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The presence of extracellular microRNAs in the media of cultured Drosophila cells
While regulatory RNA pathways, such as RNAi, have commonly been described at an intracellular level, studies investigating extracellular RNA species in insects are lacking. In the present study, we demonstrate the presence of extracellular microRNAs (miRNAs) in the cell-free conditioned media of two...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251921/ https://www.ncbi.nlm.nih.gov/pubmed/30470777 http://dx.doi.org/10.1038/s41598-018-35531-z |
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author | Van den Brande, Stijn Gijbels, Marijke Wynant, Niels Santos, Dulce Mingels, Lina Gansemans, Yannick Van Nieuwerburgh, Filip Vanden Broeck, Jozef |
author_facet | Van den Brande, Stijn Gijbels, Marijke Wynant, Niels Santos, Dulce Mingels, Lina Gansemans, Yannick Van Nieuwerburgh, Filip Vanden Broeck, Jozef |
author_sort | Van den Brande, Stijn |
collection | PubMed |
description | While regulatory RNA pathways, such as RNAi, have commonly been described at an intracellular level, studies investigating extracellular RNA species in insects are lacking. In the present study, we demonstrate the presence of extracellular microRNAs (miRNAs) in the cell-free conditioned media of two Drosophila cell lines. More specifically, by means of quantitative real-time PCR (qRT-PCR), we analysed the presence of twelve miRNAs in extracellular vesicles (EVs) and in extracellular Argonaute-1 containing immunoprecipitates, obtained from the cell-free conditioned media of S2 and Cl.8 cell cultures. Next-generation RNA-sequencing data confirmed our qRT-PCR results and provided evidence for selective miRNA secretion in EVs. To our knowledge, this is the first time that miRNAs have been identified in the extracellular medium of cultured cells derived from insects, the most speciose group of animals. |
format | Online Article Text |
id | pubmed-6251921 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62519212018-11-30 The presence of extracellular microRNAs in the media of cultured Drosophila cells Van den Brande, Stijn Gijbels, Marijke Wynant, Niels Santos, Dulce Mingels, Lina Gansemans, Yannick Van Nieuwerburgh, Filip Vanden Broeck, Jozef Sci Rep Article While regulatory RNA pathways, such as RNAi, have commonly been described at an intracellular level, studies investigating extracellular RNA species in insects are lacking. In the present study, we demonstrate the presence of extracellular microRNAs (miRNAs) in the cell-free conditioned media of two Drosophila cell lines. More specifically, by means of quantitative real-time PCR (qRT-PCR), we analysed the presence of twelve miRNAs in extracellular vesicles (EVs) and in extracellular Argonaute-1 containing immunoprecipitates, obtained from the cell-free conditioned media of S2 and Cl.8 cell cultures. Next-generation RNA-sequencing data confirmed our qRT-PCR results and provided evidence for selective miRNA secretion in EVs. To our knowledge, this is the first time that miRNAs have been identified in the extracellular medium of cultured cells derived from insects, the most speciose group of animals. Nature Publishing Group UK 2018-11-23 /pmc/articles/PMC6251921/ /pubmed/30470777 http://dx.doi.org/10.1038/s41598-018-35531-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Van den Brande, Stijn Gijbels, Marijke Wynant, Niels Santos, Dulce Mingels, Lina Gansemans, Yannick Van Nieuwerburgh, Filip Vanden Broeck, Jozef The presence of extracellular microRNAs in the media of cultured Drosophila cells |
title | The presence of extracellular microRNAs in the media of cultured Drosophila cells |
title_full | The presence of extracellular microRNAs in the media of cultured Drosophila cells |
title_fullStr | The presence of extracellular microRNAs in the media of cultured Drosophila cells |
title_full_unstemmed | The presence of extracellular microRNAs in the media of cultured Drosophila cells |
title_short | The presence of extracellular microRNAs in the media of cultured Drosophila cells |
title_sort | presence of extracellular micrornas in the media of cultured drosophila cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251921/ https://www.ncbi.nlm.nih.gov/pubmed/30470777 http://dx.doi.org/10.1038/s41598-018-35531-z |
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