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An Emulsion System Based on a Chip Polymerase Chain Reaction

In this paper we describe a novel method for detecting many DNA fragments through efficient amplification by using an emulsion system based on “on-chip” PCR instead of conventional multiplex polymerase chain reaction (PCR). During the preparation of on-chip PCR, a set of primers were immobilized on...

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Detalles Bibliográficos
Autores principales: Ge, Qinyu, Yu, Pinfei, Bai, Yunfei, Lu, Zuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245313/
https://www.ncbi.nlm.nih.gov/pubmed/19078848
http://dx.doi.org/10.3390/molecules13123057
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author Ge, Qinyu
Yu, Pinfei
Bai, Yunfei
Lu, Zuhong
author_facet Ge, Qinyu
Yu, Pinfei
Bai, Yunfei
Lu, Zuhong
author_sort Ge, Qinyu
collection PubMed
description In this paper we describe a novel method for detecting many DNA fragments through efficient amplification by using an emulsion system based on “on-chip” PCR instead of conventional multiplex polymerase chain reaction (PCR). During the preparation of on-chip PCR, a set of primers were immobilized on a slide and other sets were in an emulsion system. Different emulsion phase primers and other related PCR components were dispersed in different droplets of the emulsion system, and then, due to the thermal instability of emulsion droplets, they would be released onto the surface of the slide after preheating in the first PCR step. To test the above method, we used plasma DNAs from pregnant women who was carrying a male fetus for gender identification. Four different Y chromosome DNA fragments were selected. Results showed that different DNA fragments could be simultaneously amplified with satisfactory results. It is suggested that a simple, convenient and inexpensive on-chip PCR method has been developed.
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spelling pubmed-62453132018-11-26 An Emulsion System Based on a Chip Polymerase Chain Reaction Ge, Qinyu Yu, Pinfei Bai, Yunfei Lu, Zuhong Molecules Article In this paper we describe a novel method for detecting many DNA fragments through efficient amplification by using an emulsion system based on “on-chip” PCR instead of conventional multiplex polymerase chain reaction (PCR). During the preparation of on-chip PCR, a set of primers were immobilized on a slide and other sets were in an emulsion system. Different emulsion phase primers and other related PCR components were dispersed in different droplets of the emulsion system, and then, due to the thermal instability of emulsion droplets, they would be released onto the surface of the slide after preheating in the first PCR step. To test the above method, we used plasma DNAs from pregnant women who was carrying a male fetus for gender identification. Four different Y chromosome DNA fragments were selected. Results showed that different DNA fragments could be simultaneously amplified with satisfactory results. It is suggested that a simple, convenient and inexpensive on-chip PCR method has been developed. MDPI 2008-12-09 /pmc/articles/PMC6245313/ /pubmed/19078848 http://dx.doi.org/10.3390/molecules13123057 Text en © 2008 by the authors. Licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Ge, Qinyu
Yu, Pinfei
Bai, Yunfei
Lu, Zuhong
An Emulsion System Based on a Chip Polymerase Chain Reaction
title An Emulsion System Based on a Chip Polymerase Chain Reaction
title_full An Emulsion System Based on a Chip Polymerase Chain Reaction
title_fullStr An Emulsion System Based on a Chip Polymerase Chain Reaction
title_full_unstemmed An Emulsion System Based on a Chip Polymerase Chain Reaction
title_short An Emulsion System Based on a Chip Polymerase Chain Reaction
title_sort emulsion system based on a chip polymerase chain reaction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245313/
https://www.ncbi.nlm.nih.gov/pubmed/19078848
http://dx.doi.org/10.3390/molecules13123057
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