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Structural basis for anthrax toxin receptor 1 recognition by Seneca Valley Virus

Recently, the use of oncolytic viruses in cancer therapy has become a realistic therapeutic option. Seneca Valley Virus (SVV) is a newly discovered picornavirus, which has earned a significant reputation as a potent oncolytic agent. Anthrax toxin receptor 1 (ANTXR1), one of the cellular receptors fo...

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Autores principales: Jayawardena, Nadishka, Burga, Laura N., Easingwood, Richard A., Takizawa, Yoshimasa, Wolf, Matthias, Bostina, Mihnea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6243253/
https://www.ncbi.nlm.nih.gov/pubmed/30381454
http://dx.doi.org/10.1073/pnas.1810664115
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author Jayawardena, Nadishka
Burga, Laura N.
Easingwood, Richard A.
Takizawa, Yoshimasa
Wolf, Matthias
Bostina, Mihnea
author_facet Jayawardena, Nadishka
Burga, Laura N.
Easingwood, Richard A.
Takizawa, Yoshimasa
Wolf, Matthias
Bostina, Mihnea
author_sort Jayawardena, Nadishka
collection PubMed
description Recently, the use of oncolytic viruses in cancer therapy has become a realistic therapeutic option. Seneca Valley Virus (SVV) is a newly discovered picornavirus, which has earned a significant reputation as a potent oncolytic agent. Anthrax toxin receptor 1 (ANTXR1), one of the cellular receptors for the protective antigen secreted by Bacillus anthracis, has been identified as the high-affinity cellular receptor for SVV. Here, we report the structure of the SVV-ANTXR1 complex determined by single-particle cryo-electron microscopy analysis at near-atomic resolution. This is an example of a shared receptor structure between a mammalian virus and a bacterial toxin. Our structure shows that ANTXR1 decorates the outer surface of the SVV capsid and interacts with the surface-exposed BC loop and loop II of VP1, “the puff” of VP2 and “the knob” of VP3. Comparison of the receptor-bound capsid structure with the native capsid structure reveals that receptor binding induces minor conformational changes in SVV capsid structure, suggesting the role of ANTXR1 as an attachment receptor. Furthermore, our results demonstrate that the capsid footprint on the receptor is not conserved in anthrax toxin receptor 2 (ANTXR2), thereby providing a molecular mechanism for explaining the exquisite selectivity of SVV for ANTXR1.
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spelling pubmed-62432532018-11-27 Structural basis for anthrax toxin receptor 1 recognition by Seneca Valley Virus Jayawardena, Nadishka Burga, Laura N. Easingwood, Richard A. Takizawa, Yoshimasa Wolf, Matthias Bostina, Mihnea Proc Natl Acad Sci U S A PNAS Plus Recently, the use of oncolytic viruses in cancer therapy has become a realistic therapeutic option. Seneca Valley Virus (SVV) is a newly discovered picornavirus, which has earned a significant reputation as a potent oncolytic agent. Anthrax toxin receptor 1 (ANTXR1), one of the cellular receptors for the protective antigen secreted by Bacillus anthracis, has been identified as the high-affinity cellular receptor for SVV. Here, we report the structure of the SVV-ANTXR1 complex determined by single-particle cryo-electron microscopy analysis at near-atomic resolution. This is an example of a shared receptor structure between a mammalian virus and a bacterial toxin. Our structure shows that ANTXR1 decorates the outer surface of the SVV capsid and interacts with the surface-exposed BC loop and loop II of VP1, “the puff” of VP2 and “the knob” of VP3. Comparison of the receptor-bound capsid structure with the native capsid structure reveals that receptor binding induces minor conformational changes in SVV capsid structure, suggesting the role of ANTXR1 as an attachment receptor. Furthermore, our results demonstrate that the capsid footprint on the receptor is not conserved in anthrax toxin receptor 2 (ANTXR2), thereby providing a molecular mechanism for explaining the exquisite selectivity of SVV for ANTXR1. National Academy of Sciences 2018-11-13 2018-10-31 /pmc/articles/PMC6243253/ /pubmed/30381454 http://dx.doi.org/10.1073/pnas.1810664115 Text en Copyright © 2018 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle PNAS Plus
Jayawardena, Nadishka
Burga, Laura N.
Easingwood, Richard A.
Takizawa, Yoshimasa
Wolf, Matthias
Bostina, Mihnea
Structural basis for anthrax toxin receptor 1 recognition by Seneca Valley Virus
title Structural basis for anthrax toxin receptor 1 recognition by Seneca Valley Virus
title_full Structural basis for anthrax toxin receptor 1 recognition by Seneca Valley Virus
title_fullStr Structural basis for anthrax toxin receptor 1 recognition by Seneca Valley Virus
title_full_unstemmed Structural basis for anthrax toxin receptor 1 recognition by Seneca Valley Virus
title_short Structural basis for anthrax toxin receptor 1 recognition by Seneca Valley Virus
title_sort structural basis for anthrax toxin receptor 1 recognition by seneca valley virus
topic PNAS Plus
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6243253/
https://www.ncbi.nlm.nih.gov/pubmed/30381454
http://dx.doi.org/10.1073/pnas.1810664115
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