Retention-time prediction in comprehensive two-dimensional gas chromatography to aid identification of unknown contaminants

Comprehensive two-dimensional (2D) gas chromatography (GC×GC) coupled to mass spectrometry (MS, GC×GC-MS), which enhances selectivity compared to GC-MS analysis, can be used for non-directed analysis (non-target screening) of environmental samples. Additional tools that aid in identifying unknown compounds are needed to handle the large amount of data generated. These tools include retention indices for characterizing relative retention of compounds and prediction of such. In this study, two quantitative structure–retention relationship (QSRR) approaches for prediction of retention times ((1)t(R) and (2)t(R)) and indices (linear retention indices (LRIs) and a new polyethylene glycol–based retention index (PEG-(2)I)) in GC × GC were explored, and their predictive power compared. In the first method, molecular descriptors combined with partial least squares (PLS) analysis were used to predict times and indices. In the second method, the commercial software package ChromGenius (ACD/Labs), based on a “federation of local models,” was employed. Overall, the PLS approach exhibited better accuracy than the ChromGenius approach. Although average errors for the LRI prediction via ChromGenius were slightly lower, PLS was superior in all other cases. The average deviations between the predicted and the experimental value were 5% and 3% for the (1)t(R) and LRI, and 5% and 12% for the (2)t(R) and PEG-(2)I, respectively. These results are comparable to or better than those reported in previous studies. Finally, the developed model was successfully applied to an independent dataset and led to the discovery of 12 wrongly assigned compounds. The results of the present work represent the first-ever prediction of the PEG-(2)I. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00216-018-1415-x) contains supplementary material, which is available to authorized users.