miR-205-5p Mediated Downregulation of PTEN Contributes to Cisplatin Resistance in C13K Human Ovarian Cancer Cells

Cisplatin resistance is a major cause of treatment failure in advanced ovarian cancer. The limited evidence shows the paradoxical regulation of miR-205 on chemotherapy resistance in cancer. Herein, we found that miR-205-5p was enormously increased in cisplatin-resistant C13K ovarian cancer cells com...

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Autores principales: Shi, Xiaoyan, Xiao, Lan, Mao, Xiaolu, He, Jinrong, Ding, Yu, Huang, Jin, Peng, Caixia, Xu, Zihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6253938/
https://www.ncbi.nlm.nih.gov/pubmed/30510566
http://dx.doi.org/10.3389/fgene.2018.00555
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author Shi, Xiaoyan
Xiao, Lan
Mao, Xiaolu
He, Jinrong
Ding, Yu
Huang, Jin
Peng, Caixia
Xu, Zihui
author_facet Shi, Xiaoyan
Xiao, Lan
Mao, Xiaolu
He, Jinrong
Ding, Yu
Huang, Jin
Peng, Caixia
Xu, Zihui
author_sort Shi, Xiaoyan
collection PubMed
description Cisplatin resistance is a major cause of treatment failure in advanced ovarian cancer. The limited evidence shows the paradoxical regulation of miR-205 on chemotherapy resistance in cancer. Herein, we found that miR-205-5p was enormously increased in cisplatin-resistant C13K ovarian cancer cells compared with its cisplatin-sensitive OV2008 parental cells using miRNA microarrays, which was further verified by quantitative PCR. Furthermore, we confirmed that inhibition of miR-205-5p upregulated PTEN and subsequently attenuated its downstream target p-AKT, which inversed C13K cells from cisplatin resistance to sensitivity. Our data suggest that miR-205-5p contributes to cisplatin resistance in C13K ovarian cancer cells may via targeting PTEN/AKT pathway.
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spelling pubmed-62539382018-12-03 miR-205-5p Mediated Downregulation of PTEN Contributes to Cisplatin Resistance in C13K Human Ovarian Cancer Cells Shi, Xiaoyan Xiao, Lan Mao, Xiaolu He, Jinrong Ding, Yu Huang, Jin Peng, Caixia Xu, Zihui Front Genet Genetics Cisplatin resistance is a major cause of treatment failure in advanced ovarian cancer. The limited evidence shows the paradoxical regulation of miR-205 on chemotherapy resistance in cancer. Herein, we found that miR-205-5p was enormously increased in cisplatin-resistant C13K ovarian cancer cells compared with its cisplatin-sensitive OV2008 parental cells using miRNA microarrays, which was further verified by quantitative PCR. Furthermore, we confirmed that inhibition of miR-205-5p upregulated PTEN and subsequently attenuated its downstream target p-AKT, which inversed C13K cells from cisplatin resistance to sensitivity. Our data suggest that miR-205-5p contributes to cisplatin resistance in C13K ovarian cancer cells may via targeting PTEN/AKT pathway. Frontiers Media S.A. 2018-11-19 /pmc/articles/PMC6253938/ /pubmed/30510566 http://dx.doi.org/10.3389/fgene.2018.00555 Text en Copyright © 2018 Shi, Xiao, Mao, He, Ding, Huang, Peng and Xu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Shi, Xiaoyan
Xiao, Lan
Mao, Xiaolu
He, Jinrong
Ding, Yu
Huang, Jin
Peng, Caixia
Xu, Zihui
miR-205-5p Mediated Downregulation of PTEN Contributes to Cisplatin Resistance in C13K Human Ovarian Cancer Cells
title miR-205-5p Mediated Downregulation of PTEN Contributes to Cisplatin Resistance in C13K Human Ovarian Cancer Cells
title_full miR-205-5p Mediated Downregulation of PTEN Contributes to Cisplatin Resistance in C13K Human Ovarian Cancer Cells
title_fullStr miR-205-5p Mediated Downregulation of PTEN Contributes to Cisplatin Resistance in C13K Human Ovarian Cancer Cells
title_full_unstemmed miR-205-5p Mediated Downregulation of PTEN Contributes to Cisplatin Resistance in C13K Human Ovarian Cancer Cells
title_short miR-205-5p Mediated Downregulation of PTEN Contributes to Cisplatin Resistance in C13K Human Ovarian Cancer Cells
title_sort mir-205-5p mediated downregulation of pten contributes to cisplatin resistance in c13k human ovarian cancer cells
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6253938/
https://www.ncbi.nlm.nih.gov/pubmed/30510566
http://dx.doi.org/10.3389/fgene.2018.00555
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