Kinetics of Human Brown Adipose Tissue Activation and Deactivation

Brown adipose tissue (BAT) has been identified as a potential target in the treatment and prevention of obesity and metabolic disease. The precise kinetics of BAT activation and the duration of stimulus required to recruit metabolically active BAT, and its subsequent deactivation, are not well-understood. In this clinical trial, 19 healthy adults (BMI: 23.7±0.7 kg/m(2), Age: 31.2±2.8 y, 12 female) underwent three different cooling procedures to stimulate BAT glucose uptake, and active BAT volume was determined using (18)F-Fluorodeoxyglucose (FDG) PET/CT imaging. We found that 20 minutes of pre-injection cooling produces activation similar to the standard 60 minutes (39.9 mL vs. 44.2 mL, p= 0.52), indicating that BAT activity approaches its peak function soon after the initiation of cooling. Furthermore, upon removal of cold exposure, active BAT volume declines (13.6 mL vs. 44.2 mL, p=0.002), but the deactivation process persists even hours following cessation of cooling. Thus, the kinetics of human BAT thermogenesis are characterized by a rapid increase soon after cold stimulation but a more gradual decline after rewarming. These characteristics reinforce the feasibility of developing mild, short-duration cold exposure to activate BAT and treat obesity and metabolic disease.