Cargando…

Proteomic Analysis of Plasmodesmata From Populus Cell Suspension Cultures in Relation With Callose Biosynthesis

Plasmodesmata are channels that link adjacent cells in plant tissues through which molecular exchanges take place. They are involved in multiple processes vital to plant cells, such as responses to hormonal signaling or environmental challenges including osmotic stress, wounding and pathogen attack....

Descripción completa

Detalles Bibliográficos
Autores principales: Leijon, Felicia, Melzer, Michael, Zhou, Qi, Srivastava, Vaibhav, Bulone, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252348/
https://www.ncbi.nlm.nih.gov/pubmed/30510561
http://dx.doi.org/10.3389/fpls.2018.01681
_version_ 1783373242517422080
author Leijon, Felicia
Melzer, Michael
Zhou, Qi
Srivastava, Vaibhav
Bulone, Vincent
author_facet Leijon, Felicia
Melzer, Michael
Zhou, Qi
Srivastava, Vaibhav
Bulone, Vincent
author_sort Leijon, Felicia
collection PubMed
description Plasmodesmata are channels that link adjacent cells in plant tissues through which molecular exchanges take place. They are involved in multiple processes vital to plant cells, such as responses to hormonal signaling or environmental challenges including osmotic stress, wounding and pathogen attack. Despite the importance of plasmodesmata, their proteome is not well-defined. Here, we have isolated fractions enriched in plasmodesmata from cell suspension cultures of Populus trichocarpa and identified 201 proteins that are enriched in these fractions, thereby providing further insight on the multiple functions of plasmodesmata. Proteomics analysis revealed an enrichment of proteins specifically involved in responses to stress, transport, metabolism and signal transduction. Consistent with the role of callose deposition and turnover in the closure and aperture of the plasmodesmata and our proteomic analysis, we demonstrate the enrichment of callose synthase activity in the plasmodesmata represented by several gene products. A new form of calcium-independent callose synthase activity was detected, in addition to the typical calcium-dependent enzyme activity, suggesting a role of calcium in the regulation of plasmodesmata through two forms of callose synthase activities. Our report provides the first proteomic investigation of the plasmodesmata from a tree species and the direct biochemical evidence for the occurrence of several forms of active callose synthases in these structures. Data are available via ProteomeXchange with identifier PXD010692.
format Online
Article
Text
id pubmed-6252348
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-62523482018-12-03 Proteomic Analysis of Plasmodesmata From Populus Cell Suspension Cultures in Relation With Callose Biosynthesis Leijon, Felicia Melzer, Michael Zhou, Qi Srivastava, Vaibhav Bulone, Vincent Front Plant Sci Plant Science Plasmodesmata are channels that link adjacent cells in plant tissues through which molecular exchanges take place. They are involved in multiple processes vital to plant cells, such as responses to hormonal signaling or environmental challenges including osmotic stress, wounding and pathogen attack. Despite the importance of plasmodesmata, their proteome is not well-defined. Here, we have isolated fractions enriched in plasmodesmata from cell suspension cultures of Populus trichocarpa and identified 201 proteins that are enriched in these fractions, thereby providing further insight on the multiple functions of plasmodesmata. Proteomics analysis revealed an enrichment of proteins specifically involved in responses to stress, transport, metabolism and signal transduction. Consistent with the role of callose deposition and turnover in the closure and aperture of the plasmodesmata and our proteomic analysis, we demonstrate the enrichment of callose synthase activity in the plasmodesmata represented by several gene products. A new form of calcium-independent callose synthase activity was detected, in addition to the typical calcium-dependent enzyme activity, suggesting a role of calcium in the regulation of plasmodesmata through two forms of callose synthase activities. Our report provides the first proteomic investigation of the plasmodesmata from a tree species and the direct biochemical evidence for the occurrence of several forms of active callose synthases in these structures. Data are available via ProteomeXchange with identifier PXD010692. Frontiers Media S.A. 2018-11-19 /pmc/articles/PMC6252348/ /pubmed/30510561 http://dx.doi.org/10.3389/fpls.2018.01681 Text en Copyright © 2018 Leijon, Melzer, Zhou, Srivastava and Bulone. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Leijon, Felicia
Melzer, Michael
Zhou, Qi
Srivastava, Vaibhav
Bulone, Vincent
Proteomic Analysis of Plasmodesmata From Populus Cell Suspension Cultures in Relation With Callose Biosynthesis
title Proteomic Analysis of Plasmodesmata From Populus Cell Suspension Cultures in Relation With Callose Biosynthesis
title_full Proteomic Analysis of Plasmodesmata From Populus Cell Suspension Cultures in Relation With Callose Biosynthesis
title_fullStr Proteomic Analysis of Plasmodesmata From Populus Cell Suspension Cultures in Relation With Callose Biosynthesis
title_full_unstemmed Proteomic Analysis of Plasmodesmata From Populus Cell Suspension Cultures in Relation With Callose Biosynthesis
title_short Proteomic Analysis of Plasmodesmata From Populus Cell Suspension Cultures in Relation With Callose Biosynthesis
title_sort proteomic analysis of plasmodesmata from populus cell suspension cultures in relation with callose biosynthesis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252348/
https://www.ncbi.nlm.nih.gov/pubmed/30510561
http://dx.doi.org/10.3389/fpls.2018.01681
work_keys_str_mv AT leijonfelicia proteomicanalysisofplasmodesmatafrompopuluscellsuspensionculturesinrelationwithcallosebiosynthesis
AT melzermichael proteomicanalysisofplasmodesmatafrompopuluscellsuspensionculturesinrelationwithcallosebiosynthesis
AT zhouqi proteomicanalysisofplasmodesmatafrompopuluscellsuspensionculturesinrelationwithcallosebiosynthesis
AT srivastavavaibhav proteomicanalysisofplasmodesmatafrompopuluscellsuspensionculturesinrelationwithcallosebiosynthesis
AT bulonevincent proteomicanalysisofplasmodesmatafrompopuluscellsuspensionculturesinrelationwithcallosebiosynthesis