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The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype
OBJECTIVE(S): Crocetin, one of the main substances of saffron extract, has anti-cancer effects. Drug resistance proteins (e.g. MRP1 and MRP2) are important reasons for the failure of cancer therapy. We intended to investigate the efficacy of crocetin on MRP(1) and MRP(2) activity in human ovarian ci...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251388/ https://www.ncbi.nlm.nih.gov/pubmed/30483395 http://dx.doi.org/10.22038/IJBMS.2018.29474.7118 |
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author | Neyshaburinezhad, Navid Hashemi, Maryam Ramezani, Mohammad Arabzadeh, Sepideh Behravan, Javad Kalalinia, Fatemeh |
author_facet | Neyshaburinezhad, Navid Hashemi, Maryam Ramezani, Mohammad Arabzadeh, Sepideh Behravan, Javad Kalalinia, Fatemeh |
author_sort | Neyshaburinezhad, Navid |
collection | PubMed |
description | OBJECTIVE(S): Crocetin, one of the main substances of saffron extract, has anti-cancer effects. Drug resistance proteins (e.g. MRP1 and MRP2) are important reasons for the failure of cancer therapy. We intended to investigate the efficacy of crocetin on MRP(1) and MRP(2) activity in human ovarian cisplatin-resistant carcinoma cell line (A2780-RCIS). MATERIALS AND METHODS: The cytotoxic effect of crocetin was evaluated by the MTT assay. The efficacy of crocetin on MRP(1) and MRP(2) expression at mRNA level was studied by real-time RT-PCR. The effect of crocetin on the activity of MRP transporters was determined by drug efflux assay. RESULTS: Crocetin decreased cell proliferation in the A2780 (IC(50): 183±7 µM) and A2780-RCIS (IC(50): 316±9 µM). Crocetin decreased the expression level of MRP1 (22±2 %) and MRP2 (48±8 %) in A2780-RCIS and inhibited MRP pumps function directly in A2780 (44±1 %) and A2780-RCIS (88±10 %) and indirectly in A2780 (32±2 %) and A2780-RCIS (48±15 %) respectively. CONCLUSION: Our findings showed that crocetin could quench drug resistance through modulation of MRP transporters in the drug resistant human ovarian cancer cells. |
format | Online Article Text |
id | pubmed-6251388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-62513882018-11-27 The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype Neyshaburinezhad, Navid Hashemi, Maryam Ramezani, Mohammad Arabzadeh, Sepideh Behravan, Javad Kalalinia, Fatemeh Iran J Basic Med Sci Short Communication OBJECTIVE(S): Crocetin, one of the main substances of saffron extract, has anti-cancer effects. Drug resistance proteins (e.g. MRP1 and MRP2) are important reasons for the failure of cancer therapy. We intended to investigate the efficacy of crocetin on MRP(1) and MRP(2) activity in human ovarian cisplatin-resistant carcinoma cell line (A2780-RCIS). MATERIALS AND METHODS: The cytotoxic effect of crocetin was evaluated by the MTT assay. The efficacy of crocetin on MRP(1) and MRP(2) expression at mRNA level was studied by real-time RT-PCR. The effect of crocetin on the activity of MRP transporters was determined by drug efflux assay. RESULTS: Crocetin decreased cell proliferation in the A2780 (IC(50): 183±7 µM) and A2780-RCIS (IC(50): 316±9 µM). Crocetin decreased the expression level of MRP1 (22±2 %) and MRP2 (48±8 %) in A2780-RCIS and inhibited MRP pumps function directly in A2780 (44±1 %) and A2780-RCIS (88±10 %) and indirectly in A2780 (32±2 %) and A2780-RCIS (48±15 %) respectively. CONCLUSION: Our findings showed that crocetin could quench drug resistance through modulation of MRP transporters in the drug resistant human ovarian cancer cells. Mashhad University of Medical Sciences 2018-11 /pmc/articles/PMC6251388/ /pubmed/30483395 http://dx.doi.org/10.22038/IJBMS.2018.29474.7118 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Neyshaburinezhad, Navid Hashemi, Maryam Ramezani, Mohammad Arabzadeh, Sepideh Behravan, Javad Kalalinia, Fatemeh The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype |
title | The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype |
title_full | The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype |
title_fullStr | The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype |
title_full_unstemmed | The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype |
title_short | The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype |
title_sort | effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251388/ https://www.ncbi.nlm.nih.gov/pubmed/30483395 http://dx.doi.org/10.22038/IJBMS.2018.29474.7118 |
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