Automated sequential chromogenic IHC double staining with two HRP substrates

Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the f...

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Detalles Bibliográficos
Autores principales: Petersen, Kenneth Heesche, Lohse, Jesper, Ramsgaard, Lasse
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245840/
https://www.ncbi.nlm.nih.gov/pubmed/30458050
http://dx.doi.org/10.1371/journal.pone.0207867
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author Petersen, Kenneth Heesche
Lohse, Jesper
Ramsgaard, Lasse
author_facet Petersen, Kenneth Heesche
Lohse, Jesper
Ramsgaard, Lasse
author_sort Petersen, Kenneth Heesche
collection PubMed
description Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining.
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spelling pubmed-62458402018-11-30 Automated sequential chromogenic IHC double staining with two HRP substrates Petersen, Kenneth Heesche Lohse, Jesper Ramsgaard, Lasse PLoS One Research Article Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining. Public Library of Science 2018-11-20 /pmc/articles/PMC6245840/ /pubmed/30458050 http://dx.doi.org/10.1371/journal.pone.0207867 Text en © 2018 Petersen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Petersen, Kenneth Heesche
Lohse, Jesper
Ramsgaard, Lasse
Automated sequential chromogenic IHC double staining with two HRP substrates
title Automated sequential chromogenic IHC double staining with two HRP substrates
title_full Automated sequential chromogenic IHC double staining with two HRP substrates
title_fullStr Automated sequential chromogenic IHC double staining with two HRP substrates
title_full_unstemmed Automated sequential chromogenic IHC double staining with two HRP substrates
title_short Automated sequential chromogenic IHC double staining with two HRP substrates
title_sort automated sequential chromogenic ihc double staining with two hrp substrates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245840/
https://www.ncbi.nlm.nih.gov/pubmed/30458050
http://dx.doi.org/10.1371/journal.pone.0207867
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AT ramsgaardlasse automatedsequentialchromogenicihcdoublestainingwithtwohrpsubstrates

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