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Witches’ broom resistant genotype CCN51 shows greater diversity of symbiont bacteria in its phylloplane than susceptible genotype catongo
BACKGROUND: Theobroma cacao L. (cacao) is a perennial tropical tree, endemic to rainforests of the Amazon Basin. Large populations of bacteria live on leaf surfaces and these phylloplane microorganisms can have important effects on plant health. In recent years, the advent of high-throughput sequenc...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251189/ https://www.ncbi.nlm.nih.gov/pubmed/30470193 http://dx.doi.org/10.1186/s12866-018-1339-9 |
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author | Santana, Juliano Oliveira Gramacho, Karina Peres de Souza Eduvirgens Ferreira, Katiúcia Tícila Rezende, Rachel Passos Mangabeira, Pedro Antônio Oliveira Dias, Ricardo Pedro Moreira Couto, Francisco M. Pirovani, Carlos Priminho |
author_facet | Santana, Juliano Oliveira Gramacho, Karina Peres de Souza Eduvirgens Ferreira, Katiúcia Tícila Rezende, Rachel Passos Mangabeira, Pedro Antônio Oliveira Dias, Ricardo Pedro Moreira Couto, Francisco M. Pirovani, Carlos Priminho |
author_sort | Santana, Juliano Oliveira |
collection | PubMed |
description | BACKGROUND: Theobroma cacao L. (cacao) is a perennial tropical tree, endemic to rainforests of the Amazon Basin. Large populations of bacteria live on leaf surfaces and these phylloplane microorganisms can have important effects on plant health. In recent years, the advent of high-throughput sequencing techniques has greatly facilitated studies of the phylloplane microbiome. In this study, we characterized the bacterial microbiome of the phylloplane of the catongo genotype (susceptible to witch’s broom) and CCN51 (resistant). Bacterial microbiome was determined by sequencing the V3-V4 region of the bacterial 16S rRNA gene. RESULTS: After the pre-processing, a total of 1.7 million reads were considered. In total, 106 genera of bacteria were characterized. Proteobacteria was the predominant phylum in both genotypes. The exclusive genera of Catongo showed activity in the protection against UV radiation and in the transport of substrates. CCN51 presented genus that act in the biological control and inhibition in several taxonomic groups. Genotype CCN51 presented greater diversity of microorganisms in comparison to the Catongo genotype and the total community was different between both. Scanning electron microscopy analysis of leaves revealed that on the phylloplane, many bacterial occur in large aggregates in several regions of the surface and isolated nearby to the stomata. CONCLUSIONS: We describe for the first time the phylloplane bacterial communities of T. cacao. The Genotype CCN51, resistant to the witch’s broom, has a greater diversity of bacterial microbioma in comparison to Catongo and a greater amount of exclusive microorganisms in the phylloplane with antagonistic action against phytopathogens. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-018-1339-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6251189 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62511892018-11-29 Witches’ broom resistant genotype CCN51 shows greater diversity of symbiont bacteria in its phylloplane than susceptible genotype catongo Santana, Juliano Oliveira Gramacho, Karina Peres de Souza Eduvirgens Ferreira, Katiúcia Tícila Rezende, Rachel Passos Mangabeira, Pedro Antônio Oliveira Dias, Ricardo Pedro Moreira Couto, Francisco M. Pirovani, Carlos Priminho BMC Microbiol Research Article BACKGROUND: Theobroma cacao L. (cacao) is a perennial tropical tree, endemic to rainforests of the Amazon Basin. Large populations of bacteria live on leaf surfaces and these phylloplane microorganisms can have important effects on plant health. In recent years, the advent of high-throughput sequencing techniques has greatly facilitated studies of the phylloplane microbiome. In this study, we characterized the bacterial microbiome of the phylloplane of the catongo genotype (susceptible to witch’s broom) and CCN51 (resistant). Bacterial microbiome was determined by sequencing the V3-V4 region of the bacterial 16S rRNA gene. RESULTS: After the pre-processing, a total of 1.7 million reads were considered. In total, 106 genera of bacteria were characterized. Proteobacteria was the predominant phylum in both genotypes. The exclusive genera of Catongo showed activity in the protection against UV radiation and in the transport of substrates. CCN51 presented genus that act in the biological control and inhibition in several taxonomic groups. Genotype CCN51 presented greater diversity of microorganisms in comparison to the Catongo genotype and the total community was different between both. Scanning electron microscopy analysis of leaves revealed that on the phylloplane, many bacterial occur in large aggregates in several regions of the surface and isolated nearby to the stomata. CONCLUSIONS: We describe for the first time the phylloplane bacterial communities of T. cacao. The Genotype CCN51, resistant to the witch’s broom, has a greater diversity of bacterial microbioma in comparison to Catongo and a greater amount of exclusive microorganisms in the phylloplane with antagonistic action against phytopathogens. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-018-1339-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-11-23 /pmc/articles/PMC6251189/ /pubmed/30470193 http://dx.doi.org/10.1186/s12866-018-1339-9 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Santana, Juliano Oliveira Gramacho, Karina Peres de Souza Eduvirgens Ferreira, Katiúcia Tícila Rezende, Rachel Passos Mangabeira, Pedro Antônio Oliveira Dias, Ricardo Pedro Moreira Couto, Francisco M. Pirovani, Carlos Priminho Witches’ broom resistant genotype CCN51 shows greater diversity of symbiont bacteria in its phylloplane than susceptible genotype catongo |
title | Witches’ broom resistant genotype CCN51 shows greater diversity of symbiont bacteria in its phylloplane than susceptible genotype catongo |
title_full | Witches’ broom resistant genotype CCN51 shows greater diversity of symbiont bacteria in its phylloplane than susceptible genotype catongo |
title_fullStr | Witches’ broom resistant genotype CCN51 shows greater diversity of symbiont bacteria in its phylloplane than susceptible genotype catongo |
title_full_unstemmed | Witches’ broom resistant genotype CCN51 shows greater diversity of symbiont bacteria in its phylloplane than susceptible genotype catongo |
title_short | Witches’ broom resistant genotype CCN51 shows greater diversity of symbiont bacteria in its phylloplane than susceptible genotype catongo |
title_sort | witches’ broom resistant genotype ccn51 shows greater diversity of symbiont bacteria in its phylloplane than susceptible genotype catongo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251189/ https://www.ncbi.nlm.nih.gov/pubmed/30470193 http://dx.doi.org/10.1186/s12866-018-1339-9 |
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