Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors

High-throughput genetic screens are powerful methods to identify genes linked to a given phenotype. The catalytic null mutant of the Cas9 RNA-guided nuclease (dCas9) can be conveniently used to silence genes of interest in a method also known as CRISPRi. Here, we report a genome-wide CRISPR-dCas9 sc...

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Detalles Bibliográficos
Autores principales: Rousset, François, Cui, Lun, Siouve, Elise, Becavin, Christophe, Depardieu, Florence, Bikard, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242692/
https://www.ncbi.nlm.nih.gov/pubmed/30403660
http://dx.doi.org/10.1371/journal.pgen.1007749

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242692/
https://www.ncbi.nlm.nih.gov/pubmed/30403660
http://dx.doi.org/10.1371/journal.pgen.1007749

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