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AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening

Using the atomic force microscopy- (AFM-) PeakForce quantitative nanomechanical mapping (QNM) technique, we have previously shown that the adventitia of the human internal mammary artery (IMA), tested under dehydrated conditions, is altered in patients with a high degree of arterial stiffening. In t...

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Autores principales: Chang, Zhuo, Paoletti, Paolo, Hansen, Maria Lyck, Beck, Hans Christian, Chen, Po-Yu, Rasmussen, Lars Melholt, Akhtar, Riaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247466/
https://www.ncbi.nlm.nih.gov/pubmed/30524642
http://dx.doi.org/10.1155/2018/6340425
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author Chang, Zhuo
Paoletti, Paolo
Hansen, Maria Lyck
Beck, Hans Christian
Chen, Po-Yu
Rasmussen, Lars Melholt
Akhtar, Riaz
author_facet Chang, Zhuo
Paoletti, Paolo
Hansen, Maria Lyck
Beck, Hans Christian
Chen, Po-Yu
Rasmussen, Lars Melholt
Akhtar, Riaz
author_sort Chang, Zhuo
collection PubMed
description Using the atomic force microscopy- (AFM-) PeakForce quantitative nanomechanical mapping (QNM) technique, we have previously shown that the adventitia of the human internal mammary artery (IMA), tested under dehydrated conditions, is altered in patients with a high degree of arterial stiffening. In this study, we explored the nanoscale elastic modulus of the tunica media of the IMA in hydrated and dehydrated conditions from the patients with low and high arterial stiffening, as assessed in vivo by carotid-femoral pulse wave velocity (PWV). In both hydrated and dehydrated conditions, the medial layer was significantly stiffer in the high PWV group. The elastic modulus of the hydrated and dehydrated tunica media was significantly correlated with PWV. In the hydrated condition, the expression activity of certain small leucine-rich repeat proteoglycans (SLRPs), which are associated with arterial stiffening, were found to be negatively correlated to the medial elastic modulus. We also compared the data with our previous work on the IMA adventitia. We found that the hydrated media and dehydrated adventitia are both suitable for reflecting the development of arterial stiffening and SLRP expression. This comprehensive study of the nanomechanical properties integrated with the proteomic analysis in the IMAs demonstrates the possibility of linking structural properties and function in small biological samples with novel AFM methods. The IMA is a suitable target for predicting arterial stiffening.
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spelling pubmed-62474662018-12-06 AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening Chang, Zhuo Paoletti, Paolo Hansen, Maria Lyck Beck, Hans Christian Chen, Po-Yu Rasmussen, Lars Melholt Akhtar, Riaz Scanning Research Article Using the atomic force microscopy- (AFM-) PeakForce quantitative nanomechanical mapping (QNM) technique, we have previously shown that the adventitia of the human internal mammary artery (IMA), tested under dehydrated conditions, is altered in patients with a high degree of arterial stiffening. In this study, we explored the nanoscale elastic modulus of the tunica media of the IMA in hydrated and dehydrated conditions from the patients with low and high arterial stiffening, as assessed in vivo by carotid-femoral pulse wave velocity (PWV). In both hydrated and dehydrated conditions, the medial layer was significantly stiffer in the high PWV group. The elastic modulus of the hydrated and dehydrated tunica media was significantly correlated with PWV. In the hydrated condition, the expression activity of certain small leucine-rich repeat proteoglycans (SLRPs), which are associated with arterial stiffening, were found to be negatively correlated to the medial elastic modulus. We also compared the data with our previous work on the IMA adventitia. We found that the hydrated media and dehydrated adventitia are both suitable for reflecting the development of arterial stiffening and SLRP expression. This comprehensive study of the nanomechanical properties integrated with the proteomic analysis in the IMAs demonstrates the possibility of linking structural properties and function in small biological samples with novel AFM methods. The IMA is a suitable target for predicting arterial stiffening. Hindawi 2018-11-05 /pmc/articles/PMC6247466/ /pubmed/30524642 http://dx.doi.org/10.1155/2018/6340425 Text en Copyright © 2018 Zhuo Chang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chang, Zhuo
Paoletti, Paolo
Hansen, Maria Lyck
Beck, Hans Christian
Chen, Po-Yu
Rasmussen, Lars Melholt
Akhtar, Riaz
AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening
title AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening
title_full AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening
title_fullStr AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening
title_full_unstemmed AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening
title_short AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening
title_sort afm characterization of the internal mammary artery as a novel target for arterial stiffening
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247466/
https://www.ncbi.nlm.nih.gov/pubmed/30524642
http://dx.doi.org/10.1155/2018/6340425
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