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The First Survey of Isolation and Molecular Typing of Toxoplasma gondii by Bioassay and PCR Method in BALB/c Mice in Camels (Camelus dromedarius) from Eastern Iran

BACKGROUND: We isolated Toxoplasma gondii from camels by bioassay method in mice model and detect parasitic DNA in brain mice by molecular methods. METHODS: One hundred tissue samples including heart (n=50), and diaphragm (n=50) were collected from camels (n=50) slaughtered in abattoirs from Feb to...

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Autores principales: TAVAKOLI KARESHK, Amir, TAVAKOLI OLIAEE, Razieh, MAHMOUDVAND, Hossein, KEYHANI, Amir, MOHAMMADI, Mohammad Ali, BAMOROVAT, Mehdi, HAJHOSSEINI, Mohammad Ali, ZIA-ALI, Naser
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6243161/
https://www.ncbi.nlm.nih.gov/pubmed/30483329
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author TAVAKOLI KARESHK, Amir
TAVAKOLI OLIAEE, Razieh
MAHMOUDVAND, Hossein
KEYHANI, Amir
MOHAMMADI, Mohammad Ali
BAMOROVAT, Mehdi
HAJHOSSEINI, Mohammad Ali
ZIA-ALI, Naser
author_facet TAVAKOLI KARESHK, Amir
TAVAKOLI OLIAEE, Razieh
MAHMOUDVAND, Hossein
KEYHANI, Amir
MOHAMMADI, Mohammad Ali
BAMOROVAT, Mehdi
HAJHOSSEINI, Mohammad Ali
ZIA-ALI, Naser
author_sort TAVAKOLI KARESHK, Amir
collection PubMed
description BACKGROUND: We isolated Toxoplasma gondii from camels by bioassay method in mice model and detect parasitic DNA in brain mice by molecular methods. METHODS: One hundred tissue samples including heart (n=50), and diaphragm (n=50) were collected from camels (n=50) slaughtered in abattoirs from Feb to Oct 2015 in three provinces located in eastern Iran. In first, blood sample from 50 camels was assayed for anti-Toxoplasma antibodies by modified agglutination test (MAT) test. Bioassay method was done in positive MAT blood camels in BALB/c mice and Nested PCR performed in seropositive tissue samples to amplify the B1 and GRA6 genes. The existence of polymorphic restriction sites for endonuclease MseI was used with PCRRFLP method and Sequencing analysis to evaluate the prevalence of type strains (I, II and III) RESULTS: Overall, 13 (26%) of camels were positive with titer of 1:20 for toxoplasmosis and 13(26%) tissue samples of camels were found positive for the T. gondii B1 gene, including 7(14%) diaphragm, 6(12%) heart. Moreover, 3(6%) tissue samples of camels were found positive with GRA6 gene for T. gondii. There are three genotypes and mix genotype using MseI enzyme among all positive samples. CONCLUSION: The obtained results from serological and molecular tests demonstrated the infection of T. gondii with previously recognized genotypes in the tissues of camels for first time from Iran. Since consumption of meat camels are raising in Iran, there may be a high risk of toxoplasmosis through consumption of products from these hosts due to their susceptibility to the infection.
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spelling pubmed-62431612018-11-27 The First Survey of Isolation and Molecular Typing of Toxoplasma gondii by Bioassay and PCR Method in BALB/c Mice in Camels (Camelus dromedarius) from Eastern Iran TAVAKOLI KARESHK, Amir TAVAKOLI OLIAEE, Razieh MAHMOUDVAND, Hossein KEYHANI, Amir MOHAMMADI, Mohammad Ali BAMOROVAT, Mehdi HAJHOSSEINI, Mohammad Ali ZIA-ALI, Naser Iran J Parasitol Original Article BACKGROUND: We isolated Toxoplasma gondii from camels by bioassay method in mice model and detect parasitic DNA in brain mice by molecular methods. METHODS: One hundred tissue samples including heart (n=50), and diaphragm (n=50) were collected from camels (n=50) slaughtered in abattoirs from Feb to Oct 2015 in three provinces located in eastern Iran. In first, blood sample from 50 camels was assayed for anti-Toxoplasma antibodies by modified agglutination test (MAT) test. Bioassay method was done in positive MAT blood camels in BALB/c mice and Nested PCR performed in seropositive tissue samples to amplify the B1 and GRA6 genes. The existence of polymorphic restriction sites for endonuclease MseI was used with PCRRFLP method and Sequencing analysis to evaluate the prevalence of type strains (I, II and III) RESULTS: Overall, 13 (26%) of camels were positive with titer of 1:20 for toxoplasmosis and 13(26%) tissue samples of camels were found positive for the T. gondii B1 gene, including 7(14%) diaphragm, 6(12%) heart. Moreover, 3(6%) tissue samples of camels were found positive with GRA6 gene for T. gondii. There are three genotypes and mix genotype using MseI enzyme among all positive samples. CONCLUSION: The obtained results from serological and molecular tests demonstrated the infection of T. gondii with previously recognized genotypes in the tissues of camels for first time from Iran. Since consumption of meat camels are raising in Iran, there may be a high risk of toxoplasmosis through consumption of products from these hosts due to their susceptibility to the infection. Tehran University of Medical Sciences 2018 /pmc/articles/PMC6243161/ /pubmed/30483329 Text en Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
TAVAKOLI KARESHK, Amir
TAVAKOLI OLIAEE, Razieh
MAHMOUDVAND, Hossein
KEYHANI, Amir
MOHAMMADI, Mohammad Ali
BAMOROVAT, Mehdi
HAJHOSSEINI, Mohammad Ali
ZIA-ALI, Naser
The First Survey of Isolation and Molecular Typing of Toxoplasma gondii by Bioassay and PCR Method in BALB/c Mice in Camels (Camelus dromedarius) from Eastern Iran
title The First Survey of Isolation and Molecular Typing of Toxoplasma gondii by Bioassay and PCR Method in BALB/c Mice in Camels (Camelus dromedarius) from Eastern Iran
title_full The First Survey of Isolation and Molecular Typing of Toxoplasma gondii by Bioassay and PCR Method in BALB/c Mice in Camels (Camelus dromedarius) from Eastern Iran
title_fullStr The First Survey of Isolation and Molecular Typing of Toxoplasma gondii by Bioassay and PCR Method in BALB/c Mice in Camels (Camelus dromedarius) from Eastern Iran
title_full_unstemmed The First Survey of Isolation and Molecular Typing of Toxoplasma gondii by Bioassay and PCR Method in BALB/c Mice in Camels (Camelus dromedarius) from Eastern Iran
title_short The First Survey of Isolation and Molecular Typing of Toxoplasma gondii by Bioassay and PCR Method in BALB/c Mice in Camels (Camelus dromedarius) from Eastern Iran
title_sort first survey of isolation and molecular typing of toxoplasma gondii by bioassay and pcr method in balb/c mice in camels (camelus dromedarius) from eastern iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6243161/
https://www.ncbi.nlm.nih.gov/pubmed/30483329
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