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Morphological and molecular datasets for Kaempferia species

This study compared morphological and molecular data for identification of Kaempferia species. Each species was deposited in Institute of Bioscience (IBS), Universiti Putra Malaysia (UPM) as voucher specimens and ITS sequences of each species deposited in NCBI (https://www.ncbi.nlm.nih.gov/) as GenB...

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Detalles Bibliográficos
Autores principales: Labrooy, Catherine, Abdullah, Thohirah Lee, Stanslas, Johnson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247442/
https://www.ncbi.nlm.nih.gov/pubmed/30505900
http://dx.doi.org/10.1016/j.dib.2018.10.097
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author Labrooy, Catherine
Abdullah, Thohirah Lee
Stanslas, Johnson
author_facet Labrooy, Catherine
Abdullah, Thohirah Lee
Stanslas, Johnson
author_sort Labrooy, Catherine
collection PubMed
description This study compared morphological and molecular data for identification of Kaempferia species. Each species was deposited in Institute of Bioscience (IBS), Universiti Putra Malaysia (UPM) as voucher specimens and ITS sequences of each species deposited in NCBI (https://www.ncbi.nlm.nih.gov/) as GenBank accessions. DNA was extracted using a modified CTAB method and PCR amplification was completed using Internal Transcribed Spacer (ITS4 and ITS5) markers. PCR amplification of products were viewed under gel electrophoresis. Sequencing was performed and sequence characteristics of ITS rDNA in Kaempferia is shown. Qualitative and qualitative scoring of morphological characters and measuring techniques for Kaempferia species are included. In addition, a brief review of molecular markers used in phylogenetic studies of Zingiberaceae is included in this dataset.
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spelling pubmed-62474422018-11-30 Morphological and molecular datasets for Kaempferia species Labrooy, Catherine Abdullah, Thohirah Lee Stanslas, Johnson Data Brief Agricultural and Biological Science This study compared morphological and molecular data for identification of Kaempferia species. Each species was deposited in Institute of Bioscience (IBS), Universiti Putra Malaysia (UPM) as voucher specimens and ITS sequences of each species deposited in NCBI (https://www.ncbi.nlm.nih.gov/) as GenBank accessions. DNA was extracted using a modified CTAB method and PCR amplification was completed using Internal Transcribed Spacer (ITS4 and ITS5) markers. PCR amplification of products were viewed under gel electrophoresis. Sequencing was performed and sequence characteristics of ITS rDNA in Kaempferia is shown. Qualitative and qualitative scoring of morphological characters and measuring techniques for Kaempferia species are included. In addition, a brief review of molecular markers used in phylogenetic studies of Zingiberaceae is included in this dataset. Elsevier 2018-10-27 /pmc/articles/PMC6247442/ /pubmed/30505900 http://dx.doi.org/10.1016/j.dib.2018.10.097 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Agricultural and Biological Science
Labrooy, Catherine
Abdullah, Thohirah Lee
Stanslas, Johnson
Morphological and molecular datasets for Kaempferia species
title Morphological and molecular datasets for Kaempferia species
title_full Morphological and molecular datasets for Kaempferia species
title_fullStr Morphological and molecular datasets for Kaempferia species
title_full_unstemmed Morphological and molecular datasets for Kaempferia species
title_short Morphological and molecular datasets for Kaempferia species
title_sort morphological and molecular datasets for kaempferia species
topic Agricultural and Biological Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247442/
https://www.ncbi.nlm.nih.gov/pubmed/30505900
http://dx.doi.org/10.1016/j.dib.2018.10.097
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