Cargando…
AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening
Using the atomic force microscopy- (AFM-) PeakForce quantitative nanomechanical mapping (QNM) technique, we have previously shown that the adventitia of the human internal mammary artery (IMA), tested under dehydrated conditions, is altered in patients with a high degree of arterial stiffening. In t...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247466/ https://www.ncbi.nlm.nih.gov/pubmed/30524642 http://dx.doi.org/10.1155/2018/6340425 |
_version_ | 1783372479511658496 |
---|---|
author | Chang, Zhuo Paoletti, Paolo Hansen, Maria Lyck Beck, Hans Christian Chen, Po-Yu Rasmussen, Lars Melholt Akhtar, Riaz |
author_facet | Chang, Zhuo Paoletti, Paolo Hansen, Maria Lyck Beck, Hans Christian Chen, Po-Yu Rasmussen, Lars Melholt Akhtar, Riaz |
author_sort | Chang, Zhuo |
collection | PubMed |
description | Using the atomic force microscopy- (AFM-) PeakForce quantitative nanomechanical mapping (QNM) technique, we have previously shown that the adventitia of the human internal mammary artery (IMA), tested under dehydrated conditions, is altered in patients with a high degree of arterial stiffening. In this study, we explored the nanoscale elastic modulus of the tunica media of the IMA in hydrated and dehydrated conditions from the patients with low and high arterial stiffening, as assessed in vivo by carotid-femoral pulse wave velocity (PWV). In both hydrated and dehydrated conditions, the medial layer was significantly stiffer in the high PWV group. The elastic modulus of the hydrated and dehydrated tunica media was significantly correlated with PWV. In the hydrated condition, the expression activity of certain small leucine-rich repeat proteoglycans (SLRPs), which are associated with arterial stiffening, were found to be negatively correlated to the medial elastic modulus. We also compared the data with our previous work on the IMA adventitia. We found that the hydrated media and dehydrated adventitia are both suitable for reflecting the development of arterial stiffening and SLRP expression. This comprehensive study of the nanomechanical properties integrated with the proteomic analysis in the IMAs demonstrates the possibility of linking structural properties and function in small biological samples with novel AFM methods. The IMA is a suitable target for predicting arterial stiffening. |
format | Online Article Text |
id | pubmed-6247466 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-62474662018-12-06 AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening Chang, Zhuo Paoletti, Paolo Hansen, Maria Lyck Beck, Hans Christian Chen, Po-Yu Rasmussen, Lars Melholt Akhtar, Riaz Scanning Research Article Using the atomic force microscopy- (AFM-) PeakForce quantitative nanomechanical mapping (QNM) technique, we have previously shown that the adventitia of the human internal mammary artery (IMA), tested under dehydrated conditions, is altered in patients with a high degree of arterial stiffening. In this study, we explored the nanoscale elastic modulus of the tunica media of the IMA in hydrated and dehydrated conditions from the patients with low and high arterial stiffening, as assessed in vivo by carotid-femoral pulse wave velocity (PWV). In both hydrated and dehydrated conditions, the medial layer was significantly stiffer in the high PWV group. The elastic modulus of the hydrated and dehydrated tunica media was significantly correlated with PWV. In the hydrated condition, the expression activity of certain small leucine-rich repeat proteoglycans (SLRPs), which are associated with arterial stiffening, were found to be negatively correlated to the medial elastic modulus. We also compared the data with our previous work on the IMA adventitia. We found that the hydrated media and dehydrated adventitia are both suitable for reflecting the development of arterial stiffening and SLRP expression. This comprehensive study of the nanomechanical properties integrated with the proteomic analysis in the IMAs demonstrates the possibility of linking structural properties and function in small biological samples with novel AFM methods. The IMA is a suitable target for predicting arterial stiffening. Hindawi 2018-11-05 /pmc/articles/PMC6247466/ /pubmed/30524642 http://dx.doi.org/10.1155/2018/6340425 Text en Copyright © 2018 Zhuo Chang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chang, Zhuo Paoletti, Paolo Hansen, Maria Lyck Beck, Hans Christian Chen, Po-Yu Rasmussen, Lars Melholt Akhtar, Riaz AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening |
title | AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening |
title_full | AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening |
title_fullStr | AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening |
title_full_unstemmed | AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening |
title_short | AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening |
title_sort | afm characterization of the internal mammary artery as a novel target for arterial stiffening |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247466/ https://www.ncbi.nlm.nih.gov/pubmed/30524642 http://dx.doi.org/10.1155/2018/6340425 |
work_keys_str_mv | AT changzhuo afmcharacterizationoftheinternalmammaryarteryasanoveltargetforarterialstiffening AT paolettipaolo afmcharacterizationoftheinternalmammaryarteryasanoveltargetforarterialstiffening AT hansenmarialyck afmcharacterizationoftheinternalmammaryarteryasanoveltargetforarterialstiffening AT beckhanschristian afmcharacterizationoftheinternalmammaryarteryasanoveltargetforarterialstiffening AT chenpoyu afmcharacterizationoftheinternalmammaryarteryasanoveltargetforarterialstiffening AT rasmussenlarsmelholt afmcharacterizationoftheinternalmammaryarteryasanoveltargetforarterialstiffening AT akhtarriaz afmcharacterizationoftheinternalmammaryarteryasanoveltargetforarterialstiffening |