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Increased Abundance of Lactobacillales in the Colon of Beta-Adrenergic Receptor Knock Out Mouse Is Associated With Increased Gut Bacterial Production of Short Chain Fatty Acids and Reduced IL17 Expression in Circulating CD4(+) Immune Cells

Emerging evidence suggests an associative link between gut dysbiosis, the autonomic nervous system (ANS) and the immune system in pathophysiology of neurogenic hypertension (HTN). However, the close interplay between these three systems presents us with difficulties in deciphering the cause-effect r...

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Autores principales: Bartley, Akeem, Yang, Tao, Arocha, Rebeca, Malphurs, Wendi L., Larkin, Riley, Magee, Kacy L., Vickroy, Thomas W., Zubcevic, Jasenka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242911/
https://www.ncbi.nlm.nih.gov/pubmed/30483153
http://dx.doi.org/10.3389/fphys.2018.01593
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author Bartley, Akeem
Yang, Tao
Arocha, Rebeca
Malphurs, Wendi L.
Larkin, Riley
Magee, Kacy L.
Vickroy, Thomas W.
Zubcevic, Jasenka
author_facet Bartley, Akeem
Yang, Tao
Arocha, Rebeca
Malphurs, Wendi L.
Larkin, Riley
Magee, Kacy L.
Vickroy, Thomas W.
Zubcevic, Jasenka
author_sort Bartley, Akeem
collection PubMed
description Emerging evidence suggests an associative link between gut dysbiosis, the autonomic nervous system (ANS) and the immune system in pathophysiology of neurogenic hypertension (HTN). However, the close interplay between these three systems presents us with difficulties in deciphering the cause-effect relationship in disease. The present study utilized beta 1 and 2 adrenergic receptor knock out (AdrB1(tm1Bkk)AdrB2(tm1Bkk)/J KO) mice to isolate the effects of reduced overall sympathetic drive on gut microbiota and systemic immune system. We observed the following: (i) Diminished beta adrenergic signaling mainly reflects in shifts in the Firmicutes phyla, with a significant increase in abundance of largely beneficial Bacilli Lactobacillales in the KO mice; (ii) This was associated with increased colonic production of beneficial short chain fatty acids (SCFAs) butyrate, acetate and propionate, confirming functional microbiota shifts in the KO mice; (iii) Dampened systemic immune responses in the KO mice reflected in reduction on circulating CD4(+).IL17(+) T cells and increase in young neutrophils, both previously associated with shifts in the gut microbiota. Taken together, these observations demonstrate that reduced expression of beta adrenergic receptors may lead to beneficial shifts in the gut microbiota and dampened systemic immune responses. Considering the role of both in hypertension, this suggests that dietary intervention may be a viable option for manipulation of blood pressure via correcting gut dysbiosis.
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spelling pubmed-62429112018-11-27 Increased Abundance of Lactobacillales in the Colon of Beta-Adrenergic Receptor Knock Out Mouse Is Associated With Increased Gut Bacterial Production of Short Chain Fatty Acids and Reduced IL17 Expression in Circulating CD4(+) Immune Cells Bartley, Akeem Yang, Tao Arocha, Rebeca Malphurs, Wendi L. Larkin, Riley Magee, Kacy L. Vickroy, Thomas W. Zubcevic, Jasenka Front Physiol Physiology Emerging evidence suggests an associative link between gut dysbiosis, the autonomic nervous system (ANS) and the immune system in pathophysiology of neurogenic hypertension (HTN). However, the close interplay between these three systems presents us with difficulties in deciphering the cause-effect relationship in disease. The present study utilized beta 1 and 2 adrenergic receptor knock out (AdrB1(tm1Bkk)AdrB2(tm1Bkk)/J KO) mice to isolate the effects of reduced overall sympathetic drive on gut microbiota and systemic immune system. We observed the following: (i) Diminished beta adrenergic signaling mainly reflects in shifts in the Firmicutes phyla, with a significant increase in abundance of largely beneficial Bacilli Lactobacillales in the KO mice; (ii) This was associated with increased colonic production of beneficial short chain fatty acids (SCFAs) butyrate, acetate and propionate, confirming functional microbiota shifts in the KO mice; (iii) Dampened systemic immune responses in the KO mice reflected in reduction on circulating CD4(+).IL17(+) T cells and increase in young neutrophils, both previously associated with shifts in the gut microbiota. Taken together, these observations demonstrate that reduced expression of beta adrenergic receptors may lead to beneficial shifts in the gut microbiota and dampened systemic immune responses. Considering the role of both in hypertension, this suggests that dietary intervention may be a viable option for manipulation of blood pressure via correcting gut dysbiosis. Frontiers Media S.A. 2018-11-13 /pmc/articles/PMC6242911/ /pubmed/30483153 http://dx.doi.org/10.3389/fphys.2018.01593 Text en Copyright © 2018 Bartley, Yang, Arocha, Malphurs, Larkin, Magee, Vickroy and Zubcevic. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Bartley, Akeem
Yang, Tao
Arocha, Rebeca
Malphurs, Wendi L.
Larkin, Riley
Magee, Kacy L.
Vickroy, Thomas W.
Zubcevic, Jasenka
Increased Abundance of Lactobacillales in the Colon of Beta-Adrenergic Receptor Knock Out Mouse Is Associated With Increased Gut Bacterial Production of Short Chain Fatty Acids and Reduced IL17 Expression in Circulating CD4(+) Immune Cells
title Increased Abundance of Lactobacillales in the Colon of Beta-Adrenergic Receptor Knock Out Mouse Is Associated With Increased Gut Bacterial Production of Short Chain Fatty Acids and Reduced IL17 Expression in Circulating CD4(+) Immune Cells
title_full Increased Abundance of Lactobacillales in the Colon of Beta-Adrenergic Receptor Knock Out Mouse Is Associated With Increased Gut Bacterial Production of Short Chain Fatty Acids and Reduced IL17 Expression in Circulating CD4(+) Immune Cells
title_fullStr Increased Abundance of Lactobacillales in the Colon of Beta-Adrenergic Receptor Knock Out Mouse Is Associated With Increased Gut Bacterial Production of Short Chain Fatty Acids and Reduced IL17 Expression in Circulating CD4(+) Immune Cells
title_full_unstemmed Increased Abundance of Lactobacillales in the Colon of Beta-Adrenergic Receptor Knock Out Mouse Is Associated With Increased Gut Bacterial Production of Short Chain Fatty Acids and Reduced IL17 Expression in Circulating CD4(+) Immune Cells
title_short Increased Abundance of Lactobacillales in the Colon of Beta-Adrenergic Receptor Knock Out Mouse Is Associated With Increased Gut Bacterial Production of Short Chain Fatty Acids and Reduced IL17 Expression in Circulating CD4(+) Immune Cells
title_sort increased abundance of lactobacillales in the colon of beta-adrenergic receptor knock out mouse is associated with increased gut bacterial production of short chain fatty acids and reduced il17 expression in circulating cd4(+) immune cells
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242911/
https://www.ncbi.nlm.nih.gov/pubmed/30483153
http://dx.doi.org/10.3389/fphys.2018.01593
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