Automated sequential chromogenic IHC double staining with two HRP substrates

Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the f...

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Detalles Bibliográficos
Autores principales: Petersen, Kenneth Heesche, Lohse, Jesper, Ramsgaard, Lasse
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245840/
https://www.ncbi.nlm.nih.gov/pubmed/30458050
http://dx.doi.org/10.1371/journal.pone.0207867
Descripción
Sumario:Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining.

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