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Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay

Porcine deltacoronavirus (PDCoV) is a newly discovered coronavirus, which belongs to the family Coronaviridae. It causes watery diarrhea, vomiting, and dehydration in newborn piglets. A sensitive RT-PCR method is urgently required to detect PDCoV infection. In this study, we developed and evaluated...

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Detalles Bibliográficos
Autores principales: Ma, Lei, Zeng, Fanwen, Huang, Bihong, Cong, Feng, Huang, Ren, Ma, Jingyun, Guo, Pengju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247729/
https://www.ncbi.nlm.nih.gov/pubmed/30533434
http://dx.doi.org/10.1155/2018/5035139