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Pressure-dependent NOS activation contributes to endothelial hyperpermeability in a model of acute heart failure
Aims: Acute increases in left ventricular end diastolic pressure (LVEDP) can induce pulmonary edema (PE). The mechanism(s) for this rapid onset edema may involve more than just increased fluid filtration. Lung endothelial cell permeability is regulated by pressure-dependent activation of nitric oxid...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6250809/ https://www.ncbi.nlm.nih.gov/pubmed/30355657 http://dx.doi.org/10.1042/BSR20181239 |
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author | Chignalia, Andreia Z. Isbatan, Ayman Patel, Milan Ripper, Richard Sharlin, Jordan Shosfy, Joelle Borlaug, Barry A. Dull, Randal O. |
author_facet | Chignalia, Andreia Z. Isbatan, Ayman Patel, Milan Ripper, Richard Sharlin, Jordan Shosfy, Joelle Borlaug, Barry A. Dull, Randal O. |
author_sort | Chignalia, Andreia Z. |
collection | PubMed |
description | Aims: Acute increases in left ventricular end diastolic pressure (LVEDP) can induce pulmonary edema (PE). The mechanism(s) for this rapid onset edema may involve more than just increased fluid filtration. Lung endothelial cell permeability is regulated by pressure-dependent activation of nitric oxide synthase (NOS). Herein, we demonstrate that pressure-dependent NOS activation contributes to vascular failure and PE in a model of acute heart failure (AHF) caused by hypertension. Methods and results: Male Sprague–Dawley rats were anesthetized and mechanically ventilated. Acute hypertension was induced by norepinephrine (NE) infusion and resulted in an increase in LVEDP and pulmonary artery pressure (P(pa)) that were associated with a rapid fall in P(a)O(2), and increases in lung wet/dry ratio and injury scores. Heart failure (HF) lungs showed increased nitrotyrosine content and ROS levels. L-NAME pretreatment mitigated the development of PE and reduced lung ROS concentrations to sham levels. Apocynin (Apo) pretreatment inhibited PE. Addition of tetrahydrobiopterin (BH4) to AHF rats lung lysates and pretreatment of AHF rats with folic acid (FA) prevented ROS production indicating endothelial NOS (eNOS) uncoupling. Conclusion: Pressure-dependent NOS activation leads to acute endothelial hyperpermeability and rapid PE by an increase in NO and ROS in a model of AHF. Acute increases in pulmonary vascular pressure, without NOS activation, was insufficient to cause significant PE. These results suggest a clinically relevant role of endothelial mechanotransduction in the pathogenesis of AHF and further highlights the concept of active barrier failure in AHF. Therapies targetting the prevention or reversal of endothelial hyperpermeability may be a novel therapeutic strategy in AHF. |
format | Online Article Text |
id | pubmed-6250809 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62508092018-12-04 Pressure-dependent NOS activation contributes to endothelial hyperpermeability in a model of acute heart failure Chignalia, Andreia Z. Isbatan, Ayman Patel, Milan Ripper, Richard Sharlin, Jordan Shosfy, Joelle Borlaug, Barry A. Dull, Randal O. Biosci Rep Research Articles Aims: Acute increases in left ventricular end diastolic pressure (LVEDP) can induce pulmonary edema (PE). The mechanism(s) for this rapid onset edema may involve more than just increased fluid filtration. Lung endothelial cell permeability is regulated by pressure-dependent activation of nitric oxide synthase (NOS). Herein, we demonstrate that pressure-dependent NOS activation contributes to vascular failure and PE in a model of acute heart failure (AHF) caused by hypertension. Methods and results: Male Sprague–Dawley rats were anesthetized and mechanically ventilated. Acute hypertension was induced by norepinephrine (NE) infusion and resulted in an increase in LVEDP and pulmonary artery pressure (P(pa)) that were associated with a rapid fall in P(a)O(2), and increases in lung wet/dry ratio and injury scores. Heart failure (HF) lungs showed increased nitrotyrosine content and ROS levels. L-NAME pretreatment mitigated the development of PE and reduced lung ROS concentrations to sham levels. Apocynin (Apo) pretreatment inhibited PE. Addition of tetrahydrobiopterin (BH4) to AHF rats lung lysates and pretreatment of AHF rats with folic acid (FA) prevented ROS production indicating endothelial NOS (eNOS) uncoupling. Conclusion: Pressure-dependent NOS activation leads to acute endothelial hyperpermeability and rapid PE by an increase in NO and ROS in a model of AHF. Acute increases in pulmonary vascular pressure, without NOS activation, was insufficient to cause significant PE. These results suggest a clinically relevant role of endothelial mechanotransduction in the pathogenesis of AHF and further highlights the concept of active barrier failure in AHF. Therapies targetting the prevention or reversal of endothelial hyperpermeability may be a novel therapeutic strategy in AHF. Portland Press Ltd. 2018-11-23 /pmc/articles/PMC6250809/ /pubmed/30355657 http://dx.doi.org/10.1042/BSR20181239 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Chignalia, Andreia Z. Isbatan, Ayman Patel, Milan Ripper, Richard Sharlin, Jordan Shosfy, Joelle Borlaug, Barry A. Dull, Randal O. Pressure-dependent NOS activation contributes to endothelial hyperpermeability in a model of acute heart failure |
title | Pressure-dependent NOS activation contributes to endothelial hyperpermeability in a model of acute heart failure |
title_full | Pressure-dependent NOS activation contributes to endothelial hyperpermeability in a model of acute heart failure |
title_fullStr | Pressure-dependent NOS activation contributes to endothelial hyperpermeability in a model of acute heart failure |
title_full_unstemmed | Pressure-dependent NOS activation contributes to endothelial hyperpermeability in a model of acute heart failure |
title_short | Pressure-dependent NOS activation contributes to endothelial hyperpermeability in a model of acute heart failure |
title_sort | pressure-dependent nos activation contributes to endothelial hyperpermeability in a model of acute heart failure |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6250809/ https://www.ncbi.nlm.nih.gov/pubmed/30355657 http://dx.doi.org/10.1042/BSR20181239 |
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