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Whole genome mRNA expression profiling revealed multiple deregulated pathways in stromal vascular fraction from erectile dysfunction patients

Background: Stem-cell-based therapies have recently been explored in the field of erectile dysfunction (ED). However, the cellular and molecular phenotype of adipose derived stem cells (ADSCs) stromal vascular fraction (SVF) from ED patients remains largely unknown. Herein we compared the global gen...

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Autores principales: Vishnubalaji, Radhakrishnan, Manikandan, Muthurangan, Aldahmash, Abdullah, AlJarbou, Abdullah, Habous, Mohamad, Alhajeri, Dulaim, Almannie, Raed, Alfayez, Musaad, Alajez, Nehad M., Binsaleh, Saleh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6250806/
https://www.ncbi.nlm.nih.gov/pubmed/30333254
http://dx.doi.org/10.1042/BSR20181015
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author Vishnubalaji, Radhakrishnan
Manikandan, Muthurangan
Aldahmash, Abdullah
AlJarbou, Abdullah
Habous, Mohamad
Alhajeri, Dulaim
Almannie, Raed
Alfayez, Musaad
Alajez, Nehad M.
Binsaleh, Saleh
author_facet Vishnubalaji, Radhakrishnan
Manikandan, Muthurangan
Aldahmash, Abdullah
AlJarbou, Abdullah
Habous, Mohamad
Alhajeri, Dulaim
Almannie, Raed
Alfayez, Musaad
Alajez, Nehad M.
Binsaleh, Saleh
author_sort Vishnubalaji, Radhakrishnan
collection PubMed
description Background: Stem-cell-based therapies have recently been explored in the field of erectile dysfunction (ED). However, the cellular and molecular phenotype of adipose derived stem cells (ADSCs) stromal vascular fraction (SVF) from ED patients remains largely unknown. Herein we compared the global gene expression profile in the SVF from ED patients and healthy individuals and identified altered signaling pathways between the two groups. Methods: Samples (2–5 g) of abdominal adipose tissue from ED patients (n = 6) and healthy individual controls (n = 3) undergoing elective cosmetic liposuction were collected. Immediately after removal, SVF was separated using Collagenase type I and type IV protocol. RNA was isolated and microarray experiments were conducted using the Agilent platform. Data were normalized and pathway analyses were performed using GeneSpring software. Results: Our data revealed multiple differentially expressed genes between the ED and control group. Hierarchical clustering based on differentially expressed mRNAs revealed clear separation of the two groups. The distribution of the top enriched pathways for the up-regulated genes indicated enrichment in inflammatory response and T-cell receptor signaling, while pathway analysis performed on the down-regulated genes revealed enrichment in mitogen-activated protein kinase, TGF-β, senescence, FAK, adipogenesis, androgen receptor, and EGF–EGFR signaling pathways in SVF from ED patient. Conclusion: Our data revealed the existence of multiple altered signaling pathways in the SVF from ED patients, which could potentially play a role in the etiology of this disease. Therefore, therapeutic strategies targeting these pathways might provide novel therapeutic opportunity for ED patients.
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spelling pubmed-62508062018-12-04 Whole genome mRNA expression profiling revealed multiple deregulated pathways in stromal vascular fraction from erectile dysfunction patients Vishnubalaji, Radhakrishnan Manikandan, Muthurangan Aldahmash, Abdullah AlJarbou, Abdullah Habous, Mohamad Alhajeri, Dulaim Almannie, Raed Alfayez, Musaad Alajez, Nehad M. Binsaleh, Saleh Biosci Rep Research Articles Background: Stem-cell-based therapies have recently been explored in the field of erectile dysfunction (ED). However, the cellular and molecular phenotype of adipose derived stem cells (ADSCs) stromal vascular fraction (SVF) from ED patients remains largely unknown. Herein we compared the global gene expression profile in the SVF from ED patients and healthy individuals and identified altered signaling pathways between the two groups. Methods: Samples (2–5 g) of abdominal adipose tissue from ED patients (n = 6) and healthy individual controls (n = 3) undergoing elective cosmetic liposuction were collected. Immediately after removal, SVF was separated using Collagenase type I and type IV protocol. RNA was isolated and microarray experiments were conducted using the Agilent platform. Data were normalized and pathway analyses were performed using GeneSpring software. Results: Our data revealed multiple differentially expressed genes between the ED and control group. Hierarchical clustering based on differentially expressed mRNAs revealed clear separation of the two groups. The distribution of the top enriched pathways for the up-regulated genes indicated enrichment in inflammatory response and T-cell receptor signaling, while pathway analysis performed on the down-regulated genes revealed enrichment in mitogen-activated protein kinase, TGF-β, senescence, FAK, adipogenesis, androgen receptor, and EGF–EGFR signaling pathways in SVF from ED patient. Conclusion: Our data revealed the existence of multiple altered signaling pathways in the SVF from ED patients, which could potentially play a role in the etiology of this disease. Therefore, therapeutic strategies targeting these pathways might provide novel therapeutic opportunity for ED patients. Portland Press Ltd. 2018-11-23 /pmc/articles/PMC6250806/ /pubmed/30333254 http://dx.doi.org/10.1042/BSR20181015 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Vishnubalaji, Radhakrishnan
Manikandan, Muthurangan
Aldahmash, Abdullah
AlJarbou, Abdullah
Habous, Mohamad
Alhajeri, Dulaim
Almannie, Raed
Alfayez, Musaad
Alajez, Nehad M.
Binsaleh, Saleh
Whole genome mRNA expression profiling revealed multiple deregulated pathways in stromal vascular fraction from erectile dysfunction patients
title Whole genome mRNA expression profiling revealed multiple deregulated pathways in stromal vascular fraction from erectile dysfunction patients
title_full Whole genome mRNA expression profiling revealed multiple deregulated pathways in stromal vascular fraction from erectile dysfunction patients
title_fullStr Whole genome mRNA expression profiling revealed multiple deregulated pathways in stromal vascular fraction from erectile dysfunction patients
title_full_unstemmed Whole genome mRNA expression profiling revealed multiple deregulated pathways in stromal vascular fraction from erectile dysfunction patients
title_short Whole genome mRNA expression profiling revealed multiple deregulated pathways in stromal vascular fraction from erectile dysfunction patients
title_sort whole genome mrna expression profiling revealed multiple deregulated pathways in stromal vascular fraction from erectile dysfunction patients
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6250806/
https://www.ncbi.nlm.nih.gov/pubmed/30333254
http://dx.doi.org/10.1042/BSR20181015
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