Cargando…
Early serum miR-1297 is an indicator of poor neurological outcome in patients with aSAH
Objective: MiRNAs are important regulators of translation and have been described as biomarkers of a number of cardiovascular diseases, including stroke. The purpose of the study was to determine expression levels of serum miR-1297 in patients with aneurysmal subarachnoid hemorrhage (aSAH), and to a...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6246762/ https://www.ncbi.nlm.nih.gov/pubmed/30355655 http://dx.doi.org/10.1042/BSR20180646 |
_version_ | 1783372388164960256 |
---|---|
author | Sheng, Bin Lai, Nian-sheng Yao, Yang Dong, Jin Li, Zhen-bao Zhao, Xin-tong Liu, Jia-qiang Li, Xue-qin Fang, Xing-gen |
author_facet | Sheng, Bin Lai, Nian-sheng Yao, Yang Dong, Jin Li, Zhen-bao Zhao, Xin-tong Liu, Jia-qiang Li, Xue-qin Fang, Xing-gen |
author_sort | Sheng, Bin |
collection | PubMed |
description | Objective: MiRNAs are important regulators of translation and have been described as biomarkers of a number of cardiovascular diseases, including stroke. The purpose of the study was to determine expression levels of serum miR-1297 in patients with aneurysmal subarachnoid hemorrhage (aSAH), and to assess whether miR-1297 was the prognostic indicator of aSAH. Methods: We treated 128 aSAH patients with endovascular coiling. The World Federation of Neurological Surgeons (WFNS) grades, Hunt–Hess grades, and modified Fisher scores were used to assess aSAH severity. Neurologic outcome was assessed using the Modified Rankin Scale (mRS) at 1-year post-aSAH. Serum was taken at various time points (24, 72, and 168 h, and 14 days). Serum samples from aSAH patients and healthy controls were subjected to reverse transcription (RT) quantitative real-time PCR (RT-qPCR). Results: A poor outcome at 1 year was associated with significantly higher levels of miR-1297 value at the four time points, higher WFNS grade, higher Hunt–Hess grade, and higher Fisher score. Serum miR-1297 levels were significantly higher in patients, compared with healthy controls. There were significant correlations of miR-1297 concentrations in serum with severity in aSAH. The AUCs of miR-1297 at the four time points for distinguishing the aSAH patients from healthy controls were 0.80, 0.94, 0.77, and 0.59, respectively. After multivariate logistic regression analysis, only miR-1297 at 24 and 72 h enabled prediction of neurological outcome at 1 year. Conclusion: Serum was an independent predictive factor of poor outcome at 1 year following aSAH. This result supports the use of miR-1297 in aSAH to aid determination of prognosis. |
format | Online Article Text |
id | pubmed-6246762 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62467622018-11-28 Early serum miR-1297 is an indicator of poor neurological outcome in patients with aSAH Sheng, Bin Lai, Nian-sheng Yao, Yang Dong, Jin Li, Zhen-bao Zhao, Xin-tong Liu, Jia-qiang Li, Xue-qin Fang, Xing-gen Biosci Rep Research Articles Objective: MiRNAs are important regulators of translation and have been described as biomarkers of a number of cardiovascular diseases, including stroke. The purpose of the study was to determine expression levels of serum miR-1297 in patients with aneurysmal subarachnoid hemorrhage (aSAH), and to assess whether miR-1297 was the prognostic indicator of aSAH. Methods: We treated 128 aSAH patients with endovascular coiling. The World Federation of Neurological Surgeons (WFNS) grades, Hunt–Hess grades, and modified Fisher scores were used to assess aSAH severity. Neurologic outcome was assessed using the Modified Rankin Scale (mRS) at 1-year post-aSAH. Serum was taken at various time points (24, 72, and 168 h, and 14 days). Serum samples from aSAH patients and healthy controls were subjected to reverse transcription (RT) quantitative real-time PCR (RT-qPCR). Results: A poor outcome at 1 year was associated with significantly higher levels of miR-1297 value at the four time points, higher WFNS grade, higher Hunt–Hess grade, and higher Fisher score. Serum miR-1297 levels were significantly higher in patients, compared with healthy controls. There were significant correlations of miR-1297 concentrations in serum with severity in aSAH. The AUCs of miR-1297 at the four time points for distinguishing the aSAH patients from healthy controls were 0.80, 0.94, 0.77, and 0.59, respectively. After multivariate logistic regression analysis, only miR-1297 at 24 and 72 h enabled prediction of neurological outcome at 1 year. Conclusion: Serum was an independent predictive factor of poor outcome at 1 year following aSAH. This result supports the use of miR-1297 in aSAH to aid determination of prognosis. Portland Press Ltd. 2018-11-21 /pmc/articles/PMC6246762/ /pubmed/30355655 http://dx.doi.org/10.1042/BSR20180646 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Sheng, Bin Lai, Nian-sheng Yao, Yang Dong, Jin Li, Zhen-bao Zhao, Xin-tong Liu, Jia-qiang Li, Xue-qin Fang, Xing-gen Early serum miR-1297 is an indicator of poor neurological outcome in patients with aSAH |
title | Early serum miR-1297 is an indicator of poor neurological outcome in patients with aSAH |
title_full | Early serum miR-1297 is an indicator of poor neurological outcome in patients with aSAH |
title_fullStr | Early serum miR-1297 is an indicator of poor neurological outcome in patients with aSAH |
title_full_unstemmed | Early serum miR-1297 is an indicator of poor neurological outcome in patients with aSAH |
title_short | Early serum miR-1297 is an indicator of poor neurological outcome in patients with aSAH |
title_sort | early serum mir-1297 is an indicator of poor neurological outcome in patients with asah |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6246762/ https://www.ncbi.nlm.nih.gov/pubmed/30355655 http://dx.doi.org/10.1042/BSR20180646 |
work_keys_str_mv | AT shengbin earlyserummir1297isanindicatorofpoorneurologicaloutcomeinpatientswithasah AT lainiansheng earlyserummir1297isanindicatorofpoorneurologicaloutcomeinpatientswithasah AT yaoyang earlyserummir1297isanindicatorofpoorneurologicaloutcomeinpatientswithasah AT dongjin earlyserummir1297isanindicatorofpoorneurologicaloutcomeinpatientswithasah AT lizhenbao earlyserummir1297isanindicatorofpoorneurologicaloutcomeinpatientswithasah AT zhaoxintong earlyserummir1297isanindicatorofpoorneurologicaloutcomeinpatientswithasah AT liujiaqiang earlyserummir1297isanindicatorofpoorneurologicaloutcomeinpatientswithasah AT lixueqin earlyserummir1297isanindicatorofpoorneurologicaloutcomeinpatientswithasah AT fangxinggen earlyserummir1297isanindicatorofpoorneurologicaloutcomeinpatientswithasah |