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Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells
Limited access to human islets has prompted the development of human beta cell models. The human beta cell lines EndoC-βH1 and EndoC-βH2 are increasingly used by the research community. However, little is known of their electrophysiological and secretory properties. Here, we monitored parameters tha...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242937/ https://www.ncbi.nlm.nih.gov/pubmed/30451893 http://dx.doi.org/10.1038/s41598-018-34743-7 |
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author | Hastoy, Benoît Godazgar, Mahdieh Clark, Anne Nylander, Vibe Spiliotis, Ioannis van de Bunt, Martijn Chibalina, Margarita V. Barrett, Amy Burrows, Carla Tarasov, Andrei I. Scharfmann, Raphael Gloyn, Anna L. Rorsman, Patrik |
author_facet | Hastoy, Benoît Godazgar, Mahdieh Clark, Anne Nylander, Vibe Spiliotis, Ioannis van de Bunt, Martijn Chibalina, Margarita V. Barrett, Amy Burrows, Carla Tarasov, Andrei I. Scharfmann, Raphael Gloyn, Anna L. Rorsman, Patrik |
author_sort | Hastoy, Benoît |
collection | PubMed |
description | Limited access to human islets has prompted the development of human beta cell models. The human beta cell lines EndoC-βH1 and EndoC-βH2 are increasingly used by the research community. However, little is known of their electrophysiological and secretory properties. Here, we monitored parameters that constitute the glucose-triggering pathway of insulin release. Both cell lines respond to glucose (6 and 20 mM) with 2- to 3-fold stimulation of insulin secretion which correlated with an elevation of [Ca(2+)](i), membrane depolarisation and increased action potential firing. Similar to human primary beta cells, K(ATP) channel activity is low at 1 mM glucose and is further reduced upon increasing glucose concentration; an effect that was mimicked by the K(ATP) channel blocker tolbutamide. The upstroke of the action potentials reflects the activation of Ca(2+) channels with some small contribution of TTX-sensitive Na(+) channels. The repolarisation involves activation of voltage-gated Kv2.2 channels and large-conductance Ca(2+)-activated K(+) channels. Exocytosis presented a similar kinetics to human primary beta cells. The ultrastructure of these cells shows insulin vesicles composed of an electron-dense core surrounded by a thin clear halo. We conclude that the EndoC-βH1 and -βH2 cells share many features of primary human β-cells and thus represent a useful experimental model. |
format | Online Article Text |
id | pubmed-6242937 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62429372018-11-27 Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells Hastoy, Benoît Godazgar, Mahdieh Clark, Anne Nylander, Vibe Spiliotis, Ioannis van de Bunt, Martijn Chibalina, Margarita V. Barrett, Amy Burrows, Carla Tarasov, Andrei I. Scharfmann, Raphael Gloyn, Anna L. Rorsman, Patrik Sci Rep Article Limited access to human islets has prompted the development of human beta cell models. The human beta cell lines EndoC-βH1 and EndoC-βH2 are increasingly used by the research community. However, little is known of their electrophysiological and secretory properties. Here, we monitored parameters that constitute the glucose-triggering pathway of insulin release. Both cell lines respond to glucose (6 and 20 mM) with 2- to 3-fold stimulation of insulin secretion which correlated with an elevation of [Ca(2+)](i), membrane depolarisation and increased action potential firing. Similar to human primary beta cells, K(ATP) channel activity is low at 1 mM glucose and is further reduced upon increasing glucose concentration; an effect that was mimicked by the K(ATP) channel blocker tolbutamide. The upstroke of the action potentials reflects the activation of Ca(2+) channels with some small contribution of TTX-sensitive Na(+) channels. The repolarisation involves activation of voltage-gated Kv2.2 channels and large-conductance Ca(2+)-activated K(+) channels. Exocytosis presented a similar kinetics to human primary beta cells. The ultrastructure of these cells shows insulin vesicles composed of an electron-dense core surrounded by a thin clear halo. We conclude that the EndoC-βH1 and -βH2 cells share many features of primary human β-cells and thus represent a useful experimental model. Nature Publishing Group UK 2018-11-19 /pmc/articles/PMC6242937/ /pubmed/30451893 http://dx.doi.org/10.1038/s41598-018-34743-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Hastoy, Benoît Godazgar, Mahdieh Clark, Anne Nylander, Vibe Spiliotis, Ioannis van de Bunt, Martijn Chibalina, Margarita V. Barrett, Amy Burrows, Carla Tarasov, Andrei I. Scharfmann, Raphael Gloyn, Anna L. Rorsman, Patrik Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells |
title | Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells |
title_full | Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells |
title_fullStr | Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells |
title_full_unstemmed | Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells |
title_short | Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells |
title_sort | electrophysiological properties of human beta-cell lines endoc-βh1 and -βh2 conform with human beta-cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242937/ https://www.ncbi.nlm.nih.gov/pubmed/30451893 http://dx.doi.org/10.1038/s41598-018-34743-7 |
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