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Morphological and molecular datasets for Kaempferia species
This study compared morphological and molecular data for identification of Kaempferia species. Each species was deposited in Institute of Bioscience (IBS), Universiti Putra Malaysia (UPM) as voucher specimens and ITS sequences of each species deposited in NCBI (https://www.ncbi.nlm.nih.gov/) as GenB...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247442/ https://www.ncbi.nlm.nih.gov/pubmed/30505900 http://dx.doi.org/10.1016/j.dib.2018.10.097 |
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author | Labrooy, Catherine Abdullah, Thohirah Lee Stanslas, Johnson |
author_facet | Labrooy, Catherine Abdullah, Thohirah Lee Stanslas, Johnson |
author_sort | Labrooy, Catherine |
collection | PubMed |
description | This study compared morphological and molecular data for identification of Kaempferia species. Each species was deposited in Institute of Bioscience (IBS), Universiti Putra Malaysia (UPM) as voucher specimens and ITS sequences of each species deposited in NCBI (https://www.ncbi.nlm.nih.gov/) as GenBank accessions. DNA was extracted using a modified CTAB method and PCR amplification was completed using Internal Transcribed Spacer (ITS4 and ITS5) markers. PCR amplification of products were viewed under gel electrophoresis. Sequencing was performed and sequence characteristics of ITS rDNA in Kaempferia is shown. Qualitative and qualitative scoring of morphological characters and measuring techniques for Kaempferia species are included. In addition, a brief review of molecular markers used in phylogenetic studies of Zingiberaceae is included in this dataset. |
format | Online Article Text |
id | pubmed-6247442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-62474422018-11-30 Morphological and molecular datasets for Kaempferia species Labrooy, Catherine Abdullah, Thohirah Lee Stanslas, Johnson Data Brief Agricultural and Biological Science This study compared morphological and molecular data for identification of Kaempferia species. Each species was deposited in Institute of Bioscience (IBS), Universiti Putra Malaysia (UPM) as voucher specimens and ITS sequences of each species deposited in NCBI (https://www.ncbi.nlm.nih.gov/) as GenBank accessions. DNA was extracted using a modified CTAB method and PCR amplification was completed using Internal Transcribed Spacer (ITS4 and ITS5) markers. PCR amplification of products were viewed under gel electrophoresis. Sequencing was performed and sequence characteristics of ITS rDNA in Kaempferia is shown. Qualitative and qualitative scoring of morphological characters and measuring techniques for Kaempferia species are included. In addition, a brief review of molecular markers used in phylogenetic studies of Zingiberaceae is included in this dataset. Elsevier 2018-10-27 /pmc/articles/PMC6247442/ /pubmed/30505900 http://dx.doi.org/10.1016/j.dib.2018.10.097 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Agricultural and Biological Science Labrooy, Catherine Abdullah, Thohirah Lee Stanslas, Johnson Morphological and molecular datasets for Kaempferia species |
title | Morphological and molecular datasets for Kaempferia species |
title_full | Morphological and molecular datasets for Kaempferia species |
title_fullStr | Morphological and molecular datasets for Kaempferia species |
title_full_unstemmed | Morphological and molecular datasets for Kaempferia species |
title_short | Morphological and molecular datasets for Kaempferia species |
title_sort | morphological and molecular datasets for kaempferia species |
topic | Agricultural and Biological Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247442/ https://www.ncbi.nlm.nih.gov/pubmed/30505900 http://dx.doi.org/10.1016/j.dib.2018.10.097 |
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