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Leptin receptor is expressed by tissue mast cells

Leptin, the adipose tissue-derived product of the obese (ob) gene, is known to function as the hormone of energy expenditure. It has also been established that leptin regulates immune and inflammatory processes. All leptin-induced biological activities depend on binding to the membrane-spanning lept...

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Autores principales: Żelechowska, Paulina, Wiktorska, Magdalena, Różalska, Sylwia, Stasikowska-Kanicka, Olga, Wągrowska-Danilewicz, Małgorzata, Agier, Justyna, Brzezińska-Błaszczyk, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6244893/
https://www.ncbi.nlm.nih.gov/pubmed/30269202
http://dx.doi.org/10.1007/s12026-018-9029-0
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author Żelechowska, Paulina
Wiktorska, Magdalena
Różalska, Sylwia
Stasikowska-Kanicka, Olga
Wągrowska-Danilewicz, Małgorzata
Agier, Justyna
Brzezińska-Błaszczyk, Ewa
author_facet Żelechowska, Paulina
Wiktorska, Magdalena
Różalska, Sylwia
Stasikowska-Kanicka, Olga
Wągrowska-Danilewicz, Małgorzata
Agier, Justyna
Brzezińska-Błaszczyk, Ewa
author_sort Żelechowska, Paulina
collection PubMed
description Leptin, the adipose tissue-derived product of the obese (ob) gene, is known to function as the hormone of energy expenditure. It has also been established that leptin regulates immune and inflammatory processes. All leptin-induced biological activities depend on binding to the membrane-spanning leptin receptor (Ob-R), belonging to the class I cytokine receptor family. The available data relating to the Ob-R on mature mast cells (MCs), and consequently leptin significance in the modulation of MC activity within the tissue, are limited. Immunohistochemistry was used to establish Ob-R expression by MCs in the mesenteric adipose tissue. Flow cytometry and confocal microscopy were used to evaluate both constitutive and leptin-induced expression of Ob-R on freshly isolated peritoneal MCs. MCs in the mesenteric adipose tissue and native peritoneal MCs express Ob-R constitutively. Additionally, leptin influences its receptor expression on these cells. Leptin at lower concentrations caused Ob-R expression increase both at the cell surface and in the cell interior. MC stimulation with higher concentrations of leptin results in a decline of Ob-R from the cell surface and significant enhancement of this receptor not only in the nuclear region but also in the endoplasmic reticulum. In conclusion, one can be assumed that leptin regulates MC activity within tissues. These findings might provide an additional link among the leptin, innate immune function, and inflammatory processes and diseases.
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spelling pubmed-62448932018-12-04 Leptin receptor is expressed by tissue mast cells Żelechowska, Paulina Wiktorska, Magdalena Różalska, Sylwia Stasikowska-Kanicka, Olga Wągrowska-Danilewicz, Małgorzata Agier, Justyna Brzezińska-Błaszczyk, Ewa Immunol Res Original Article Leptin, the adipose tissue-derived product of the obese (ob) gene, is known to function as the hormone of energy expenditure. It has also been established that leptin regulates immune and inflammatory processes. All leptin-induced biological activities depend on binding to the membrane-spanning leptin receptor (Ob-R), belonging to the class I cytokine receptor family. The available data relating to the Ob-R on mature mast cells (MCs), and consequently leptin significance in the modulation of MC activity within the tissue, are limited. Immunohistochemistry was used to establish Ob-R expression by MCs in the mesenteric adipose tissue. Flow cytometry and confocal microscopy were used to evaluate both constitutive and leptin-induced expression of Ob-R on freshly isolated peritoneal MCs. MCs in the mesenteric adipose tissue and native peritoneal MCs express Ob-R constitutively. Additionally, leptin influences its receptor expression on these cells. Leptin at lower concentrations caused Ob-R expression increase both at the cell surface and in the cell interior. MC stimulation with higher concentrations of leptin results in a decline of Ob-R from the cell surface and significant enhancement of this receptor not only in the nuclear region but also in the endoplasmic reticulum. In conclusion, one can be assumed that leptin regulates MC activity within tissues. These findings might provide an additional link among the leptin, innate immune function, and inflammatory processes and diseases. Springer US 2018-09-29 2018 /pmc/articles/PMC6244893/ /pubmed/30269202 http://dx.doi.org/10.1007/s12026-018-9029-0 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Żelechowska, Paulina
Wiktorska, Magdalena
Różalska, Sylwia
Stasikowska-Kanicka, Olga
Wągrowska-Danilewicz, Małgorzata
Agier, Justyna
Brzezińska-Błaszczyk, Ewa
Leptin receptor is expressed by tissue mast cells
title Leptin receptor is expressed by tissue mast cells
title_full Leptin receptor is expressed by tissue mast cells
title_fullStr Leptin receptor is expressed by tissue mast cells
title_full_unstemmed Leptin receptor is expressed by tissue mast cells
title_short Leptin receptor is expressed by tissue mast cells
title_sort leptin receptor is expressed by tissue mast cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6244893/
https://www.ncbi.nlm.nih.gov/pubmed/30269202
http://dx.doi.org/10.1007/s12026-018-9029-0
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