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A Proteomics Approach to Profiling the Temporal Translational Response to Stress and Growth

To quantify dynamic protein synthesis rates, we developed MITNCAT, a method combining multiplexed isobaric mass tagging with pulsed SILAC (pSILAC) and bio-orthogonal non-canonical amino acid tagging (BONCAT) to label newly synthesized proteins with azidohomoalanine (Aha), thus enabling high temporal...

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Detalles Bibliográficos
Autores principales:	Rothenberg, Daniel A., Taliaferro, J. Matthew, Huber, Sabrina M., Begley, Thomas J., Dedon, Peter C., White, Forest M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249402/ https://www.ncbi.nlm.nih.gov/pubmed/30466063 http://dx.doi.org/10.1016/j.isci.2018.11.004

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