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Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe

Chemical modifications to nucleobases have a great influence on various cellular processes, by making gene regulation more complex, thus indicating their profound impact on aspects of heredity, growth, and disease. Here, we provide the first genome-wide map of 5-formyluracil (5fU) in living tissues...

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Detalles Bibliográficos
Autores principales: Wang, Yafen, Liu, Chaoxing, Wu, Fan, Zhang, Xiong, Liu, Sheng, Chen, Zonggui, Zeng, Weiwu, Yang, Wei, Zhang, Xiaolian, Zhou, Yu, Weng, Xiaocheng, Wu, Zhiguo, Zhou, Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249349/
https://www.ncbi.nlm.nih.gov/pubmed/30466066
http://dx.doi.org/10.1016/j.isci.2018.10.024
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author Wang, Yafen
Liu, Chaoxing
Wu, Fan
Zhang, Xiong
Liu, Sheng
Chen, Zonggui
Zeng, Weiwu
Yang, Wei
Zhang, Xiaolian
Zhou, Yu
Weng, Xiaocheng
Wu, Zhiguo
Zhou, Xiang
author_facet Wang, Yafen
Liu, Chaoxing
Wu, Fan
Zhang, Xiong
Liu, Sheng
Chen, Zonggui
Zeng, Weiwu
Yang, Wei
Zhang, Xiaolian
Zhou, Yu
Weng, Xiaocheng
Wu, Zhiguo
Zhou, Xiang
author_sort Wang, Yafen
collection PubMed
description Chemical modifications to nucleobases have a great influence on various cellular processes, by making gene regulation more complex, thus indicating their profound impact on aspects of heredity, growth, and disease. Here, we provide the first genome-wide map of 5-formyluracil (5fU) in living tissues and evaluate the potential roles for 5fU in genomics. We show that an azido derivative of (2-benzimidazolyl)acetonitrile has high selectivity for enriching 5fU-containing genomic DNA. The results have demonstrated the feasibility of using this method to determine the genome-wide distribution of 5fU. Intriguingly, most 5fU sites were found in intergenic regions and introns. Also, distribution of 5fU in human thyroid carcinoma tissues is positively correlated with binding sites of POLR2A protein, which indicates that 5fU may distributed around POLR2A-binding sites.
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spelling pubmed-62493492018-11-30 Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe Wang, Yafen Liu, Chaoxing Wu, Fan Zhang, Xiong Liu, Sheng Chen, Zonggui Zeng, Weiwu Yang, Wei Zhang, Xiaolian Zhou, Yu Weng, Xiaocheng Wu, Zhiguo Zhou, Xiang iScience Article Chemical modifications to nucleobases have a great influence on various cellular processes, by making gene regulation more complex, thus indicating their profound impact on aspects of heredity, growth, and disease. Here, we provide the first genome-wide map of 5-formyluracil (5fU) in living tissues and evaluate the potential roles for 5fU in genomics. We show that an azido derivative of (2-benzimidazolyl)acetonitrile has high selectivity for enriching 5fU-containing genomic DNA. The results have demonstrated the feasibility of using this method to determine the genome-wide distribution of 5fU. Intriguingly, most 5fU sites were found in intergenic regions and introns. Also, distribution of 5fU in human thyroid carcinoma tissues is positively correlated with binding sites of POLR2A protein, which indicates that 5fU may distributed around POLR2A-binding sites. Elsevier 2018-11-02 /pmc/articles/PMC6249349/ /pubmed/30466066 http://dx.doi.org/10.1016/j.isci.2018.10.024 Text en © 2018 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Wang, Yafen
Liu, Chaoxing
Wu, Fan
Zhang, Xiong
Liu, Sheng
Chen, Zonggui
Zeng, Weiwu
Yang, Wei
Zhang, Xiaolian
Zhou, Yu
Weng, Xiaocheng
Wu, Zhiguo
Zhou, Xiang
Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe
title Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe
title_full Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe
title_fullStr Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe
title_full_unstemmed Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe
title_short Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe
title_sort highly selective 5-formyluracil labeling and genome-wide mapping using (2-benzimidazolyl)acetonitrile probe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249349/
https://www.ncbi.nlm.nih.gov/pubmed/30466066
http://dx.doi.org/10.1016/j.isci.2018.10.024
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