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Caring for cells in microsystems: principles and practices of cell-safe device design and operation

Microfluidic device designers and users continually question whether cells are ‘happy’ in a given microsystem or whether they are perturbed by micro-scale technologies. This issue is normally brought up by engineers building platforms, or by external reviewers (academic or commercial) comparing mult...

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Detalles Bibliográficos
Autores principales: Varma, Sarvesh, Voldman, Joel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254237/
https://www.ncbi.nlm.nih.gov/pubmed/30324208
http://dx.doi.org/10.1039/c8lc00746b
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author Varma, Sarvesh
Voldman, Joel
author_facet Varma, Sarvesh
Voldman, Joel
author_sort Varma, Sarvesh
collection PubMed
description Microfluidic device designers and users continually question whether cells are ‘happy’ in a given microsystem or whether they are perturbed by micro-scale technologies. This issue is normally brought up by engineers building platforms, or by external reviewers (academic or commercial) comparing multiple technological approaches to a problem. Microsystems can apply combinations of biophysical and biochemical stimuli that, although essential to device operation, may damage cells in complex ways. However, assays to assess the impact of microsystems upon cells have been challenging to conduct and have led to subjective interpretation and evaluation of cell stressors, hampering development and adoption of microsystems. To this end, we introduce a framework that defines cell health, describes how device stimuli may stress cells, and contrasts approaches to measure cell stress. Importantly, we provide practical guidelines regarding device design and operation to minimize cell stress, and recommend a minimal set of quantitative assays that will enable standardization in the assessment of cell health in diverse devices. We anticipate that as microsystem designers, reviewers, and end-users enforce such guidelines, we as a community can create a set of essential principles that will further the adoption of such technologies in clinical, translational and commercial applications.
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spelling pubmed-62542372018-12-19 Caring for cells in microsystems: principles and practices of cell-safe device design and operation Varma, Sarvesh Voldman, Joel Lab Chip Chemistry Microfluidic device designers and users continually question whether cells are ‘happy’ in a given microsystem or whether they are perturbed by micro-scale technologies. This issue is normally brought up by engineers building platforms, or by external reviewers (academic or commercial) comparing multiple technological approaches to a problem. Microsystems can apply combinations of biophysical and biochemical stimuli that, although essential to device operation, may damage cells in complex ways. However, assays to assess the impact of microsystems upon cells have been challenging to conduct and have led to subjective interpretation and evaluation of cell stressors, hampering development and adoption of microsystems. To this end, we introduce a framework that defines cell health, describes how device stimuli may stress cells, and contrasts approaches to measure cell stress. Importantly, we provide practical guidelines regarding device design and operation to minimize cell stress, and recommend a minimal set of quantitative assays that will enable standardization in the assessment of cell health in diverse devices. We anticipate that as microsystem designers, reviewers, and end-users enforce such guidelines, we as a community can create a set of essential principles that will further the adoption of such technologies in clinical, translational and commercial applications. Royal Society of Chemistry 2018-11-21 2018-10-16 /pmc/articles/PMC6254237/ /pubmed/30324208 http://dx.doi.org/10.1039/c8lc00746b Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by-nc/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0)
spellingShingle Chemistry
Varma, Sarvesh
Voldman, Joel
Caring for cells in microsystems: principles and practices of cell-safe device design and operation
title Caring for cells in microsystems: principles and practices of cell-safe device design and operation
title_full Caring for cells in microsystems: principles and practices of cell-safe device design and operation
title_fullStr Caring for cells in microsystems: principles and practices of cell-safe device design and operation
title_full_unstemmed Caring for cells in microsystems: principles and practices of cell-safe device design and operation
title_short Caring for cells in microsystems: principles and practices of cell-safe device design and operation
title_sort caring for cells in microsystems: principles and practices of cell-safe device design and operation
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6254237/
https://www.ncbi.nlm.nih.gov/pubmed/30324208
http://dx.doi.org/10.1039/c8lc00746b
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