Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry

A novel method that uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to analyze molecular weight and sequencing of glucan in Ganoderma lucidum is presented. Thus, β-glucan, which was isolated from fruiting bodies of G. lucidum, was measured in a direct and fast way using MA...

Descripción completa

Detalles Bibliográficos
Autores principales: Hung, Wei-Ting, Wang, Shwu-Huey, Chen, Chung-Hsuan, Yang, Wen-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245394/
https://www.ncbi.nlm.nih.gov/pubmed/18794771
http://dx.doi.org/10.3390/molecules13081538
_version_ 1783372229251170304
author Hung, Wei-Ting
Wang, Shwu-Huey
Chen, Chung-Hsuan
Yang, Wen-Bin
author_facet Hung, Wei-Ting
Wang, Shwu-Huey
Chen, Chung-Hsuan
Yang, Wen-Bin
author_sort Hung, Wei-Ting
collection PubMed
description A novel method that uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to analyze molecular weight and sequencing of glucan in Ganoderma lucidum is presented. Thus, β-glucan, which was isolated from fruiting bodies of G. lucidum, was measured in a direct and fast way using MALDI mass spectrometry. In addition, tandem mass spectrometry of permethylated glucans of G. lucidum, dextran, curdlan and maltohexaose were also pursued and different fragment patterns were obtained. The G. lucidum glucan structure was determined and this method for linkage analysis of permethylated glucan has been proven feasible.
format Online
Article
Text
id pubmed-6245394
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62453942018-11-26 Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry Hung, Wei-Ting Wang, Shwu-Huey Chen, Chung-Hsuan Yang, Wen-Bin Molecules Article A novel method that uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to analyze molecular weight and sequencing of glucan in Ganoderma lucidum is presented. Thus, β-glucan, which was isolated from fruiting bodies of G. lucidum, was measured in a direct and fast way using MALDI mass spectrometry. In addition, tandem mass spectrometry of permethylated glucans of G. lucidum, dextran, curdlan and maltohexaose were also pursued and different fragment patterns were obtained. The G. lucidum glucan structure was determined and this method for linkage analysis of permethylated glucan has been proven feasible. MDPI 2008-08-03 /pmc/articles/PMC6245394/ /pubmed/18794771 http://dx.doi.org/10.3390/molecules13081538 Text en © 2008 by the authors. Licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Hung, Wei-Ting
Wang, Shwu-Huey
Chen, Chung-Hsuan
Yang, Wen-Bin
Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry
title Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry
title_full Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry
title_fullStr Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry
title_full_unstemmed Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry
title_short Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry
title_sort structure determination of β-glucans from ganoderma lucidum with matrix-assisted laser desorption/ionization (maldi) mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245394/
https://www.ncbi.nlm.nih.gov/pubmed/18794771
http://dx.doi.org/10.3390/molecules13081538
work_keys_str_mv AT hungweiting structuredeterminationofbglucansfromganodermalucidumwithmatrixassistedlaserdesorptionionizationmaldimassspectrometry
AT wangshwuhuey structuredeterminationofbglucansfromganodermalucidumwithmatrixassistedlaserdesorptionionizationmaldimassspectrometry
AT chenchunghsuan structuredeterminationofbglucansfromganodermalucidumwithmatrixassistedlaserdesorptionionizationmaldimassspectrometry
AT yangwenbin structuredeterminationofbglucansfromganodermalucidumwithmatrixassistedlaserdesorptionionizationmaldimassspectrometry

Ejemplares similares