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No difference in human mast cells derived from peanut allergic versus non‐allergic subjects
INTRODUCTION: Mast cells are the primary effector cells of allergy. This study aimed at characterizing human peripheral blood‐derived mast cells (PBdMC) from peanut allergic and non‐allergic subjects by investigating whether the molecular and stimulus‐response profile of PBdMC discriminate between p...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247235/ https://www.ncbi.nlm.nih.gov/pubmed/29992767 http://dx.doi.org/10.1002/iid3.226 |
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author | Larsen, Lau F. Juel‐Berg, Nanna Hansen, Anker Hansen, Kirsten S. Mills, E. N. Clare van Ree, Ronald Rådinger, Madeleine Poulsen, Lars K. Jensen, Bettina M. |
author_facet | Larsen, Lau F. Juel‐Berg, Nanna Hansen, Anker Hansen, Kirsten S. Mills, E. N. Clare van Ree, Ronald Rådinger, Madeleine Poulsen, Lars K. Jensen, Bettina M. |
author_sort | Larsen, Lau F. |
collection | PubMed |
description | INTRODUCTION: Mast cells are the primary effector cells of allergy. This study aimed at characterizing human peripheral blood‐derived mast cells (PBdMC) from peanut allergic and non‐allergic subjects by investigating whether the molecular and stimulus‐response profile of PBdMC discriminate between peanut allergic and healthy individuals. METHODS: PBdMC were generated from eight peanut allergic and 10 non‐allergic subjects. The molecular profile (cell surface receptor expression) was assessed using flow cytometry. The stimulus‐response profile (histamine release induced by secretagogues, secretion of cytokines/chemokines and changes in miRNA expression following anti‐IgE activation) was carried out with histamine release test, luminex multiplex assay and miRNA arrays. RESULTS: Expression of activating receptors (FcϵRI, CD48, CD88, CD117, and C3aR) on PBdMC was not different among peanut allergic and non‐allergic subjects. Likewise, inhibitory receptors (CD32, CD200R, CD300a, and siglec‐8) displayed comparable levels of expression. Both groups of PBdMC were unresponsive to substance P, compound 48/80 and C5a but released comparable levels of histamine when stimulated with anti‐IgE and C3a. Interestingly, among the secreted cytokines/chemokines (IL‐8, IL‐10, IL‐13, IL‐23, IL‐31, IL‐37, MCP‐1, VEGF, GM‐CSF) PBdMC from peanut allergic subjects showed a different secretion pattern of IL‐31 compared to non‐allergic subjects. Investigating miRNA expression from resting or activated PBdMC revealed no significantly difference between peanut allergic and non‐allergic subjects. CONCLUSION: The molecular and stimulus‐response profile revealed that PBdMC from peanut allergic subjects differently express IL‐31 compared to non‐allergic subjects. However, since only one altered parameter was found among 893 investigated, it is still questionable if the pathophysiological mechanisms of peanut allergy are revealed in PBdMC. |
format | Online Article Text |
id | pubmed-6247235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62472352018-11-26 No difference in human mast cells derived from peanut allergic versus non‐allergic subjects Larsen, Lau F. Juel‐Berg, Nanna Hansen, Anker Hansen, Kirsten S. Mills, E. N. Clare van Ree, Ronald Rådinger, Madeleine Poulsen, Lars K. Jensen, Bettina M. Immun Inflamm Dis Original Research INTRODUCTION: Mast cells are the primary effector cells of allergy. This study aimed at characterizing human peripheral blood‐derived mast cells (PBdMC) from peanut allergic and non‐allergic subjects by investigating whether the molecular and stimulus‐response profile of PBdMC discriminate between peanut allergic and healthy individuals. METHODS: PBdMC were generated from eight peanut allergic and 10 non‐allergic subjects. The molecular profile (cell surface receptor expression) was assessed using flow cytometry. The stimulus‐response profile (histamine release induced by secretagogues, secretion of cytokines/chemokines and changes in miRNA expression following anti‐IgE activation) was carried out with histamine release test, luminex multiplex assay and miRNA arrays. RESULTS: Expression of activating receptors (FcϵRI, CD48, CD88, CD117, and C3aR) on PBdMC was not different among peanut allergic and non‐allergic subjects. Likewise, inhibitory receptors (CD32, CD200R, CD300a, and siglec‐8) displayed comparable levels of expression. Both groups of PBdMC were unresponsive to substance P, compound 48/80 and C5a but released comparable levels of histamine when stimulated with anti‐IgE and C3a. Interestingly, among the secreted cytokines/chemokines (IL‐8, IL‐10, IL‐13, IL‐23, IL‐31, IL‐37, MCP‐1, VEGF, GM‐CSF) PBdMC from peanut allergic subjects showed a different secretion pattern of IL‐31 compared to non‐allergic subjects. Investigating miRNA expression from resting or activated PBdMC revealed no significantly difference between peanut allergic and non‐allergic subjects. CONCLUSION: The molecular and stimulus‐response profile revealed that PBdMC from peanut allergic subjects differently express IL‐31 compared to non‐allergic subjects. However, since only one altered parameter was found among 893 investigated, it is still questionable if the pathophysiological mechanisms of peanut allergy are revealed in PBdMC. John Wiley and Sons Inc. 2018-07-10 /pmc/articles/PMC6247235/ /pubmed/29992767 http://dx.doi.org/10.1002/iid3.226 Text en © 2018 The Authors. Immunity, Inflammation and Disease Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Larsen, Lau F. Juel‐Berg, Nanna Hansen, Anker Hansen, Kirsten S. Mills, E. N. Clare van Ree, Ronald Rådinger, Madeleine Poulsen, Lars K. Jensen, Bettina M. No difference in human mast cells derived from peanut allergic versus non‐allergic subjects |
title | No difference in human mast cells derived from peanut allergic versus non‐allergic subjects |
title_full | No difference in human mast cells derived from peanut allergic versus non‐allergic subjects |
title_fullStr | No difference in human mast cells derived from peanut allergic versus non‐allergic subjects |
title_full_unstemmed | No difference in human mast cells derived from peanut allergic versus non‐allergic subjects |
title_short | No difference in human mast cells derived from peanut allergic versus non‐allergic subjects |
title_sort | no difference in human mast cells derived from peanut allergic versus non‐allergic subjects |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247235/ https://www.ncbi.nlm.nih.gov/pubmed/29992767 http://dx.doi.org/10.1002/iid3.226 |
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