Cargando…
Differential roles of gangliosides in malignant properties of melanomas
Ganglioside GD3 is widely expressed in human malignant melanomas, and has been reported to be involved in the increased cell proliferation and invasion. In this study, we established GM3-, GM2-, GM1-, GD3-, or GD2-expressing melanoma cell lines by transfecting cDNAs of glyscosyltransferases, and eff...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6248923/ https://www.ncbi.nlm.nih.gov/pubmed/30462668 http://dx.doi.org/10.1371/journal.pone.0206881 |
_version_ | 1783372650866802688 |
---|---|
author | Ohmi, Yuhsuke Kambe, Mariko Ohkawa, Yuki Hamamura, Kazunori Tajima, Orie Takeuchi, Rika Furukawa, Koichi Furukawa, Keiko |
author_facet | Ohmi, Yuhsuke Kambe, Mariko Ohkawa, Yuki Hamamura, Kazunori Tajima, Orie Takeuchi, Rika Furukawa, Koichi Furukawa, Keiko |
author_sort | Ohmi, Yuhsuke |
collection | PubMed |
description | Ganglioside GD3 is widely expressed in human malignant melanomas, and has been reported to be involved in the increased cell proliferation and invasion. In this study, we established GM3-, GM2-, GM1-, GD3-, or GD2-expressing melanoma cell lines by transfecting cDNAs of glyscosyltransferases, and effects of individual gangliosides on the cell phenotypes and signals were examined. The phenotypes of established ganglioside-expressing cells were quite different, i.e. cell growth increased as following order; GD2+, GD3+ > GM1+, GM2+, GM3+ cells. Cell invasion activity increased as GD3+ ≧ GM2+ > GM1+, GM3+, GD2+ cells. Intensity of cell adhesion to collagen I (CL-I) and spreading increased as GD2+ >> GD3+, GM1+ > GM2+, GM3+ cells. In particular, cell adhesion of GD2+ cells was markedly strong. As for cell migration velocity, GD2+ cells were slower than all other cells. The immunocytostaining revealed close localization of gangliosides and F-actin in lamellipodia. Immunoblotting of phosphorylated p130Cas and paxillin by serum treatment reveled that these phosphorylations were more increased in GD3+ cells than in GD2+ or GM3+ cells, while phosphorylation of Akt underwent similarly increased phosphorylation between GD3+ and GD2+ cells compared with GM3+ cells. While GD2 and GD3 enhanced cell growth, GD3 might also contribute in cell invasion. On the other hand, GD2 might contribute in the solid fixation of melanoma cells at metastasized sites. These results suggested that individual gangliosides exert distinct roles in the different aspects of melanomas by differentially regulating cytoskeletons and signaling molecules. |
format | Online Article Text |
id | pubmed-6248923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-62489232018-12-06 Differential roles of gangliosides in malignant properties of melanomas Ohmi, Yuhsuke Kambe, Mariko Ohkawa, Yuki Hamamura, Kazunori Tajima, Orie Takeuchi, Rika Furukawa, Koichi Furukawa, Keiko PLoS One Research Article Ganglioside GD3 is widely expressed in human malignant melanomas, and has been reported to be involved in the increased cell proliferation and invasion. In this study, we established GM3-, GM2-, GM1-, GD3-, or GD2-expressing melanoma cell lines by transfecting cDNAs of glyscosyltransferases, and effects of individual gangliosides on the cell phenotypes and signals were examined. The phenotypes of established ganglioside-expressing cells were quite different, i.e. cell growth increased as following order; GD2+, GD3+ > GM1+, GM2+, GM3+ cells. Cell invasion activity increased as GD3+ ≧ GM2+ > GM1+, GM3+, GD2+ cells. Intensity of cell adhesion to collagen I (CL-I) and spreading increased as GD2+ >> GD3+, GM1+ > GM2+, GM3+ cells. In particular, cell adhesion of GD2+ cells was markedly strong. As for cell migration velocity, GD2+ cells were slower than all other cells. The immunocytostaining revealed close localization of gangliosides and F-actin in lamellipodia. Immunoblotting of phosphorylated p130Cas and paxillin by serum treatment reveled that these phosphorylations were more increased in GD3+ cells than in GD2+ or GM3+ cells, while phosphorylation of Akt underwent similarly increased phosphorylation between GD3+ and GD2+ cells compared with GM3+ cells. While GD2 and GD3 enhanced cell growth, GD3 might also contribute in cell invasion. On the other hand, GD2 might contribute in the solid fixation of melanoma cells at metastasized sites. These results suggested that individual gangliosides exert distinct roles in the different aspects of melanomas by differentially regulating cytoskeletons and signaling molecules. Public Library of Science 2018-11-21 /pmc/articles/PMC6248923/ /pubmed/30462668 http://dx.doi.org/10.1371/journal.pone.0206881 Text en © 2018 Ohmi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ohmi, Yuhsuke Kambe, Mariko Ohkawa, Yuki Hamamura, Kazunori Tajima, Orie Takeuchi, Rika Furukawa, Koichi Furukawa, Keiko Differential roles of gangliosides in malignant properties of melanomas |
title | Differential roles of gangliosides in malignant properties of melanomas |
title_full | Differential roles of gangliosides in malignant properties of melanomas |
title_fullStr | Differential roles of gangliosides in malignant properties of melanomas |
title_full_unstemmed | Differential roles of gangliosides in malignant properties of melanomas |
title_short | Differential roles of gangliosides in malignant properties of melanomas |
title_sort | differential roles of gangliosides in malignant properties of melanomas |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6248923/ https://www.ncbi.nlm.nih.gov/pubmed/30462668 http://dx.doi.org/10.1371/journal.pone.0206881 |
work_keys_str_mv | AT ohmiyuhsuke differentialrolesofgangliosidesinmalignantpropertiesofmelanomas AT kambemariko differentialrolesofgangliosidesinmalignantpropertiesofmelanomas AT ohkawayuki differentialrolesofgangliosidesinmalignantpropertiesofmelanomas AT hamamurakazunori differentialrolesofgangliosidesinmalignantpropertiesofmelanomas AT tajimaorie differentialrolesofgangliosidesinmalignantpropertiesofmelanomas AT takeuchirika differentialrolesofgangliosidesinmalignantpropertiesofmelanomas AT furukawakoichi differentialrolesofgangliosidesinmalignantpropertiesofmelanomas AT furukawakeiko differentialrolesofgangliosidesinmalignantpropertiesofmelanomas |