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Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives

Arid regions show relatively fewer species in comparison to better-watered biomes, but the competition for the few nutrients is very distinct. Here, in total 373 bacterial strains were isolated from rhizospheric soils obtained from three different sampling sites in Tunisia. Their potential for the p...

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Autores principales: Nasfi, Zina, Busch, Henrik, Kehraus, Stefan, Linares-Otoya, Luis, König, Gabriele M., Schäberle, Till F., Bachoual, Rafik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242944/
https://www.ncbi.nlm.nih.gov/pubmed/30483240
http://dx.doi.org/10.3389/fmicb.2018.02742
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author Nasfi, Zina
Busch, Henrik
Kehraus, Stefan
Linares-Otoya, Luis
König, Gabriele M.
Schäberle, Till F.
Bachoual, Rafik
author_facet Nasfi, Zina
Busch, Henrik
Kehraus, Stefan
Linares-Otoya, Luis
König, Gabriele M.
Schäberle, Till F.
Bachoual, Rafik
author_sort Nasfi, Zina
collection PubMed
description Arid regions show relatively fewer species in comparison to better-watered biomes, but the competition for the few nutrients is very distinct. Here, in total 373 bacterial strains were isolated from rhizospheric soils obtained from three different sampling sites in Tunisia. Their potential for the production of antimicrobial compounds was evaluated. Bacterial strains, showing antibacterial activity against pathogenic bacteria, were isolated from all three sites, one strain from the Bou-Hedma national park, 15 strains from Chott-Djerid, and 13 strains from Matmata, respectively. The dominant genus was Bacillus, with 27 out of 29 strains. Most interestingly, 93% of the isolates showed activity against Gram-positive and Gram-negative test bacteria. Strain Bacillus sp. M21, harboring high inhibitory potential, even against clinical isolates of Gram-negative bacteria, was analyzed in detail to enable purification and identification of the bioactive compound responsible for its bioactivity. Subsequent HPLC-MS and NMR analyses resulted in the identification of 1-acetyl-β-carboline as active component. Furthermore, fungicides of the bacillomycin and fengycin group, which in addition show antibiotic effects, were identified. This work highlights the high potential of the arid-adapted strains for the biosynthesis of specialized metabolites and suggest further investigation of extreme environments, since they constitute a promising bioresource of biologically active compounds.
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spelling pubmed-62429442018-11-27 Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives Nasfi, Zina Busch, Henrik Kehraus, Stefan Linares-Otoya, Luis König, Gabriele M. Schäberle, Till F. Bachoual, Rafik Front Microbiol Microbiology Arid regions show relatively fewer species in comparison to better-watered biomes, but the competition for the few nutrients is very distinct. Here, in total 373 bacterial strains were isolated from rhizospheric soils obtained from three different sampling sites in Tunisia. Their potential for the production of antimicrobial compounds was evaluated. Bacterial strains, showing antibacterial activity against pathogenic bacteria, were isolated from all three sites, one strain from the Bou-Hedma national park, 15 strains from Chott-Djerid, and 13 strains from Matmata, respectively. The dominant genus was Bacillus, with 27 out of 29 strains. Most interestingly, 93% of the isolates showed activity against Gram-positive and Gram-negative test bacteria. Strain Bacillus sp. M21, harboring high inhibitory potential, even against clinical isolates of Gram-negative bacteria, was analyzed in detail to enable purification and identification of the bioactive compound responsible for its bioactivity. Subsequent HPLC-MS and NMR analyses resulted in the identification of 1-acetyl-β-carboline as active component. Furthermore, fungicides of the bacillomycin and fengycin group, which in addition show antibiotic effects, were identified. This work highlights the high potential of the arid-adapted strains for the biosynthesis of specialized metabolites and suggest further investigation of extreme environments, since they constitute a promising bioresource of biologically active compounds. Frontiers Media S.A. 2018-11-13 /pmc/articles/PMC6242944/ /pubmed/30483240 http://dx.doi.org/10.3389/fmicb.2018.02742 Text en Copyright © 2018 Nasfi, Busch, Kehraus, Linares-Otoya, König, Schäberle and Bachoual. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Nasfi, Zina
Busch, Henrik
Kehraus, Stefan
Linares-Otoya, Luis
König, Gabriele M.
Schäberle, Till F.
Bachoual, Rafik
Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives
title Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives
title_full Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives
title_fullStr Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives
title_full_unstemmed Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives
title_short Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives
title_sort soil bacteria isolated from tunisian arid areas show promising antimicrobial activities against gram-negatives
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242944/
https://www.ncbi.nlm.nih.gov/pubmed/30483240
http://dx.doi.org/10.3389/fmicb.2018.02742
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