Cargando…

The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype

OBJECTIVE(S): Crocetin, one of the main substances of saffron extract, has anti-cancer effects. Drug resistance proteins (e.g. MRP1 and MRP2) are important reasons for the failure of cancer therapy. We intended to investigate the efficacy of crocetin on MRP(1) and MRP(2) activity in human ovarian ci...

Descripción completa

Detalles Bibliográficos
Autores principales: Neyshaburinezhad, Navid, Hashemi, Maryam, Ramezani, Mohammad, Arabzadeh, Sepideh, Behravan, Javad, Kalalinia, Fatemeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251388/
https://www.ncbi.nlm.nih.gov/pubmed/30483395
http://dx.doi.org/10.22038/IJBMS.2018.29474.7118
_version_ 1783373113963053056
author Neyshaburinezhad, Navid
Hashemi, Maryam
Ramezani, Mohammad
Arabzadeh, Sepideh
Behravan, Javad
Kalalinia, Fatemeh
author_facet Neyshaburinezhad, Navid
Hashemi, Maryam
Ramezani, Mohammad
Arabzadeh, Sepideh
Behravan, Javad
Kalalinia, Fatemeh
author_sort Neyshaburinezhad, Navid
collection PubMed
description OBJECTIVE(S): Crocetin, one of the main substances of saffron extract, has anti-cancer effects. Drug resistance proteins (e.g. MRP1 and MRP2) are important reasons for the failure of cancer therapy. We intended to investigate the efficacy of crocetin on MRP(1) and MRP(2) activity in human ovarian cisplatin-resistant carcinoma cell line (A2780-RCIS). MATERIALS AND METHODS: The cytotoxic effect of crocetin was evaluated by the MTT assay. The efficacy of crocetin on MRP(1) and MRP(2) expression at mRNA level was studied by real-time RT-PCR. The effect of crocetin on the activity of MRP transporters was determined by drug efflux assay. RESULTS: Crocetin decreased cell proliferation in the A2780 (IC(50): 183±7 µM) and A2780-RCIS (IC(50): 316±9 µM). Crocetin decreased the expression level of MRP1 (22±2 %) and MRP2 (48±8 %) in A2780-RCIS and inhibited MRP pumps function directly in A2780 (44±1 %) and A2780-RCIS (88±10 %) and indirectly in A2780 (32±2 %) and A2780-RCIS (48±15 %) respectively. CONCLUSION: Our findings showed that crocetin could quench drug resistance through modulation of MRP transporters in the drug resistant human ovarian cancer cells.
format Online
Article
Text
id pubmed-6251388
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Mashhad University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-62513882018-11-27 The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype Neyshaburinezhad, Navid Hashemi, Maryam Ramezani, Mohammad Arabzadeh, Sepideh Behravan, Javad Kalalinia, Fatemeh Iran J Basic Med Sci Short Communication OBJECTIVE(S): Crocetin, one of the main substances of saffron extract, has anti-cancer effects. Drug resistance proteins (e.g. MRP1 and MRP2) are important reasons for the failure of cancer therapy. We intended to investigate the efficacy of crocetin on MRP(1) and MRP(2) activity in human ovarian cisplatin-resistant carcinoma cell line (A2780-RCIS). MATERIALS AND METHODS: The cytotoxic effect of crocetin was evaluated by the MTT assay. The efficacy of crocetin on MRP(1) and MRP(2) expression at mRNA level was studied by real-time RT-PCR. The effect of crocetin on the activity of MRP transporters was determined by drug efflux assay. RESULTS: Crocetin decreased cell proliferation in the A2780 (IC(50): 183±7 µM) and A2780-RCIS (IC(50): 316±9 µM). Crocetin decreased the expression level of MRP1 (22±2 %) and MRP2 (48±8 %) in A2780-RCIS and inhibited MRP pumps function directly in A2780 (44±1 %) and A2780-RCIS (88±10 %) and indirectly in A2780 (32±2 %) and A2780-RCIS (48±15 %) respectively. CONCLUSION: Our findings showed that crocetin could quench drug resistance through modulation of MRP transporters in the drug resistant human ovarian cancer cells. Mashhad University of Medical Sciences 2018-11 /pmc/articles/PMC6251388/ /pubmed/30483395 http://dx.doi.org/10.22038/IJBMS.2018.29474.7118 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Neyshaburinezhad, Navid
Hashemi, Maryam
Ramezani, Mohammad
Arabzadeh, Sepideh
Behravan, Javad
Kalalinia, Fatemeh
The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype
title The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype
title_full The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype
title_fullStr The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype
title_full_unstemmed The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype
title_short The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype
title_sort effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251388/
https://www.ncbi.nlm.nih.gov/pubmed/30483395
http://dx.doi.org/10.22038/IJBMS.2018.29474.7118
work_keys_str_mv AT neyshaburinezhadnavid theeffectsofcrocetinextractedfromsaffroninchemotherapyagainsttheincidenceofmultipledrugresistancephenotype
AT hashemimaryam theeffectsofcrocetinextractedfromsaffroninchemotherapyagainsttheincidenceofmultipledrugresistancephenotype
AT ramezanimohammad theeffectsofcrocetinextractedfromsaffroninchemotherapyagainsttheincidenceofmultipledrugresistancephenotype
AT arabzadehsepideh theeffectsofcrocetinextractedfromsaffroninchemotherapyagainsttheincidenceofmultipledrugresistancephenotype
AT behravanjavad theeffectsofcrocetinextractedfromsaffroninchemotherapyagainsttheincidenceofmultipledrugresistancephenotype
AT kalaliniafatemeh theeffectsofcrocetinextractedfromsaffroninchemotherapyagainsttheincidenceofmultipledrugresistancephenotype
AT neyshaburinezhadnavid effectsofcrocetinextractedfromsaffroninchemotherapyagainsttheincidenceofmultipledrugresistancephenotype
AT hashemimaryam effectsofcrocetinextractedfromsaffroninchemotherapyagainsttheincidenceofmultipledrugresistancephenotype
AT ramezanimohammad effectsofcrocetinextractedfromsaffroninchemotherapyagainsttheincidenceofmultipledrugresistancephenotype
AT arabzadehsepideh effectsofcrocetinextractedfromsaffroninchemotherapyagainsttheincidenceofmultipledrugresistancephenotype
AT behravanjavad effectsofcrocetinextractedfromsaffroninchemotherapyagainsttheincidenceofmultipledrugresistancephenotype
AT kalaliniafatemeh effectsofcrocetinextractedfromsaffroninchemotherapyagainsttheincidenceofmultipledrugresistancephenotype