Rapid and Simple Detection of Escherichia coli by Loop-Mediated Isothermal Amplification Assay in Urine Specimens

BACKGROUND: To improve urinary tract infection detection, we evaluated the specificity and sensitivity of Loop-mediated isothermal Amplification Method (LAMP) for detection of the Eschericia coli (E. coli) in urine samples, for the first time. METHODS: Primers were designed to target the malB gene o...

Descripción completa

Detalles Bibliográficos
Autores principales: Ramezani, Reihaneh, Kardoost Parizi, Zahra, Ghorbanmehr, Nassim, Mirshafiee, Hamideh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2018
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252025/
https://www.ncbi.nlm.nih.gov/pubmed/30555663
_version_ 1783373197906804736
author Ramezani, Reihaneh
Kardoost Parizi, Zahra
Ghorbanmehr, Nassim
Mirshafiee, Hamideh
author_facet Ramezani, Reihaneh
Kardoost Parizi, Zahra
Ghorbanmehr, Nassim
Mirshafiee, Hamideh
author_sort Ramezani, Reihaneh
collection PubMed
description BACKGROUND: To improve urinary tract infection detection, we evaluated the specificity and sensitivity of Loop-mediated isothermal Amplification Method (LAMP) for detection of the Eschericia coli (E. coli) in urine samples, for the first time. METHODS: Primers were designed to target the malB gene of Escherichia coli. LAMP assay was performed on urine specimens collected from patients with urinary tract infection symptoms. RESULTS: As expected, LAMP was more specific and sensitive than direct microscopic tests. LAMP assay showed the best detection limit of DNA copies with 1.02 copies. CONCLUSION: LAMP method offers several advantages in terms of sensitivity, rapidness and simplicity for detection of E. coli infection in urine samples. The LAMP method would be highly suitable for the early detection of the UTIs and also comfort quick diagnosis of UTI in clinical laboratories with limited equipment.
format Online
Article
Text
id pubmed-6252025
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Avicenna Research Institute
record_format MEDLINE/PubMed
spelling pubmed-62520252018-12-14 Rapid and Simple Detection of Escherichia coli by Loop-Mediated Isothermal Amplification Assay in Urine Specimens Ramezani, Reihaneh Kardoost Parizi, Zahra Ghorbanmehr, Nassim Mirshafiee, Hamideh Avicenna J Med Biotechnol Short Communication BACKGROUND: To improve urinary tract infection detection, we evaluated the specificity and sensitivity of Loop-mediated isothermal Amplification Method (LAMP) for detection of the Eschericia coli (E. coli) in urine samples, for the first time. METHODS: Primers were designed to target the malB gene of Escherichia coli. LAMP assay was performed on urine specimens collected from patients with urinary tract infection symptoms. RESULTS: As expected, LAMP was more specific and sensitive than direct microscopic tests. LAMP assay showed the best detection limit of DNA copies with 1.02 copies. CONCLUSION: LAMP method offers several advantages in terms of sensitivity, rapidness and simplicity for detection of E. coli infection in urine samples. The LAMP method would be highly suitable for the early detection of the UTIs and also comfort quick diagnosis of UTI in clinical laboratories with limited equipment. Avicenna Research Institute 2018 /pmc/articles/PMC6252025/ /pubmed/30555663 Text en Copyright© 2018 Avicenna Research Institute http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Ramezani, Reihaneh
Kardoost Parizi, Zahra
Ghorbanmehr, Nassim
Mirshafiee, Hamideh
Rapid and Simple Detection of Escherichia coli by Loop-Mediated Isothermal Amplification Assay in Urine Specimens
title Rapid and Simple Detection of Escherichia coli by Loop-Mediated Isothermal Amplification Assay in Urine Specimens
title_full Rapid and Simple Detection of Escherichia coli by Loop-Mediated Isothermal Amplification Assay in Urine Specimens
title_fullStr Rapid and Simple Detection of Escherichia coli by Loop-Mediated Isothermal Amplification Assay in Urine Specimens
title_full_unstemmed Rapid and Simple Detection of Escherichia coli by Loop-Mediated Isothermal Amplification Assay in Urine Specimens
title_short Rapid and Simple Detection of Escherichia coli by Loop-Mediated Isothermal Amplification Assay in Urine Specimens
title_sort rapid and simple detection of escherichia coli by loop-mediated isothermal amplification assay in urine specimens
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252025/
https://www.ncbi.nlm.nih.gov/pubmed/30555663
work_keys_str_mv AT ramezanireihaneh rapidandsimpledetectionofescherichiacolibyloopmediatedisothermalamplificationassayinurinespecimens
AT kardoostparizizahra rapidandsimpledetectionofescherichiacolibyloopmediatedisothermalamplificationassayinurinespecimens
AT ghorbanmehrnassim rapidandsimpledetectionofescherichiacolibyloopmediatedisothermalamplificationassayinurinespecimens
AT mirshafieehamideh rapidandsimpledetectionofescherichiacolibyloopmediatedisothermalamplificationassayinurinespecimens

Ejemplares similares