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Effect of the combined action of Quercus cortex extract and probiotic substances on the immunity and productivity of broiler chickens

AIM: This study was designed to investigate the synergistic effect of the combined action of probiotic bacterial strains (Bifidobacterium adolescentis and Lactobacillus acidophilus) and Quercus cortex extract as biologically active substances in the feed on the immunity and productivity of Gallus ga...

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Autores principales: Duskaev, G. K., Rakhmatullin, S. G., Kazachkova, N. M., Sheida, Y. V., Mikolaychik, I. N., Morozova, L. A., Galiev, B. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247875/
https://www.ncbi.nlm.nih.gov/pubmed/30532495
http://dx.doi.org/10.14202/vetworld.2018.1416-1422
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author Duskaev, G. K.
Rakhmatullin, S. G.
Kazachkova, N. M.
Sheida, Y. V.
Mikolaychik, I. N.
Morozova, L. A.
Galiev, B. H.
author_facet Duskaev, G. K.
Rakhmatullin, S. G.
Kazachkova, N. M.
Sheida, Y. V.
Mikolaychik, I. N.
Morozova, L. A.
Galiev, B. H.
author_sort Duskaev, G. K.
collection PubMed
description AIM: This study was designed to investigate the synergistic effect of the combined action of probiotic bacterial strains (Bifidobacterium adolescentis and Lactobacillus acidophilus) and Quercus cortex extract as biologically active substances in the feed on the immunity and productivity of Gallus gallus domesticus. MATERIALS AND METHODS: For the experiment, 120 7-day-old broiler chickens were selected (4 groups, n=30, 3 replicates with 10 birds in each group). The groups were as follows: The reference group - basic diet (BD); experimental Group I - BD + Q. cortex extract (Q. cortex), 2.5 ml/kg of body weight; experimental Group II - BD + probiotic preparation based on B. adolescentis, 80.0 million colony-forming units (CFU), and L. acidophilus, 1.0 million CFU (dosage in accordance with the recommendations of the manufacturer); and experimental Group III - BD + probiotic + extract of Q. cortex. The following methods of study were used: Chemiluminescence and biochemical and hematological analysis. RESULTS: The results of the experiment showed a slight decrease in the level of leukocytes in Groups II (p≤0.05) and III, and of hemoglobin in Group III (p≤0.05), compared to the reference group. The level of alanine aminotransferase and aspartate aminotransferase in Group II was higher than both the reference group (p≤0.05) and the other groups. Introduction of Q. cortex extract into the diet increased the level of triglycerides (p≤0.05) and urea in the blood serum. The combined use of probiotic preparations and the extract resulted in an increase in the level of iron in the blood serum by 78.1% (p≤0.05) in Group III. An increase in indicators of the antioxidant system (catalase increased in Group I by 27.2% (p≤0.05) and by 3.0–12.7% in other groups; superoxide dismutase increased by 3.0–13.2%) and nonspecific immunity (β-lysine increased by 8.8–16.0%) was noted. Introduction of the extract and probiotic preparation into the diet contributed to increasing the live weight of chickens at the age of 15 days by 5.9 and 7.4%, respectively (p≤0.05). In experimental Group II, this trend continued, and by the end of the period, the weight of animals exceeded that of their peers in other groups by 0.7-7.0%. Given the high preservation rate of poultry in the II and III Groups, and the low feed consumption per 1 kg of live weight gain (by 3.1–6.7%), the efficiency of growth was higher than in the reference group. CONCLUSION: Thus, the combined use of probiotic strains of bacteria and Q. cortex extract helped to increase the antioxidant activity of the organism and antimicrobial components of blood plasma compared with broiler chickens with similar growth rates but without the supplementation of this combination.
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spelling pubmed-62478752018-12-07 Effect of the combined action of Quercus cortex extract and probiotic substances on the immunity and productivity of broiler chickens Duskaev, G. K. Rakhmatullin, S. G. Kazachkova, N. M. Sheida, Y. V. Mikolaychik, I. N. Morozova, L. A. Galiev, B. H. Vet World Research Article AIM: This study was designed to investigate the synergistic effect of the combined action of probiotic bacterial strains (Bifidobacterium adolescentis and Lactobacillus acidophilus) and Quercus cortex extract as biologically active substances in the feed on the immunity and productivity of Gallus gallus domesticus. MATERIALS AND METHODS: For the experiment, 120 7-day-old broiler chickens were selected (4 groups, n=30, 3 replicates with 10 birds in each group). The groups were as follows: The reference group - basic diet (BD); experimental Group I - BD + Q. cortex extract (Q. cortex), 2.5 ml/kg of body weight; experimental Group II - BD + probiotic preparation based on B. adolescentis, 80.0 million colony-forming units (CFU), and L. acidophilus, 1.0 million CFU (dosage in accordance with the recommendations of the manufacturer); and experimental Group III - BD + probiotic + extract of Q. cortex. The following methods of study were used: Chemiluminescence and biochemical and hematological analysis. RESULTS: The results of the experiment showed a slight decrease in the level of leukocytes in Groups II (p≤0.05) and III, and of hemoglobin in Group III (p≤0.05), compared to the reference group. The level of alanine aminotransferase and aspartate aminotransferase in Group II was higher than both the reference group (p≤0.05) and the other groups. Introduction of Q. cortex extract into the diet increased the level of triglycerides (p≤0.05) and urea in the blood serum. The combined use of probiotic preparations and the extract resulted in an increase in the level of iron in the blood serum by 78.1% (p≤0.05) in Group III. An increase in indicators of the antioxidant system (catalase increased in Group I by 27.2% (p≤0.05) and by 3.0–12.7% in other groups; superoxide dismutase increased by 3.0–13.2%) and nonspecific immunity (β-lysine increased by 8.8–16.0%) was noted. Introduction of the extract and probiotic preparation into the diet contributed to increasing the live weight of chickens at the age of 15 days by 5.9 and 7.4%, respectively (p≤0.05). In experimental Group II, this trend continued, and by the end of the period, the weight of animals exceeded that of their peers in other groups by 0.7-7.0%. Given the high preservation rate of poultry in the II and III Groups, and the low feed consumption per 1 kg of live weight gain (by 3.1–6.7%), the efficiency of growth was higher than in the reference group. CONCLUSION: Thus, the combined use of probiotic strains of bacteria and Q. cortex extract helped to increase the antioxidant activity of the organism and antimicrobial components of blood plasma compared with broiler chickens with similar growth rates but without the supplementation of this combination. Veterinary World 2018-11 2018-10-13 /pmc/articles/PMC6247875/ /pubmed/30532495 http://dx.doi.org/10.14202/vetworld.2018.1416-1422 Text en Copyright: © Duskaev, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Duskaev, G. K.
Rakhmatullin, S. G.
Kazachkova, N. M.
Sheida, Y. V.
Mikolaychik, I. N.
Morozova, L. A.
Galiev, B. H.
Effect of the combined action of Quercus cortex extract and probiotic substances on the immunity and productivity of broiler chickens
title Effect of the combined action of Quercus cortex extract and probiotic substances on the immunity and productivity of broiler chickens
title_full Effect of the combined action of Quercus cortex extract and probiotic substances on the immunity and productivity of broiler chickens
title_fullStr Effect of the combined action of Quercus cortex extract and probiotic substances on the immunity and productivity of broiler chickens
title_full_unstemmed Effect of the combined action of Quercus cortex extract and probiotic substances on the immunity and productivity of broiler chickens
title_short Effect of the combined action of Quercus cortex extract and probiotic substances on the immunity and productivity of broiler chickens
title_sort effect of the combined action of quercus cortex extract and probiotic substances on the immunity and productivity of broiler chickens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247875/
https://www.ncbi.nlm.nih.gov/pubmed/30532495
http://dx.doi.org/10.14202/vetworld.2018.1416-1422
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