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Impact of uranium uptake on isotopic fractionation and endogenous element homeostasis in human neuron-like cells

The impact of natural uranium (U) on differentiated human neuron-like cells exposed to 1, 10, 125, and 250 µM of U for seven days was assessed. In particular, the effect of the U uptake on the homeostatic modulation of several endogenous elements (Mg, P, Mn, Fe, Zn, and Cu), the U isotopic fractiona...

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Autores principales: Paredes, Eduardo, Avazeri, Emilie, Malard, Véronique, Vidaud, Claude, Reiller, Pascal E., Ortega, Richard, Nonell, Anthony, Isnard, Hélène, Chartier, Frédéric, Bresson, Carole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249223/
https://www.ncbi.nlm.nih.gov/pubmed/30464301
http://dx.doi.org/10.1038/s41598-018-35413-4
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author Paredes, Eduardo
Avazeri, Emilie
Malard, Véronique
Vidaud, Claude
Reiller, Pascal E.
Ortega, Richard
Nonell, Anthony
Isnard, Hélène
Chartier, Frédéric
Bresson, Carole
author_facet Paredes, Eduardo
Avazeri, Emilie
Malard, Véronique
Vidaud, Claude
Reiller, Pascal E.
Ortega, Richard
Nonell, Anthony
Isnard, Hélène
Chartier, Frédéric
Bresson, Carole
author_sort Paredes, Eduardo
collection PubMed
description The impact of natural uranium (U) on differentiated human neuron-like cells exposed to 1, 10, 125, and 250 µM of U for seven days was assessed. In particular, the effect of the U uptake on the homeostatic modulation of several endogenous elements (Mg, P, Mn, Fe, Zn, and Cu), the U isotopic fractionation upon its incorporation by the cells and the evolution of the intracellular Cu and Zn isotopic signatures were studied. The intracellular accumulation of U was accompanied by a preferential uptake of (235)U for cells exposed to 1 and 10 µM of U, whereas no significant isotopic fractionation was observed between the extra- and the intracellular media for higher exposure U concentrations. The U uptake was also found to modulate the homeostasis of Cu, Fe, and Mn for cells exposed to 125 and 250 µM of U, but the intracellular Cu isotopic signature was not modified. The intracellular Zn isotopic signature was not modified either. The activation of the non-specific U uptake pathway might be related to this homeostatic modulation. All together, these results show that isotopic and quantitative analyses of toxic and endogenous elements are powerful tools to help deciphering the toxicity mechanisms of heavy metals.
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spelling pubmed-62492232018-11-28 Impact of uranium uptake on isotopic fractionation and endogenous element homeostasis in human neuron-like cells Paredes, Eduardo Avazeri, Emilie Malard, Véronique Vidaud, Claude Reiller, Pascal E. Ortega, Richard Nonell, Anthony Isnard, Hélène Chartier, Frédéric Bresson, Carole Sci Rep Article The impact of natural uranium (U) on differentiated human neuron-like cells exposed to 1, 10, 125, and 250 µM of U for seven days was assessed. In particular, the effect of the U uptake on the homeostatic modulation of several endogenous elements (Mg, P, Mn, Fe, Zn, and Cu), the U isotopic fractionation upon its incorporation by the cells and the evolution of the intracellular Cu and Zn isotopic signatures were studied. The intracellular accumulation of U was accompanied by a preferential uptake of (235)U for cells exposed to 1 and 10 µM of U, whereas no significant isotopic fractionation was observed between the extra- and the intracellular media for higher exposure U concentrations. The U uptake was also found to modulate the homeostasis of Cu, Fe, and Mn for cells exposed to 125 and 250 µM of U, but the intracellular Cu isotopic signature was not modified. The intracellular Zn isotopic signature was not modified either. The activation of the non-specific U uptake pathway might be related to this homeostatic modulation. All together, these results show that isotopic and quantitative analyses of toxic and endogenous elements are powerful tools to help deciphering the toxicity mechanisms of heavy metals. Nature Publishing Group UK 2018-11-21 /pmc/articles/PMC6249223/ /pubmed/30464301 http://dx.doi.org/10.1038/s41598-018-35413-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Paredes, Eduardo
Avazeri, Emilie
Malard, Véronique
Vidaud, Claude
Reiller, Pascal E.
Ortega, Richard
Nonell, Anthony
Isnard, Hélène
Chartier, Frédéric
Bresson, Carole
Impact of uranium uptake on isotopic fractionation and endogenous element homeostasis in human neuron-like cells
title Impact of uranium uptake on isotopic fractionation and endogenous element homeostasis in human neuron-like cells
title_full Impact of uranium uptake on isotopic fractionation and endogenous element homeostasis in human neuron-like cells
title_fullStr Impact of uranium uptake on isotopic fractionation and endogenous element homeostasis in human neuron-like cells
title_full_unstemmed Impact of uranium uptake on isotopic fractionation and endogenous element homeostasis in human neuron-like cells
title_short Impact of uranium uptake on isotopic fractionation and endogenous element homeostasis in human neuron-like cells
title_sort impact of uranium uptake on isotopic fractionation and endogenous element homeostasis in human neuron-like cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249223/
https://www.ncbi.nlm.nih.gov/pubmed/30464301
http://dx.doi.org/10.1038/s41598-018-35413-4
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