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Enhanced fluorescent intensity of magnetic-fluorescent bifunctional PLGA microspheres based on Janus electrospraying for bioapplication
Microspheres with magnetic-fluorescent functions have received attention due to fluorescent tracking and target positioning. To improve the accuracy of optical imaging and the fluorescent tracking of drug release, it is essential to enhance the fluorescent intensity of microparticles. Magnetic-fluor...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6244220/ https://www.ncbi.nlm.nih.gov/pubmed/30459341 http://dx.doi.org/10.1038/s41598-018-34856-z |
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author | Li, Kun Li, Ping Jia, Zhengtai Qi, Bing Xu, Junwei Kang, Danyue Liu, Meili Fan, Yubo |
author_facet | Li, Kun Li, Ping Jia, Zhengtai Qi, Bing Xu, Junwei Kang, Danyue Liu, Meili Fan, Yubo |
author_sort | Li, Kun |
collection | PubMed |
description | Microspheres with magnetic-fluorescent functions have received attention due to fluorescent tracking and target positioning. To improve the accuracy of optical imaging and the fluorescent tracking of drug release, it is essential to enhance the fluorescent intensity of microparticles. Magnetic-fluorescent bifunctional poly lactic-co-glycolic acid (PLGA) Janus microspheres [PLGA/TbLa(3)(Bim)(12)]//[PLGA/Fe(3)O(4)] with double chambers were fabricated with the double-needle electrospraying method. The fluorescent drug TbLa(3)(Bim)(12) with dual rare earth ions was encapsulated in one chamber, while Fe(3)O(4) magnetic nanoparticles (Fe(3)O(4) MNPs) were simultaneously encapsulated in another chamber. In comparison, magnetic-fluorescent PLGA composite microspheres PLGA/TbLa(3)(Bim)(12)/Fe(3)O(4) were also prepared, which encapsulated fluorescent drugs TbLa(3)(Bim)(12) with dual rare earth (RE) ions and Fe(3)O(4) MNPs in one chamber. The fluorescent intensity at 542 nm of Janus microspheres was about three times higher than that of composite microspheres due to a decrease in contact between fluorescent-labeling RE drug and MNPs. The fluorescent intensities of Janus microspheres with different contents of Fe(3)O(4) MNPs and TbLa(3)(Bim)(12) were investigated. Furthermore, the magnetic properties, thermostability, cell toxicity and hemolytic properties of Janus microspheres were also assayed to conduct a tentative exploration of their bioapplication. The Janus microspheres provide many opportunities for application in biofields such as drug delivery. |
format | Online Article Text |
id | pubmed-6244220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62442202018-11-28 Enhanced fluorescent intensity of magnetic-fluorescent bifunctional PLGA microspheres based on Janus electrospraying for bioapplication Li, Kun Li, Ping Jia, Zhengtai Qi, Bing Xu, Junwei Kang, Danyue Liu, Meili Fan, Yubo Sci Rep Article Microspheres with magnetic-fluorescent functions have received attention due to fluorescent tracking and target positioning. To improve the accuracy of optical imaging and the fluorescent tracking of drug release, it is essential to enhance the fluorescent intensity of microparticles. Magnetic-fluorescent bifunctional poly lactic-co-glycolic acid (PLGA) Janus microspheres [PLGA/TbLa(3)(Bim)(12)]//[PLGA/Fe(3)O(4)] with double chambers were fabricated with the double-needle electrospraying method. The fluorescent drug TbLa(3)(Bim)(12) with dual rare earth ions was encapsulated in one chamber, while Fe(3)O(4) magnetic nanoparticles (Fe(3)O(4) MNPs) were simultaneously encapsulated in another chamber. In comparison, magnetic-fluorescent PLGA composite microspheres PLGA/TbLa(3)(Bim)(12)/Fe(3)O(4) were also prepared, which encapsulated fluorescent drugs TbLa(3)(Bim)(12) with dual rare earth (RE) ions and Fe(3)O(4) MNPs in one chamber. The fluorescent intensity at 542 nm of Janus microspheres was about three times higher than that of composite microspheres due to a decrease in contact between fluorescent-labeling RE drug and MNPs. The fluorescent intensities of Janus microspheres with different contents of Fe(3)O(4) MNPs and TbLa(3)(Bim)(12) were investigated. Furthermore, the magnetic properties, thermostability, cell toxicity and hemolytic properties of Janus microspheres were also assayed to conduct a tentative exploration of their bioapplication. The Janus microspheres provide many opportunities for application in biofields such as drug delivery. Nature Publishing Group UK 2018-11-20 /pmc/articles/PMC6244220/ /pubmed/30459341 http://dx.doi.org/10.1038/s41598-018-34856-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Li, Kun Li, Ping Jia, Zhengtai Qi, Bing Xu, Junwei Kang, Danyue Liu, Meili Fan, Yubo Enhanced fluorescent intensity of magnetic-fluorescent bifunctional PLGA microspheres based on Janus electrospraying for bioapplication |
title | Enhanced fluorescent intensity of magnetic-fluorescent bifunctional PLGA microspheres based on Janus electrospraying for bioapplication |
title_full | Enhanced fluorescent intensity of magnetic-fluorescent bifunctional PLGA microspheres based on Janus electrospraying for bioapplication |
title_fullStr | Enhanced fluorescent intensity of magnetic-fluorescent bifunctional PLGA microspheres based on Janus electrospraying for bioapplication |
title_full_unstemmed | Enhanced fluorescent intensity of magnetic-fluorescent bifunctional PLGA microspheres based on Janus electrospraying for bioapplication |
title_short | Enhanced fluorescent intensity of magnetic-fluorescent bifunctional PLGA microspheres based on Janus electrospraying for bioapplication |
title_sort | enhanced fluorescent intensity of magnetic-fluorescent bifunctional plga microspheres based on janus electrospraying for bioapplication |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6244220/ https://www.ncbi.nlm.nih.gov/pubmed/30459341 http://dx.doi.org/10.1038/s41598-018-34856-z |
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