A modified high-throughput analysis of PLFAs in soil

Microbial community profiling via phospholipid fatty-acid (PLFA) analysis is an insightful technique which elucidates the phenotypic structure of microbial assemblages within soil. Previous iterations of PLFA analysis have used large quantities of chemicals and can take extended periods of time to p...

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Detalles Bibliográficos
Autores principales: Ellis, Stephanie, Ritz, Karl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Environmental Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251023/
https://www.ncbi.nlm.nih.gov/pubmed/30505703
http://dx.doi.org/10.1016/j.mex.2018.10.022
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author Ellis, Stephanie
Ritz, Karl
author_facet Ellis, Stephanie
Ritz, Karl
author_sort Ellis, Stephanie
collection PubMed
description Microbial community profiling via phospholipid fatty-acid (PLFA) analysis is an insightful technique which elucidates the phenotypic structure of microbial assemblages within soil. Previous iterations of PLFA analysis have used large quantities of chemicals and can take extended periods of time to perform. Another barrier to the implementation of this method is the cost and availability of specialised machinery. We report on a high-throughput method which reduces both the time to extract PLFAs from soil and reduces the quantity of chemicals required.
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spelling pubmed-62510232018-11-30 A modified high-throughput analysis of PLFAs in soil Ellis, Stephanie Ritz, Karl MethodsX Environmental Science Microbial community profiling via phospholipid fatty-acid (PLFA) analysis is an insightful technique which elucidates the phenotypic structure of microbial assemblages within soil. Previous iterations of PLFA analysis have used large quantities of chemicals and can take extended periods of time to perform. Another barrier to the implementation of this method is the cost and availability of specialised machinery. We report on a high-throughput method which reduces both the time to extract PLFAs from soil and reduces the quantity of chemicals required. Elsevier 2018-10-31 /pmc/articles/PMC6251023/ /pubmed/30505703 http://dx.doi.org/10.1016/j.mex.2018.10.022 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Environmental Science
Ellis, Stephanie
Ritz, Karl
A modified high-throughput analysis of PLFAs in soil
title A modified high-throughput analysis of PLFAs in soil
title_full A modified high-throughput analysis of PLFAs in soil
title_fullStr A modified high-throughput analysis of PLFAs in soil
title_full_unstemmed A modified high-throughput analysis of PLFAs in soil
title_short A modified high-throughput analysis of PLFAs in soil
title_sort modified high-throughput analysis of plfas in soil
topic Environmental Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251023/
https://www.ncbi.nlm.nih.gov/pubmed/30505703
http://dx.doi.org/10.1016/j.mex.2018.10.022
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