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Expression of urotensin II is associated with placental autophagy in patients with severe preeclampsia

The aims of this study are to explore the correlation between the expressions of urotensin II (UII) and autophagic markers (LC3 and P62) in patients with severe preeclampsia (SPE). A total of 64 pregnant subjects were recruited, including 29 healthy pregnancies and 35 preeclamptic patients (7 mild p...

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Autores principales: Pan, Ya-Jing, He, Lian, Zhou, Si-Jia, Zhang, Li-Jie, Zhang, Ai-Hua, Zhao, Yang-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249200/
https://www.ncbi.nlm.nih.gov/pubmed/29991702
http://dx.doi.org/10.1038/s41371-018-0083-9
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author Pan, Ya-Jing
He, Lian
Zhou, Si-Jia
Zhang, Li-Jie
Zhang, Ai-Hua
Zhao, Yang-Yu
author_facet Pan, Ya-Jing
He, Lian
Zhou, Si-Jia
Zhang, Li-Jie
Zhang, Ai-Hua
Zhao, Yang-Yu
author_sort Pan, Ya-Jing
collection PubMed
description The aims of this study are to explore the correlation between the expressions of urotensin II (UII) and autophagic markers (LC3 and P62) in patients with severe preeclampsia (SPE). A total of 64 pregnant subjects were recruited, including 29 healthy pregnancies and 35 preeclamptic patients (7 mild preeclamptic (MPE) patients and 28 SPE patients). UII and autophagic markers expression in placenta specimens was investigated by immunohistochemistry (IHC), RT-qPCR, and western blot. IHC analysis manifested that the expressions of UII and autophagic markers were mainly located in the placental cytotrophoblast and syncytiotrophoblast. Western blot and IHC analysis both indicated that the expression of UII was significantly correlated with autophagic marker LC3II (by western blot) or LC3 (by IHC) (r = 0.495, P = 0.010; r = 0.816, P = 0.007). Moreover, SPE group had higher expression of UII and LC3II, lower expression of P62 than that of normal controls. The expression of LC3II was positively related with systolic blood pressure (SBP) and urinary protein level (SBP (r = 0.501, P = 0.003) and urine protein quantitation (r = 0.509, P = 0.022)), whereas P62 had negative correlation with SBP. We first verify that UII has positive correlation with autophagic marker LC3 in placentas of preeclampsia patients; besides, autophagic levels are positively correlated with SBP and urine protein in patients with SPE.
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spelling pubmed-62492002018-11-26 Expression of urotensin II is associated with placental autophagy in patients with severe preeclampsia Pan, Ya-Jing He, Lian Zhou, Si-Jia Zhang, Li-Jie Zhang, Ai-Hua Zhao, Yang-Yu J Hum Hypertens Article The aims of this study are to explore the correlation between the expressions of urotensin II (UII) and autophagic markers (LC3 and P62) in patients with severe preeclampsia (SPE). A total of 64 pregnant subjects were recruited, including 29 healthy pregnancies and 35 preeclamptic patients (7 mild preeclamptic (MPE) patients and 28 SPE patients). UII and autophagic markers expression in placenta specimens was investigated by immunohistochemistry (IHC), RT-qPCR, and western blot. IHC analysis manifested that the expressions of UII and autophagic markers were mainly located in the placental cytotrophoblast and syncytiotrophoblast. Western blot and IHC analysis both indicated that the expression of UII was significantly correlated with autophagic marker LC3II (by western blot) or LC3 (by IHC) (r = 0.495, P = 0.010; r = 0.816, P = 0.007). Moreover, SPE group had higher expression of UII and LC3II, lower expression of P62 than that of normal controls. The expression of LC3II was positively related with systolic blood pressure (SBP) and urinary protein level (SBP (r = 0.501, P = 0.003) and urine protein quantitation (r = 0.509, P = 0.022)), whereas P62 had negative correlation with SBP. We first verify that UII has positive correlation with autophagic marker LC3 in placentas of preeclampsia patients; besides, autophagic levels are positively correlated with SBP and urine protein in patients with SPE. Nature Publishing Group UK 2018-07-10 2018 /pmc/articles/PMC6249200/ /pubmed/29991702 http://dx.doi.org/10.1038/s41371-018-0083-9 Text en © Macmillan Publishers Limited, part of Springer Nature 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Pan, Ya-Jing
He, Lian
Zhou, Si-Jia
Zhang, Li-Jie
Zhang, Ai-Hua
Zhao, Yang-Yu
Expression of urotensin II is associated with placental autophagy in patients with severe preeclampsia
title Expression of urotensin II is associated with placental autophagy in patients with severe preeclampsia
title_full Expression of urotensin II is associated with placental autophagy in patients with severe preeclampsia
title_fullStr Expression of urotensin II is associated with placental autophagy in patients with severe preeclampsia
title_full_unstemmed Expression of urotensin II is associated with placental autophagy in patients with severe preeclampsia
title_short Expression of urotensin II is associated with placental autophagy in patients with severe preeclampsia
title_sort expression of urotensin ii is associated with placental autophagy in patients with severe preeclampsia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249200/
https://www.ncbi.nlm.nih.gov/pubmed/29991702
http://dx.doi.org/10.1038/s41371-018-0083-9
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