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A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids
Ultraviolet light is the dominant environmental oxidative skin stressor and a major skin aging factor. We studied which oxidized phospholipid (OxPL) mediators would be generated in primary human keratinocytes (KC) upon exposure to ultraviolet A light (UVA) and investigated the contribution of OxPL t...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6243031/ https://www.ncbi.nlm.nih.gov/pubmed/30466060 http://dx.doi.org/10.1016/j.redox.2018.11.006 |
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author | Narzt, Marie-Sophie Nagelreiter, Ionela-Mariana Oskolkova, Olga Bochkov, Valery N. Latreille, Julie Fedorova, Maria Ni, Zhixu Sialana, Fernando J. Lubec, Gert Filzwieser, Manuel Laggner, Maria Bilban, Martin Mildner, Michael Tschachler, Erwin Grillari, Johannes Gruber, Florian |
author_facet | Narzt, Marie-Sophie Nagelreiter, Ionela-Mariana Oskolkova, Olga Bochkov, Valery N. Latreille, Julie Fedorova, Maria Ni, Zhixu Sialana, Fernando J. Lubec, Gert Filzwieser, Manuel Laggner, Maria Bilban, Martin Mildner, Michael Tschachler, Erwin Grillari, Johannes Gruber, Florian |
author_sort | Narzt, Marie-Sophie |
collection | PubMed |
description | Ultraviolet light is the dominant environmental oxidative skin stressor and a major skin aging factor. We studied which oxidized phospholipid (OxPL) mediators would be generated in primary human keratinocytes (KC) upon exposure to ultraviolet A light (UVA) and investigated the contribution of OxPL to UVA responses. Mass spectrometric analysis immediately or 24 h post UV stress revealed significant changes in abundance of 173 and 84 lipid species, respectively. We identified known and novel lipid species including known bioactive and also potentially reactive carbonyl containing species. We found indication for selective metabolism and degradation of selected reactive lipids. Exposure to both UVA and to in vitro UVA - oxidized phospholipids activated, on transcriptome and proteome level, NRF2/antioxidant response signaling, lipid metabolizing enzyme expression and unfolded protein response (UPR) signaling. We identified NUPR1 as an upstream regulator of UVA/OxPL transcriptional stress responses and found this protein to be expressed in the epidermis. Silencing of NUPR1 resulted in augmented expression of antioxidant and lipid detoxification genes and disturbed the cell cycle, making it a potential key factor in skin reactive oxygen species (ROS) responses intimately involved in aging and pathology. |
format | Online Article Text |
id | pubmed-6243031 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-62430312018-11-26 A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids Narzt, Marie-Sophie Nagelreiter, Ionela-Mariana Oskolkova, Olga Bochkov, Valery N. Latreille, Julie Fedorova, Maria Ni, Zhixu Sialana, Fernando J. Lubec, Gert Filzwieser, Manuel Laggner, Maria Bilban, Martin Mildner, Michael Tschachler, Erwin Grillari, Johannes Gruber, Florian Redox Biol Research Paper Ultraviolet light is the dominant environmental oxidative skin stressor and a major skin aging factor. We studied which oxidized phospholipid (OxPL) mediators would be generated in primary human keratinocytes (KC) upon exposure to ultraviolet A light (UVA) and investigated the contribution of OxPL to UVA responses. Mass spectrometric analysis immediately or 24 h post UV stress revealed significant changes in abundance of 173 and 84 lipid species, respectively. We identified known and novel lipid species including known bioactive and also potentially reactive carbonyl containing species. We found indication for selective metabolism and degradation of selected reactive lipids. Exposure to both UVA and to in vitro UVA - oxidized phospholipids activated, on transcriptome and proteome level, NRF2/antioxidant response signaling, lipid metabolizing enzyme expression and unfolded protein response (UPR) signaling. We identified NUPR1 as an upstream regulator of UVA/OxPL transcriptional stress responses and found this protein to be expressed in the epidermis. Silencing of NUPR1 resulted in augmented expression of antioxidant and lipid detoxification genes and disturbed the cell cycle, making it a potential key factor in skin reactive oxygen species (ROS) responses intimately involved in aging and pathology. Elsevier 2018-11-13 /pmc/articles/PMC6243031/ /pubmed/30466060 http://dx.doi.org/10.1016/j.redox.2018.11.006 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Paper Narzt, Marie-Sophie Nagelreiter, Ionela-Mariana Oskolkova, Olga Bochkov, Valery N. Latreille, Julie Fedorova, Maria Ni, Zhixu Sialana, Fernando J. Lubec, Gert Filzwieser, Manuel Laggner, Maria Bilban, Martin Mildner, Michael Tschachler, Erwin Grillari, Johannes Gruber, Florian A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids |
title | A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids |
title_full | A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids |
title_fullStr | A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids |
title_full_unstemmed | A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids |
title_short | A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids |
title_sort | novel role for nupr1 in the keratinocyte stress response to uv oxidized phospholipids |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6243031/ https://www.ncbi.nlm.nih.gov/pubmed/30466060 http://dx.doi.org/10.1016/j.redox.2018.11.006 |
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