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Gene expression in reproductive organs of tsetse females – initial data in an approach to reduce fecundity

BACKGROUND: Tsetse flies are vectors of African trypanosomes, and their vectorial capacity results in a major public health emergency and vast economic losses in sub-Saharan Africa. Given the limited ability of trypanosome prevention and eradication, tsetse vectors remain major targets of control ef...

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Autores principales: Procházka, Emanuel, Michalková, Veronika, Daubnerová, Ivana, Roller, Ladislav, Klepsatel, Peter, Žitňan, Dušan, Tsiamis, George, Takáč, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251150/
https://www.ncbi.nlm.nih.gov/pubmed/30470199
http://dx.doi.org/10.1186/s12866-018-1294-5
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author Procházka, Emanuel
Michalková, Veronika
Daubnerová, Ivana
Roller, Ladislav
Klepsatel, Peter
Žitňan, Dušan
Tsiamis, George
Takáč, Peter
author_facet Procházka, Emanuel
Michalková, Veronika
Daubnerová, Ivana
Roller, Ladislav
Klepsatel, Peter
Žitňan, Dušan
Tsiamis, George
Takáč, Peter
author_sort Procházka, Emanuel
collection PubMed
description BACKGROUND: Tsetse flies are vectors of African trypanosomes, and their vectorial capacity results in a major public health emergency and vast economic losses in sub-Saharan Africa. Given the limited ability of trypanosome prevention and eradication, tsetse vectors remain major targets of control efforts. Larvae of all three instars are developed in mothers’ uteri, nourished through milk, and ‘larviposited’ shortly before pupation. The past few years have witnessed the emergence of approaches based on knockdown of genes involved in milk production, resulting in a significant reduction of fecundity. RESULTS: In order to identify further genes applicable in the control of tsetse flies, we determined the expression of protein-coding genes in ovaries and uteri from both virgin and heavily pregnant Glossina morsitans morsitans females. Comparison of expression profiles allowed us to identify candidate genes with increased expression in pregnant individuals. Lists with the highest increases include genes involved in oocyte and embryonic development, or nourishment. Maximum ovarian fold change does not exceed 700, while the highest uterine fold change reaches to more than 4000. Relatively high fold changes of two neuropeptide receptors (for corazonin and myosuppressin) propose the corresponding genes alternative targets. CONCLUSIONS: Given the higher fold changes in the uterus, targeting gene expression in this tissue may result in a more evident reduction of fecundity. However, ovaries should not be neglected, as manifested by several genes with top fold changes involved in early developmental stages. Apart from focusing on the highest fold changes, neuropeptide receptors with moderate increases in expression should be also verified as targets, given their roles in mediating the tissue control. However, this data needs to be considered initial, and the potential of these genes in affecting female fecundity needs to be verified experimentally. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-018-1294-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-62511502018-11-26 Gene expression in reproductive organs of tsetse females – initial data in an approach to reduce fecundity Procházka, Emanuel Michalková, Veronika Daubnerová, Ivana Roller, Ladislav Klepsatel, Peter Žitňan, Dušan Tsiamis, George Takáč, Peter BMC Microbiol Research BACKGROUND: Tsetse flies are vectors of African trypanosomes, and their vectorial capacity results in a major public health emergency and vast economic losses in sub-Saharan Africa. Given the limited ability of trypanosome prevention and eradication, tsetse vectors remain major targets of control efforts. Larvae of all three instars are developed in mothers’ uteri, nourished through milk, and ‘larviposited’ shortly before pupation. The past few years have witnessed the emergence of approaches based on knockdown of genes involved in milk production, resulting in a significant reduction of fecundity. RESULTS: In order to identify further genes applicable in the control of tsetse flies, we determined the expression of protein-coding genes in ovaries and uteri from both virgin and heavily pregnant Glossina morsitans morsitans females. Comparison of expression profiles allowed us to identify candidate genes with increased expression in pregnant individuals. Lists with the highest increases include genes involved in oocyte and embryonic development, or nourishment. Maximum ovarian fold change does not exceed 700, while the highest uterine fold change reaches to more than 4000. Relatively high fold changes of two neuropeptide receptors (for corazonin and myosuppressin) propose the corresponding genes alternative targets. CONCLUSIONS: Given the higher fold changes in the uterus, targeting gene expression in this tissue may result in a more evident reduction of fecundity. However, ovaries should not be neglected, as manifested by several genes with top fold changes involved in early developmental stages. Apart from focusing on the highest fold changes, neuropeptide receptors with moderate increases in expression should be also verified as targets, given their roles in mediating the tissue control. However, this data needs to be considered initial, and the potential of these genes in affecting female fecundity needs to be verified experimentally. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-018-1294-5) contains supplementary material, which is available to authorized users. BioMed Central 2018-11-23 /pmc/articles/PMC6251150/ /pubmed/30470199 http://dx.doi.org/10.1186/s12866-018-1294-5 Text en © International Atomic Energy Agency; licensee BioMed Central Ltd. 2018 This is an open access article distributed under the terms of the Creative Commons Attribution IGO License (https://creativecommons.org/licenses/by/3.0/igo/) which permits unrestricted use, distribution, and reproduction in any medium, provided appropriate credit to the original author(s) and the source is given.
spellingShingle Research
Procházka, Emanuel
Michalková, Veronika
Daubnerová, Ivana
Roller, Ladislav
Klepsatel, Peter
Žitňan, Dušan
Tsiamis, George
Takáč, Peter
Gene expression in reproductive organs of tsetse females – initial data in an approach to reduce fecundity
title Gene expression in reproductive organs of tsetse females – initial data in an approach to reduce fecundity
title_full Gene expression in reproductive organs of tsetse females – initial data in an approach to reduce fecundity
title_fullStr Gene expression in reproductive organs of tsetse females – initial data in an approach to reduce fecundity
title_full_unstemmed Gene expression in reproductive organs of tsetse females – initial data in an approach to reduce fecundity
title_short Gene expression in reproductive organs of tsetse females – initial data in an approach to reduce fecundity
title_sort gene expression in reproductive organs of tsetse females – initial data in an approach to reduce fecundity
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251150/
https://www.ncbi.nlm.nih.gov/pubmed/30470199
http://dx.doi.org/10.1186/s12866-018-1294-5
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