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Mapping heterogeneous polarity in multicompartment nanoparticles

Understanding polarity gradients inside nanomaterials is essential to capture their potential as nanoreactors, catalysts or in drug delivery applications. We propose here a method to obtain detailed, quantitative information on heterogeneous polarity in multicompartment nanostructures. The method is...

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Autores principales: Palomba, Francesco, Genovese, Damiano, Petrizza, Luca, Rampazzo, Enrico, Zaccheroni, Nelsi, Prodi, Luca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6244083/
https://www.ncbi.nlm.nih.gov/pubmed/30459427
http://dx.doi.org/10.1038/s41598-018-35257-y
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author Palomba, Francesco
Genovese, Damiano
Petrizza, Luca
Rampazzo, Enrico
Zaccheroni, Nelsi
Prodi, Luca
author_facet Palomba, Francesco
Genovese, Damiano
Petrizza, Luca
Rampazzo, Enrico
Zaccheroni, Nelsi
Prodi, Luca
author_sort Palomba, Francesco
collection PubMed
description Understanding polarity gradients inside nanomaterials is essential to capture their potential as nanoreactors, catalysts or in drug delivery applications. We propose here a method to obtain detailed, quantitative information on heterogeneous polarity in multicompartment nanostructures. The method is based on a 2-steps procedure, (i) deconvolution of complex emission spectra of two solvatochromic probes followed by (ii) spectrally resolved analysis of FRET between the same solvatochromic dyes. While the first step yields a list of polarities probed in the nanomaterial suspension, the second step correlates the polarities in space. Colocalization of polarities falling within few nanometer radius is obtained via FRET, a process called here nanopolarity mapping. Here, Prodan and Nile Red are tested to map the polarity of a water-dispersable, multicompartment nanostructure, named PluS nanoparticle (NPs). PluS NPs are uniform core-shell nanoparticles with silica cores (diameter ~10 nm) and Pluronic F127 shell (thickness ~7 nm). The probes report on a wide range of nanopolarities among which the dyes efficiently exchange energy via FRET, demonstrating the coexistence of a rich variety of environments within nanometer distance. Their use as a FRET couple highlights the proximity of strongly hydrophobic sites and hydrated layers, and quantitatively accounts for the emission component related to external water, which remains unaffected by FRET processes. This method is general and applicable to map nanopolarity in a large variety of nanomaterials.
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spelling pubmed-62440832018-11-27 Mapping heterogeneous polarity in multicompartment nanoparticles Palomba, Francesco Genovese, Damiano Petrizza, Luca Rampazzo, Enrico Zaccheroni, Nelsi Prodi, Luca Sci Rep Article Understanding polarity gradients inside nanomaterials is essential to capture their potential as nanoreactors, catalysts or in drug delivery applications. We propose here a method to obtain detailed, quantitative information on heterogeneous polarity in multicompartment nanostructures. The method is based on a 2-steps procedure, (i) deconvolution of complex emission spectra of two solvatochromic probes followed by (ii) spectrally resolved analysis of FRET between the same solvatochromic dyes. While the first step yields a list of polarities probed in the nanomaterial suspension, the second step correlates the polarities in space. Colocalization of polarities falling within few nanometer radius is obtained via FRET, a process called here nanopolarity mapping. Here, Prodan and Nile Red are tested to map the polarity of a water-dispersable, multicompartment nanostructure, named PluS nanoparticle (NPs). PluS NPs are uniform core-shell nanoparticles with silica cores (diameter ~10 nm) and Pluronic F127 shell (thickness ~7 nm). The probes report on a wide range of nanopolarities among which the dyes efficiently exchange energy via FRET, demonstrating the coexistence of a rich variety of environments within nanometer distance. Their use as a FRET couple highlights the proximity of strongly hydrophobic sites and hydrated layers, and quantitatively accounts for the emission component related to external water, which remains unaffected by FRET processes. This method is general and applicable to map nanopolarity in a large variety of nanomaterials. Nature Publishing Group UK 2018-11-20 /pmc/articles/PMC6244083/ /pubmed/30459427 http://dx.doi.org/10.1038/s41598-018-35257-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Palomba, Francesco
Genovese, Damiano
Petrizza, Luca
Rampazzo, Enrico
Zaccheroni, Nelsi
Prodi, Luca
Mapping heterogeneous polarity in multicompartment nanoparticles
title Mapping heterogeneous polarity in multicompartment nanoparticles
title_full Mapping heterogeneous polarity in multicompartment nanoparticles
title_fullStr Mapping heterogeneous polarity in multicompartment nanoparticles
title_full_unstemmed Mapping heterogeneous polarity in multicompartment nanoparticles
title_short Mapping heterogeneous polarity in multicompartment nanoparticles
title_sort mapping heterogeneous polarity in multicompartment nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6244083/
https://www.ncbi.nlm.nih.gov/pubmed/30459427
http://dx.doi.org/10.1038/s41598-018-35257-y
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