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Mouse mast cells and mast cell proteases do not play a significant role in acute tissue injury pain induced by formalin
Subcutaneous formalin injections are used as a model for tissue injury-induced pain where formalin induces pain and inflammation indirectly by crosslinking proteins and directly through activation of the transient receptor potential A1 receptor on primary afferents. Activation of primary afferents l...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247485/ https://www.ncbi.nlm.nih.gov/pubmed/30280636 http://dx.doi.org/10.1177/1744806918808161 |
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author | Magnúsdóttir, Elín I Grujic, Mirjana Roers, Axel Hartmann, Karin Pejler, Gunnar Lagerström, Malin C |
author_facet | Magnúsdóttir, Elín I Grujic, Mirjana Roers, Axel Hartmann, Karin Pejler, Gunnar Lagerström, Malin C |
author_sort | Magnúsdóttir, Elín I |
collection | PubMed |
description | Subcutaneous formalin injections are used as a model for tissue injury-induced pain where formalin induces pain and inflammation indirectly by crosslinking proteins and directly through activation of the transient receptor potential A1 receptor on primary afferents. Activation of primary afferents leads to both central and peripheral release of neurotransmitters. Mast cells are found in close proximity to peripheral sensory nerve endings and express receptors for neurotransmitters released by the primary afferents, contributing to the neuro/immune interface. Mast cell proteases are found in large quantities within mast cell granules and are released continuously in small amounts and upon mast cell activation. They have a wide repertoire of proposed substrates, including Substance P and calcitonin gene-related peptide, but knowledge of their in vivo function is limited. We evaluated the role of mouse mast cell proteases (mMCPs) in tissue injury pain responses induced by formalin, using transgenic mice lacking either mMCP4, mMCP6, or carboxypeptidase A3 (CPA3), or mast cells in their entirety. Further, we investigated the role of mast cells in heat hypersensitivity following a nerve growth factor injection. No statistical difference was observed between the respective mast cell protease knockout lines and wild-type controls in the formalin test. Mast cell deficiency did not have an effect on formalin-induced nociceptive responses nor nerve growth factor-induced heat hypersensitivity. Our data thus show that mMCP4, mMCP6, and CPA3 as well as mast cells as a whole, do not play a significant role in the pain responses associated with acute tissue injury and inflammation in the formalin test. Our data also indicate that mast cells are not essential to heat hypersensitivity induced by nerve growth factor. |
format | Online Article Text |
id | pubmed-6247485 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-62474852018-11-26 Mouse mast cells and mast cell proteases do not play a significant role in acute tissue injury pain induced by formalin Magnúsdóttir, Elín I Grujic, Mirjana Roers, Axel Hartmann, Karin Pejler, Gunnar Lagerström, Malin C Mol Pain Short Report Subcutaneous formalin injections are used as a model for tissue injury-induced pain where formalin induces pain and inflammation indirectly by crosslinking proteins and directly through activation of the transient receptor potential A1 receptor on primary afferents. Activation of primary afferents leads to both central and peripheral release of neurotransmitters. Mast cells are found in close proximity to peripheral sensory nerve endings and express receptors for neurotransmitters released by the primary afferents, contributing to the neuro/immune interface. Mast cell proteases are found in large quantities within mast cell granules and are released continuously in small amounts and upon mast cell activation. They have a wide repertoire of proposed substrates, including Substance P and calcitonin gene-related peptide, but knowledge of their in vivo function is limited. We evaluated the role of mouse mast cell proteases (mMCPs) in tissue injury pain responses induced by formalin, using transgenic mice lacking either mMCP4, mMCP6, or carboxypeptidase A3 (CPA3), or mast cells in their entirety. Further, we investigated the role of mast cells in heat hypersensitivity following a nerve growth factor injection. No statistical difference was observed between the respective mast cell protease knockout lines and wild-type controls in the formalin test. Mast cell deficiency did not have an effect on formalin-induced nociceptive responses nor nerve growth factor-induced heat hypersensitivity. Our data thus show that mMCP4, mMCP6, and CPA3 as well as mast cells as a whole, do not play a significant role in the pain responses associated with acute tissue injury and inflammation in the formalin test. Our data also indicate that mast cells are not essential to heat hypersensitivity induced by nerve growth factor. SAGE Publications 2018-10-03 /pmc/articles/PMC6247485/ /pubmed/30280636 http://dx.doi.org/10.1177/1744806918808161 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Short Report Magnúsdóttir, Elín I Grujic, Mirjana Roers, Axel Hartmann, Karin Pejler, Gunnar Lagerström, Malin C Mouse mast cells and mast cell proteases do not play a significant role in acute tissue injury pain induced by formalin |
title | Mouse mast cells and mast cell proteases do not play a significant role in acute tissue injury pain induced by formalin |
title_full | Mouse mast cells and mast cell proteases do not play a significant role in acute tissue injury pain induced by formalin |
title_fullStr | Mouse mast cells and mast cell proteases do not play a significant role in acute tissue injury pain induced by formalin |
title_full_unstemmed | Mouse mast cells and mast cell proteases do not play a significant role in acute tissue injury pain induced by formalin |
title_short | Mouse mast cells and mast cell proteases do not play a significant role in acute tissue injury pain induced by formalin |
title_sort | mouse mast cells and mast cell proteases do not play a significant role in acute tissue injury pain induced by formalin |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247485/ https://www.ncbi.nlm.nih.gov/pubmed/30280636 http://dx.doi.org/10.1177/1744806918808161 |
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