A time-resolved multi-omic atlas of the developing mouse stomach

The mammalian stomach is structurally highly diverse and its organ functionality critically depends on a normal embryonic development. Although there have been several studies on the morphological changes during stomach development, a system-wide analysis of the underlying molecular changes is lacki...

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Autores principales: Li, Xianju, Zhang, Chunchao, Gong, Tongqing, Ni, Xiaotian, Li, Jin’e, Zhan, Dongdong, Liu, Mingwei, Song, Lei, Ding, Chen, Xu, Jianming, Zhen, Bei, Wang, Yi, Qin, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249217/
https://www.ncbi.nlm.nih.gov/pubmed/30464175
http://dx.doi.org/10.1038/s41467-018-07463-9
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author Li, Xianju
Zhang, Chunchao
Gong, Tongqing
Ni, Xiaotian
Li, Jin’e
Zhan, Dongdong
Liu, Mingwei
Song, Lei
Ding, Chen
Xu, Jianming
Zhen, Bei
Wang, Yi
Qin, Jun
author_facet Li, Xianju
Zhang, Chunchao
Gong, Tongqing
Ni, Xiaotian
Li, Jin’e
Zhan, Dongdong
Liu, Mingwei
Song, Lei
Ding, Chen
Xu, Jianming
Zhen, Bei
Wang, Yi
Qin, Jun
author_sort Li, Xianju
collection PubMed
description The mammalian stomach is structurally highly diverse and its organ functionality critically depends on a normal embryonic development. Although there have been several studies on the morphological changes during stomach development, a system-wide analysis of the underlying molecular changes is lacking. Here, we present a comprehensive, temporal proteome and transcriptome atlas of the mouse stomach at multiple developmental stages. Quantitative analysis of 12,108 gene products allows identifying three distinct phases based on changes in proteins and RNAs and the gain of stomach functions on a longitudinal time scale. The transcriptome indicates functionally important isoforms relevant to development and identifies several functionally unannotated novel splicing junction transcripts that we validate at the peptide level. Importantly, many proteins differentially expressed in stomach development are also significantly overexpressed in diffuse-type gastric cancer. Overall, our study provides a resource to understand stomach development and its connection to gastric cancer tumorigenesis.
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spelling pubmed-62492172018-11-26 A time-resolved multi-omic atlas of the developing mouse stomach Li, Xianju Zhang, Chunchao Gong, Tongqing Ni, Xiaotian Li, Jin’e Zhan, Dongdong Liu, Mingwei Song, Lei Ding, Chen Xu, Jianming Zhen, Bei Wang, Yi Qin, Jun Nat Commun Article The mammalian stomach is structurally highly diverse and its organ functionality critically depends on a normal embryonic development. Although there have been several studies on the morphological changes during stomach development, a system-wide analysis of the underlying molecular changes is lacking. Here, we present a comprehensive, temporal proteome and transcriptome atlas of the mouse stomach at multiple developmental stages. Quantitative analysis of 12,108 gene products allows identifying three distinct phases based on changes in proteins and RNAs and the gain of stomach functions on a longitudinal time scale. The transcriptome indicates functionally important isoforms relevant to development and identifies several functionally unannotated novel splicing junction transcripts that we validate at the peptide level. Importantly, many proteins differentially expressed in stomach development are also significantly overexpressed in diffuse-type gastric cancer. Overall, our study provides a resource to understand stomach development and its connection to gastric cancer tumorigenesis. Nature Publishing Group UK 2018-11-21 /pmc/articles/PMC6249217/ /pubmed/30464175 http://dx.doi.org/10.1038/s41467-018-07463-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Xianju
Zhang, Chunchao
Gong, Tongqing
Ni, Xiaotian
Li, Jin’e
Zhan, Dongdong
Liu, Mingwei
Song, Lei
Ding, Chen
Xu, Jianming
Zhen, Bei
Wang, Yi
Qin, Jun
A time-resolved multi-omic atlas of the developing mouse stomach
title A time-resolved multi-omic atlas of the developing mouse stomach
title_full A time-resolved multi-omic atlas of the developing mouse stomach
title_fullStr A time-resolved multi-omic atlas of the developing mouse stomach
title_full_unstemmed A time-resolved multi-omic atlas of the developing mouse stomach
title_short A time-resolved multi-omic atlas of the developing mouse stomach
title_sort time-resolved multi-omic atlas of the developing mouse stomach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249217/
https://www.ncbi.nlm.nih.gov/pubmed/30464175
http://dx.doi.org/10.1038/s41467-018-07463-9
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