Tracking yeast pheromone receptor Ste2 endocytosis using fluorogen-activating protein tagging

To observe internalization of the yeast pheromone receptor Ste2 by fluorescence microscopy in live cells in real time, we visualized only those molecules present at the cell surface at the time of agonist engagement (rather than the total cellular pool) by tagging this receptor at its N-terminus wit...

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Detalles Bibliográficos
Autores principales: Emmerstorfer-Augustin, Anita, Augustin, Christoph M., Shams, Shadi, Thorner, Jeremy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249837/
https://www.ncbi.nlm.nih.gov/pubmed/30207829
http://dx.doi.org/10.1091/mbc.E18-07-0424

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249837/
https://www.ncbi.nlm.nih.gov/pubmed/30207829
http://dx.doi.org/10.1091/mbc.E18-07-0424

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