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Automated sequential chromogenic IHC double staining with two HRP substrates

Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the f...

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Detalles Bibliográficos
Autores principales:	Petersen, Kenneth Heesche, Lohse, Jesper, Ramsgaard, Lasse
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245840/ https://www.ncbi.nlm.nih.gov/pubmed/30458050 http://dx.doi.org/10.1371/journal.pone.0207867

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