Cargando…

Resveratrol mitigates the oxidative stress mediated by hypoxic-ischemic brain injury in neonatal rats via Nrf2/HO-1 pathway

Context: Hypoxic-ischemic encephalopathy (HIE) has a high morbidity and mortality rate. Resveratrol possesses numerous biological properties including antioxidant, anti-inflammatory and neuroprotective activities. Objective: The current experiment investigates the neuroprotective efficacy of resvera...

Descripción completa

Detalles Bibliográficos
Autores principales: Gao, Yan, Fu, Rongrong, Wang, Jue, Yang, Xue, Wen, Lulu, Feng, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249550/
https://www.ncbi.nlm.nih.gov/pubmed/30460866
http://dx.doi.org/10.1080/13880209.2018.1502326
_version_ 1783372776017494016
author Gao, Yan
Fu, Rongrong
Wang, Jue
Yang, Xue
Wen, Lulu
Feng, Juan
author_facet Gao, Yan
Fu, Rongrong
Wang, Jue
Yang, Xue
Wen, Lulu
Feng, Juan
author_sort Gao, Yan
collection PubMed
description Context: Hypoxic-ischemic encephalopathy (HIE) has a high morbidity and mortality rate. Resveratrol possesses numerous biological properties including antioxidant, anti-inflammatory and neuroprotective activities. Objective: The current experiment investigates the neuroprotective efficacy of resveratrol (RESV) against HIE by modulating Nrf2/HO-1 pathway in neonatal rats. Materials and methods: Seven-day-old pups (n = 48) were divided into four groups. Group-I rats receiving 2% DMSO saline (sham), group-II rats underwent unilateral carotid artery ligation and hypoxia (92% N(2) and 8% O(2)) for 2.5 h (hypoxia-ischemia; HI), group-III and IV rats received 20 (RESV 20 + HI) or 40 mg/kg (RESV 40 + HI; group-IV) of RESV via intraperitoneal injection (ip), respectively, for 7 days prior to HI induction. Results: Pre-treatment with RESV (20 or 40) markedly reduced (p < 0.01) the cerebral oedema (86.23–71.26 or 65.24%), infarct area (33.85–19.81 or 14.30%), lipid peroxidation products, inflammatory markers [IL-1β 186–110 or 82; IL-6 255–146 or 103; TNF-α 310–204 or 137; NF-κB 205–115 or 91) p65 subunit] and significantly restored (p < 0.01) the antioxidative status by enhancing the activities of glutathione peroxidase (GPx) 5.22–6.49 or 7.78; catalase (CAT) 51–55 or 59, superoxide dismutase (SOD) 2.5–3.05 or 3.25; through marked upregulation (p < 0.01) of heme oxygenase 1 (HO-1) 0.65–0.69 or 0.73; and nuclear factor erythroid 2 related factor 2 (Nrf2) 0.73–0.86 or 0.91. Discussion and Conclusions: RESV displays its neurotherapeutic potential via upregulating the protein expression of Nrf2 and HO-1 signalling pathway and thereby attenuates oxidative stress and inflammatory response in HI-induced neonatal rats.
format Online
Article
Text
id pubmed-6249550
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-62495502018-11-26 Resveratrol mitigates the oxidative stress mediated by hypoxic-ischemic brain injury in neonatal rats via Nrf2/HO-1 pathway Gao, Yan Fu, Rongrong Wang, Jue Yang, Xue Wen, Lulu Feng, Juan Pharm Biol Research Article Context: Hypoxic-ischemic encephalopathy (HIE) has a high morbidity and mortality rate. Resveratrol possesses numerous biological properties including antioxidant, anti-inflammatory and neuroprotective activities. Objective: The current experiment investigates the neuroprotective efficacy of resveratrol (RESV) against HIE by modulating Nrf2/HO-1 pathway in neonatal rats. Materials and methods: Seven-day-old pups (n = 48) were divided into four groups. Group-I rats receiving 2% DMSO saline (sham), group-II rats underwent unilateral carotid artery ligation and hypoxia (92% N(2) and 8% O(2)) for 2.5 h (hypoxia-ischemia; HI), group-III and IV rats received 20 (RESV 20 + HI) or 40 mg/kg (RESV 40 + HI; group-IV) of RESV via intraperitoneal injection (ip), respectively, for 7 days prior to HI induction. Results: Pre-treatment with RESV (20 or 40) markedly reduced (p < 0.01) the cerebral oedema (86.23–71.26 or 65.24%), infarct area (33.85–19.81 or 14.30%), lipid peroxidation products, inflammatory markers [IL-1β 186–110 or 82; IL-6 255–146 or 103; TNF-α 310–204 or 137; NF-κB 205–115 or 91) p65 subunit] and significantly restored (p < 0.01) the antioxidative status by enhancing the activities of glutathione peroxidase (GPx) 5.22–6.49 or 7.78; catalase (CAT) 51–55 or 59, superoxide dismutase (SOD) 2.5–3.05 or 3.25; through marked upregulation (p < 0.01) of heme oxygenase 1 (HO-1) 0.65–0.69 or 0.73; and nuclear factor erythroid 2 related factor 2 (Nrf2) 0.73–0.86 or 0.91. Discussion and Conclusions: RESV displays its neurotherapeutic potential via upregulating the protein expression of Nrf2 and HO-1 signalling pathway and thereby attenuates oxidative stress and inflammatory response in HI-induced neonatal rats. Taylor & Francis 2018-11-21 /pmc/articles/PMC6249550/ /pubmed/30460866 http://dx.doi.org/10.1080/13880209.2018.1502326 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gao, Yan
Fu, Rongrong
Wang, Jue
Yang, Xue
Wen, Lulu
Feng, Juan
Resveratrol mitigates the oxidative stress mediated by hypoxic-ischemic brain injury in neonatal rats via Nrf2/HO-1 pathway
title Resveratrol mitigates the oxidative stress mediated by hypoxic-ischemic brain injury in neonatal rats via Nrf2/HO-1 pathway
title_full Resveratrol mitigates the oxidative stress mediated by hypoxic-ischemic brain injury in neonatal rats via Nrf2/HO-1 pathway
title_fullStr Resveratrol mitigates the oxidative stress mediated by hypoxic-ischemic brain injury in neonatal rats via Nrf2/HO-1 pathway
title_full_unstemmed Resveratrol mitigates the oxidative stress mediated by hypoxic-ischemic brain injury in neonatal rats via Nrf2/HO-1 pathway
title_short Resveratrol mitigates the oxidative stress mediated by hypoxic-ischemic brain injury in neonatal rats via Nrf2/HO-1 pathway
title_sort resveratrol mitigates the oxidative stress mediated by hypoxic-ischemic brain injury in neonatal rats via nrf2/ho-1 pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249550/
https://www.ncbi.nlm.nih.gov/pubmed/30460866
http://dx.doi.org/10.1080/13880209.2018.1502326
work_keys_str_mv AT gaoyan resveratrolmitigatestheoxidativestressmediatedbyhypoxicischemicbraininjuryinneonatalratsvianrf2ho1pathway
AT furongrong resveratrolmitigatestheoxidativestressmediatedbyhypoxicischemicbraininjuryinneonatalratsvianrf2ho1pathway
AT wangjue resveratrolmitigatestheoxidativestressmediatedbyhypoxicischemicbraininjuryinneonatalratsvianrf2ho1pathway
AT yangxue resveratrolmitigatestheoxidativestressmediatedbyhypoxicischemicbraininjuryinneonatalratsvianrf2ho1pathway
AT wenlulu resveratrolmitigatestheoxidativestressmediatedbyhypoxicischemicbraininjuryinneonatalratsvianrf2ho1pathway
AT fengjuan resveratrolmitigatestheoxidativestressmediatedbyhypoxicischemicbraininjuryinneonatalratsvianrf2ho1pathway