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An important step towards a prevascularized islet microencapsulation device: in vivo prevascularization by combination of mesenchymal stem cells on micropatterned membranes
Extrahepatic transplantation of islets of Langerhans could aid in better survival of islets after transplantation. When islets are transfused into the liver 60-70% of them are lost immediately after transplantation. An important factor for a successful extrahepatic transplantation is a well-vascular...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6244873/ https://www.ncbi.nlm.nih.gov/pubmed/30413974 http://dx.doi.org/10.1007/s10856-018-6178-6 |
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author | Groot Nibbelink, Milou Skrzypek, Katarzyna Karbaat, Lisanne Both, Sanne Plass, Jacqueline Klomphaar, Bettie van Lente, Jéré Henke, Sieger Karperien, Marcel Stamatialis, Dimitrios van Apeldoorn, Aart |
author_facet | Groot Nibbelink, Milou Skrzypek, Katarzyna Karbaat, Lisanne Both, Sanne Plass, Jacqueline Klomphaar, Bettie van Lente, Jéré Henke, Sieger Karperien, Marcel Stamatialis, Dimitrios van Apeldoorn, Aart |
author_sort | Groot Nibbelink, Milou |
collection | PubMed |
description | Extrahepatic transplantation of islets of Langerhans could aid in better survival of islets after transplantation. When islets are transfused into the liver 60-70% of them are lost immediately after transplantation. An important factor for a successful extrahepatic transplantation is a well-vascularized tissue surrounding the implant. There are many strategies known for enhancing vessel formation such as adding cells with endothelial potential, the combination with angiogenic factors and / or applying surface topography at the exposed surface of the device. Previously we developed porous, micropatterned membranes which can be applied as a lid for an islet encapsulation device and we showed that the surface topography induces human umbilical vein endothelial cell (HUVEC) alignment and interconnection. This was achieved without the addition of hydrogels, often used in angiogenesis assays. In this work, we went one step further towards clinical implementation of the device by combining this micropatterned lid with Mesenchymal Stem Cells (MSCs) to facilitate prevascularization in vivo. As for HUVECs, the micropatterned membranes induced MSC alignment and organization in vitro, an important contributor to vessel formation, whereas in vivo (subcutaneous rat model) they contributed to improved implant prevascularization. In fact, the combination of MSCs seeded on the micropatterned membrane induced the highest vessel formation score in 80% of the sections. [Image: see text] |
format | Online Article Text |
id | pubmed-6244873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-62448732018-12-04 An important step towards a prevascularized islet microencapsulation device: in vivo prevascularization by combination of mesenchymal stem cells on micropatterned membranes Groot Nibbelink, Milou Skrzypek, Katarzyna Karbaat, Lisanne Both, Sanne Plass, Jacqueline Klomphaar, Bettie van Lente, Jéré Henke, Sieger Karperien, Marcel Stamatialis, Dimitrios van Apeldoorn, Aart J Mater Sci Mater Med Special Issue: ESB 2017 Extrahepatic transplantation of islets of Langerhans could aid in better survival of islets after transplantation. When islets are transfused into the liver 60-70% of them are lost immediately after transplantation. An important factor for a successful extrahepatic transplantation is a well-vascularized tissue surrounding the implant. There are many strategies known for enhancing vessel formation such as adding cells with endothelial potential, the combination with angiogenic factors and / or applying surface topography at the exposed surface of the device. Previously we developed porous, micropatterned membranes which can be applied as a lid for an islet encapsulation device and we showed that the surface topography induces human umbilical vein endothelial cell (HUVEC) alignment and interconnection. This was achieved without the addition of hydrogels, often used in angiogenesis assays. In this work, we went one step further towards clinical implementation of the device by combining this micropatterned lid with Mesenchymal Stem Cells (MSCs) to facilitate prevascularization in vivo. As for HUVECs, the micropatterned membranes induced MSC alignment and organization in vitro, an important contributor to vessel formation, whereas in vivo (subcutaneous rat model) they contributed to improved implant prevascularization. In fact, the combination of MSCs seeded on the micropatterned membrane induced the highest vessel formation score in 80% of the sections. [Image: see text] Springer US 2018-11-09 2018 /pmc/articles/PMC6244873/ /pubmed/30413974 http://dx.doi.org/10.1007/s10856-018-6178-6 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, duplication, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Special Issue: ESB 2017 Groot Nibbelink, Milou Skrzypek, Katarzyna Karbaat, Lisanne Both, Sanne Plass, Jacqueline Klomphaar, Bettie van Lente, Jéré Henke, Sieger Karperien, Marcel Stamatialis, Dimitrios van Apeldoorn, Aart An important step towards a prevascularized islet microencapsulation device: in vivo prevascularization by combination of mesenchymal stem cells on micropatterned membranes |
title | An important step towards a prevascularized islet microencapsulation device: in vivo prevascularization by combination of mesenchymal stem cells on micropatterned membranes |
title_full | An important step towards a prevascularized islet microencapsulation device: in vivo prevascularization by combination of mesenchymal stem cells on micropatterned membranes |
title_fullStr | An important step towards a prevascularized islet microencapsulation device: in vivo prevascularization by combination of mesenchymal stem cells on micropatterned membranes |
title_full_unstemmed | An important step towards a prevascularized islet microencapsulation device: in vivo prevascularization by combination of mesenchymal stem cells on micropatterned membranes |
title_short | An important step towards a prevascularized islet microencapsulation device: in vivo prevascularization by combination of mesenchymal stem cells on micropatterned membranes |
title_sort | important step towards a prevascularized islet microencapsulation device: in vivo prevascularization by combination of mesenchymal stem cells on micropatterned membranes |
topic | Special Issue: ESB 2017 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6244873/ https://www.ncbi.nlm.nih.gov/pubmed/30413974 http://dx.doi.org/10.1007/s10856-018-6178-6 |
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