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Expression of the POTE gene family in human ovarian cancer

The POTE family includes 14 genes in three phylogenetic groups. We determined POTE mRNA expression in normal tissues, epithelial ovarian and high-grade serous ovarian cancer (EOC, HGSC), and pan-cancer, and determined the relationship of POTE expression to ovarian cancer clinicopathology. Groups 1 &...

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Autores principales: Barger, Carter J, Zhang, Wa, Sharma, Ashok, Chee, Linda, James, Smitha R., Kufel, Christina N., Miller, Austin, Meza, Jane, Drapkin, Ronny, Odunsi, Kunle, Klinkebiel, David, Karpf, Adam R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6244393/
https://www.ncbi.nlm.nih.gov/pubmed/30459449
http://dx.doi.org/10.1038/s41598-018-35567-1
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author Barger, Carter J
Zhang, Wa
Sharma, Ashok
Chee, Linda
James, Smitha R.
Kufel, Christina N.
Miller, Austin
Meza, Jane
Drapkin, Ronny
Odunsi, Kunle
Klinkebiel, David
Karpf, Adam R.
author_facet Barger, Carter J
Zhang, Wa
Sharma, Ashok
Chee, Linda
James, Smitha R.
Kufel, Christina N.
Miller, Austin
Meza, Jane
Drapkin, Ronny
Odunsi, Kunle
Klinkebiel, David
Karpf, Adam R.
author_sort Barger, Carter J
collection PubMed
description The POTE family includes 14 genes in three phylogenetic groups. We determined POTE mRNA expression in normal tissues, epithelial ovarian and high-grade serous ovarian cancer (EOC, HGSC), and pan-cancer, and determined the relationship of POTE expression to ovarian cancer clinicopathology. Groups 1 & 2 POTEs showed testis-specific expression in normal tissues, consistent with assignment as cancer-testis antigens (CTAs), while Group 3 POTEs were expressed in several normal tissues, indicating they are not CTAs. Pan-POTE and individual POTEs showed significantly elevated expression in EOC and HGSC compared to normal controls. Pan-POTE correlated with increased stage, grade, and the HGSC subtype. Select individual POTEs showed increased expression in recurrent HGSC, and POTEE specifically associated with reduced HGSC OS. Consistent with tumors, EOC cell lines had significantly elevated Pan-POTE compared to OSE and FTE cells. Notably, Group 1 & 2 POTEs (POTEs A/B/B2/C/D), Group 3 POTE-actin genes (POTEs E/F/I/J/KP), and other Group 3 POTEs (POTEs G/H/M) show within-group correlated expression, and pan-cancer analyses of tumors and cell lines confirmed this relationship. Based on their restricted expression in normal tissues and increased expression and association with poor prognosis in ovarian cancer, POTEs are potential oncogenes and therapeutic targets in this malignancy.
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spelling pubmed-62443932018-11-28 Expression of the POTE gene family in human ovarian cancer Barger, Carter J Zhang, Wa Sharma, Ashok Chee, Linda James, Smitha R. Kufel, Christina N. Miller, Austin Meza, Jane Drapkin, Ronny Odunsi, Kunle Klinkebiel, David Karpf, Adam R. Sci Rep Article The POTE family includes 14 genes in three phylogenetic groups. We determined POTE mRNA expression in normal tissues, epithelial ovarian and high-grade serous ovarian cancer (EOC, HGSC), and pan-cancer, and determined the relationship of POTE expression to ovarian cancer clinicopathology. Groups 1 & 2 POTEs showed testis-specific expression in normal tissues, consistent with assignment as cancer-testis antigens (CTAs), while Group 3 POTEs were expressed in several normal tissues, indicating they are not CTAs. Pan-POTE and individual POTEs showed significantly elevated expression in EOC and HGSC compared to normal controls. Pan-POTE correlated with increased stage, grade, and the HGSC subtype. Select individual POTEs showed increased expression in recurrent HGSC, and POTEE specifically associated with reduced HGSC OS. Consistent with tumors, EOC cell lines had significantly elevated Pan-POTE compared to OSE and FTE cells. Notably, Group 1 & 2 POTEs (POTEs A/B/B2/C/D), Group 3 POTE-actin genes (POTEs E/F/I/J/KP), and other Group 3 POTEs (POTEs G/H/M) show within-group correlated expression, and pan-cancer analyses of tumors and cell lines confirmed this relationship. Based on their restricted expression in normal tissues and increased expression and association with poor prognosis in ovarian cancer, POTEs are potential oncogenes and therapeutic targets in this malignancy. Nature Publishing Group UK 2018-11-20 /pmc/articles/PMC6244393/ /pubmed/30459449 http://dx.doi.org/10.1038/s41598-018-35567-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Barger, Carter J
Zhang, Wa
Sharma, Ashok
Chee, Linda
James, Smitha R.
Kufel, Christina N.
Miller, Austin
Meza, Jane
Drapkin, Ronny
Odunsi, Kunle
Klinkebiel, David
Karpf, Adam R.
Expression of the POTE gene family in human ovarian cancer
title Expression of the POTE gene family in human ovarian cancer
title_full Expression of the POTE gene family in human ovarian cancer
title_fullStr Expression of the POTE gene family in human ovarian cancer
title_full_unstemmed Expression of the POTE gene family in human ovarian cancer
title_short Expression of the POTE gene family in human ovarian cancer
title_sort expression of the pote gene family in human ovarian cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6244393/
https://www.ncbi.nlm.nih.gov/pubmed/30459449
http://dx.doi.org/10.1038/s41598-018-35567-1
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