Comparison of Landmark- and Outline-Based Geometric Morphometrics for Discriminating Mosquito Vectors in Ratchaburi Province, Thailand

It is often challenging to identify mosquito vectors in the field based on morphological features due to their similar morphologies and difficulties in obtaining undamaged samples but is required for their successful control. Geometric morphometrics (GM) overcomes this issue by analyzing a suite of traits simultaneously and has the added advantages of being easy to use, low cost, and quick. Therefore, this research compared the efficiency and precision of landmark- and outline-based GM techniques for separating species of mosquitoes in Huay Nam Nak village, Ratchaburi Province, Thailand. This research collected 273 individuals belonging to seven species: Anopheles barbirostris, An. subpictus, Culex quinquefasciatus, Cx. vishnui, Cx. whitmorei, Aedes aegypti, and Ae. albopictus. Both landmark-based and outline-based GM techniques could identify malaria vectors in this area to the genus level successfully and were also very effective for identifying the malaria vectors Anopheles spp. and the dengue vectors Aedes spp. to the species level. However, they were less effective for distinguishing between species of Culex. Therefore, GM represents a valuable tool for the identification of mosquito vectors in the field, which will facilitate their successful control.