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Molecular Identification and Phylogenetic Classification of Leishmania spp. Isolated from Human Cutaneous Leishmaniasis in Iran: A Cross-sectional Study

BACKGROUND: In Iran, both forms of cutaneous (CL) and visceral leishmaniasis (VL) have been reported; so the accurate species identification of the parasite(s) and the analysis of genetic diversity are necessary. METHODS: The smears were collected from lesions samples of 654 patients with CL, who at...

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Autores principales: MOHAMMADIHA, Anita, DALIMI, Abdolhossein, MOHEBALI, Mehdi, SHARIFI, Iraj, MAHMOUDI, Mohammadreza, MIRZAEI, Asad, SPOTIN, Adel, BEHRAVAN, Mahmoodreza, KARIMI, Mehdi, ARBABI, Mohsen, NEKOEIAN, Shahram, KALANTARI, Reza, GHORBANZADEH, Behzad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6243174/
https://www.ncbi.nlm.nih.gov/pubmed/30483325
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author MOHAMMADIHA, Anita
DALIMI, Abdolhossein
MOHEBALI, Mehdi
SHARIFI, Iraj
MAHMOUDI, Mohammadreza
MIRZAEI, Asad
SPOTIN, Adel
BEHRAVAN, Mahmoodreza
KARIMI, Mehdi
ARBABI, Mohsen
NEKOEIAN, Shahram
KALANTARI, Reza
GHORBANZADEH, Behzad
author_facet MOHAMMADIHA, Anita
DALIMI, Abdolhossein
MOHEBALI, Mehdi
SHARIFI, Iraj
MAHMOUDI, Mohammadreza
MIRZAEI, Asad
SPOTIN, Adel
BEHRAVAN, Mahmoodreza
KARIMI, Mehdi
ARBABI, Mohsen
NEKOEIAN, Shahram
KALANTARI, Reza
GHORBANZADEH, Behzad
author_sort MOHAMMADIHA, Anita
collection PubMed
description BACKGROUND: In Iran, both forms of cutaneous (CL) and visceral leishmaniasis (VL) have been reported; so the accurate species identification of the parasite(s) and the analysis of genetic diversity are necessary. METHODS: The smears were collected from lesions samples of 654 patients with CL, who attended local health centers in 12 provinces of Iran during 2013–2015. The smears were checked for the presence of amastigotes by light microscopy. DNA of 648 Leishmania isolates, amplified by targeting a partial sequence of ITS (18S rRNA–ITS1–5.8S rRNA–ITS2) gene. Twenty-five of all the amplicons were sequenced and analyzed with restriction fragment length polymorphism (RFLP) using the Taq1 enzyme. RESULTS: All the smears were positive microscopically. The PCR-RFLP analysis revealed that 176 (27%) CL patients were infected with L. tropica and, 478 (73%) with L. major. The dominant species in all over Iran is L. major. The sequencing results of all CL patients and RFLP analysis confirmed each other. Based on our phylogenetic tree, 25 ITS DNA sequences were grouped into two clusters representing L. major and L. tropica species. Phylogenetic tree derived from the ITS sequences supports a clear divergence between L. major from the other species. CONCLUSION: Discrimination of Iranian Leishmania isolates using ITS gene gives us this opportunity to detect, identify, and construct the phylogenetic relationship of Iranian isolates.
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spelling pubmed-62431742018-11-27 Molecular Identification and Phylogenetic Classification of Leishmania spp. Isolated from Human Cutaneous Leishmaniasis in Iran: A Cross-sectional Study MOHAMMADIHA, Anita DALIMI, Abdolhossein MOHEBALI, Mehdi SHARIFI, Iraj MAHMOUDI, Mohammadreza MIRZAEI, Asad SPOTIN, Adel BEHRAVAN, Mahmoodreza KARIMI, Mehdi ARBABI, Mohsen NEKOEIAN, Shahram KALANTARI, Reza GHORBANZADEH, Behzad Iran J Parasitol Original Article BACKGROUND: In Iran, both forms of cutaneous (CL) and visceral leishmaniasis (VL) have been reported; so the accurate species identification of the parasite(s) and the analysis of genetic diversity are necessary. METHODS: The smears were collected from lesions samples of 654 patients with CL, who attended local health centers in 12 provinces of Iran during 2013–2015. The smears were checked for the presence of amastigotes by light microscopy. DNA of 648 Leishmania isolates, amplified by targeting a partial sequence of ITS (18S rRNA–ITS1–5.8S rRNA–ITS2) gene. Twenty-five of all the amplicons were sequenced and analyzed with restriction fragment length polymorphism (RFLP) using the Taq1 enzyme. RESULTS: All the smears were positive microscopically. The PCR-RFLP analysis revealed that 176 (27%) CL patients were infected with L. tropica and, 478 (73%) with L. major. The dominant species in all over Iran is L. major. The sequencing results of all CL patients and RFLP analysis confirmed each other. Based on our phylogenetic tree, 25 ITS DNA sequences were grouped into two clusters representing L. major and L. tropica species. Phylogenetic tree derived from the ITS sequences supports a clear divergence between L. major from the other species. CONCLUSION: Discrimination of Iranian Leishmania isolates using ITS gene gives us this opportunity to detect, identify, and construct the phylogenetic relationship of Iranian isolates. Tehran University of Medical Sciences 2018 /pmc/articles/PMC6243174/ /pubmed/30483325 Text en Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
MOHAMMADIHA, Anita
DALIMI, Abdolhossein
MOHEBALI, Mehdi
SHARIFI, Iraj
MAHMOUDI, Mohammadreza
MIRZAEI, Asad
SPOTIN, Adel
BEHRAVAN, Mahmoodreza
KARIMI, Mehdi
ARBABI, Mohsen
NEKOEIAN, Shahram
KALANTARI, Reza
GHORBANZADEH, Behzad
Molecular Identification and Phylogenetic Classification of Leishmania spp. Isolated from Human Cutaneous Leishmaniasis in Iran: A Cross-sectional Study
title Molecular Identification and Phylogenetic Classification of Leishmania spp. Isolated from Human Cutaneous Leishmaniasis in Iran: A Cross-sectional Study
title_full Molecular Identification and Phylogenetic Classification of Leishmania spp. Isolated from Human Cutaneous Leishmaniasis in Iran: A Cross-sectional Study
title_fullStr Molecular Identification and Phylogenetic Classification of Leishmania spp. Isolated from Human Cutaneous Leishmaniasis in Iran: A Cross-sectional Study
title_full_unstemmed Molecular Identification and Phylogenetic Classification of Leishmania spp. Isolated from Human Cutaneous Leishmaniasis in Iran: A Cross-sectional Study
title_short Molecular Identification and Phylogenetic Classification of Leishmania spp. Isolated from Human Cutaneous Leishmaniasis in Iran: A Cross-sectional Study
title_sort molecular identification and phylogenetic classification of leishmania spp. isolated from human cutaneous leishmaniasis in iran: a cross-sectional study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6243174/
https://www.ncbi.nlm.nih.gov/pubmed/30483325
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