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A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids

Ultraviolet light is the dominant environmental oxidative skin stressor and a major skin aging factor. We studied which oxidized phospholipid (OxPL) mediators would be generated in primary human keratinocytes (KC) upon exposure to ultraviolet A light (UVA) and investigated the contribution of OxPL t...

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Autores principales: Narzt, Marie-Sophie, Nagelreiter, Ionela-Mariana, Oskolkova, Olga, Bochkov, Valery N., Latreille, Julie, Fedorova, Maria, Ni, Zhixu, Sialana, Fernando J., Lubec, Gert, Filzwieser, Manuel, Laggner, Maria, Bilban, Martin, Mildner, Michael, Tschachler, Erwin, Grillari, Johannes, Gruber, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6243031/
https://www.ncbi.nlm.nih.gov/pubmed/30466060
http://dx.doi.org/10.1016/j.redox.2018.11.006
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author Narzt, Marie-Sophie
Nagelreiter, Ionela-Mariana
Oskolkova, Olga
Bochkov, Valery N.
Latreille, Julie
Fedorova, Maria
Ni, Zhixu
Sialana, Fernando J.
Lubec, Gert
Filzwieser, Manuel
Laggner, Maria
Bilban, Martin
Mildner, Michael
Tschachler, Erwin
Grillari, Johannes
Gruber, Florian
author_facet Narzt, Marie-Sophie
Nagelreiter, Ionela-Mariana
Oskolkova, Olga
Bochkov, Valery N.
Latreille, Julie
Fedorova, Maria
Ni, Zhixu
Sialana, Fernando J.
Lubec, Gert
Filzwieser, Manuel
Laggner, Maria
Bilban, Martin
Mildner, Michael
Tschachler, Erwin
Grillari, Johannes
Gruber, Florian
author_sort Narzt, Marie-Sophie
collection PubMed
description Ultraviolet light is the dominant environmental oxidative skin stressor and a major skin aging factor. We studied which oxidized phospholipid (OxPL) mediators would be generated in primary human keratinocytes (KC) upon exposure to ultraviolet A light (UVA) and investigated the contribution of OxPL to UVA responses. Mass spectrometric analysis immediately or 24 h post UV stress revealed significant changes in abundance of 173 and 84 lipid species, respectively. We identified known and novel lipid species including known bioactive and also potentially reactive carbonyl containing species. We found indication for selective metabolism and degradation of selected reactive lipids. Exposure to both UVA and to in vitro UVA - oxidized phospholipids activated, on transcriptome and proteome level, NRF2/antioxidant response signaling, lipid metabolizing enzyme expression and unfolded protein response (UPR) signaling. We identified NUPR1 as an upstream regulator of UVA/OxPL transcriptional stress responses and found this protein to be expressed in the epidermis. Silencing of NUPR1 resulted in augmented expression of antioxidant and lipid detoxification genes and disturbed the cell cycle, making it a potential key factor in skin reactive oxygen species (ROS) responses intimately involved in aging and pathology.
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spelling pubmed-62430312018-11-26 A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids Narzt, Marie-Sophie Nagelreiter, Ionela-Mariana Oskolkova, Olga Bochkov, Valery N. Latreille, Julie Fedorova, Maria Ni, Zhixu Sialana, Fernando J. Lubec, Gert Filzwieser, Manuel Laggner, Maria Bilban, Martin Mildner, Michael Tschachler, Erwin Grillari, Johannes Gruber, Florian Redox Biol Research Paper Ultraviolet light is the dominant environmental oxidative skin stressor and a major skin aging factor. We studied which oxidized phospholipid (OxPL) mediators would be generated in primary human keratinocytes (KC) upon exposure to ultraviolet A light (UVA) and investigated the contribution of OxPL to UVA responses. Mass spectrometric analysis immediately or 24 h post UV stress revealed significant changes in abundance of 173 and 84 lipid species, respectively. We identified known and novel lipid species including known bioactive and also potentially reactive carbonyl containing species. We found indication for selective metabolism and degradation of selected reactive lipids. Exposure to both UVA and to in vitro UVA - oxidized phospholipids activated, on transcriptome and proteome level, NRF2/antioxidant response signaling, lipid metabolizing enzyme expression and unfolded protein response (UPR) signaling. We identified NUPR1 as an upstream regulator of UVA/OxPL transcriptional stress responses and found this protein to be expressed in the epidermis. Silencing of NUPR1 resulted in augmented expression of antioxidant and lipid detoxification genes and disturbed the cell cycle, making it a potential key factor in skin reactive oxygen species (ROS) responses intimately involved in aging and pathology. Elsevier 2018-11-13 /pmc/articles/PMC6243031/ /pubmed/30466060 http://dx.doi.org/10.1016/j.redox.2018.11.006 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Paper
Narzt, Marie-Sophie
Nagelreiter, Ionela-Mariana
Oskolkova, Olga
Bochkov, Valery N.
Latreille, Julie
Fedorova, Maria
Ni, Zhixu
Sialana, Fernando J.
Lubec, Gert
Filzwieser, Manuel
Laggner, Maria
Bilban, Martin
Mildner, Michael
Tschachler, Erwin
Grillari, Johannes
Gruber, Florian
A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids
title A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids
title_full A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids
title_fullStr A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids
title_full_unstemmed A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids
title_short A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids
title_sort novel role for nupr1 in the keratinocyte stress response to uv oxidized phospholipids
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6243031/
https://www.ncbi.nlm.nih.gov/pubmed/30466060
http://dx.doi.org/10.1016/j.redox.2018.11.006
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