matteo Pirota, Valentina Grande, Vincenzo Bergamaschi, Greta Amendola, Valeria Winnerdy, Fernaldo Richtia Phan, Anh Tuân Richter, Sara N. Freccero, Mauro |
| author_sort | Nadai, Matteo |
| collection | PubMed |
| description | [Image: see text] A copper complex embedded in the structure of a water-soluble naphthalene diimide has been designed to bind and cleave G-quadruplex DNA. We describe the properties of this ligand, including its catalytic activity in the generation of ROS. FRET melting, CD, NMR, gel sequencing, and mass spectrometry experiments highlight a unique and unexpected selectivity in cleaving G-quadruplex sequences. This selectivity relies both on the binding affinity and structural features of the targeted G-quadruplexes. |
| format | Online Article Text |
| id | pubmed-6242190 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2018 |
| publisher | American Chemical
Society |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-62421902018-11-20 A Catalytic and Selective Scissoring Molecular Tool for Quadruplex Nucleic Acids Nadai, Matteo Doria, Filippo Scalabrin, Matteo Pirota, Valentina Grande, Vincenzo Bergamaschi, Greta Amendola, Valeria Winnerdy, Fernaldo Richtia Phan, Anh Tuân Richter, Sara N. Freccero, Mauro J Am Chem Soc [Image: see text] A copper complex embedded in the structure of a water-soluble naphthalene diimide has been designed to bind and cleave G-quadruplex DNA. We describe the properties of this ligand, including its catalytic activity in the generation of ROS. FRET melting, CD, NMR, gel sequencing, and mass spectrometry experiments highlight a unique and unexpected selectivity in cleaving G-quadruplex sequences. This selectivity relies both on the binding affinity and structural features of the targeted G-quadruplexes. American Chemical Society 2018-10-14 2018-11-07 /pmc/articles/PMC6242190/ /pubmed/30351011 http://dx.doi.org/10.1021/jacs.8b05337 Text en Copyright © 2018 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
| spellingShingle | Nadai, Matteo Doria, Filippo Scalabrin, Matteo Pirota, Valentina Grande, Vincenzo Bergamaschi, Greta Amendola, Valeria Winnerdy, Fernaldo Richtia Phan, Anh Tuân Richter, Sara N. Freccero, Mauro A Catalytic and Selective Scissoring Molecular Tool for Quadruplex Nucleic Acids |
| title | A Catalytic
and Selective Scissoring Molecular Tool
for Quadruplex Nucleic Acids |
| title_full | A Catalytic
and Selective Scissoring Molecular Tool
for Quadruplex Nucleic Acids |
| title_fullStr | A Catalytic
and Selective Scissoring Molecular Tool
for Quadruplex Nucleic Acids |
| title_full_unstemmed | A Catalytic
and Selective Scissoring Molecular Tool
for Quadruplex Nucleic Acids |
| title_short | A Catalytic
and Selective Scissoring Molecular Tool
for Quadruplex Nucleic Acids |
| title_sort | catalytic
and selective scissoring molecular tool
for quadruplex nucleic acids |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242190/ https://www.ncbi.nlm.nih.gov/pubmed/30351011 http://dx.doi.org/10.1021/jacs.8b05337 |
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